EMAGE mouse gene expression spatial database

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EMAGE:32128

Agtpbp1 ( MGI:2159437)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007611_01	euxassay_007611_02	euxassay_007611_03	euxassay_007611_04	euxassay_007611_05

euxassay_007611_06	euxassay_007611_07	euxassay_007611_08	euxassay_007611_09	euxassay_007611_10

euxassay_007611_11	euxassay_007611_12	euxassay_007611_13	euxassay_007611_14	euxassay_007611_15

euxassay_007611_16	euxassay_007611_17	euxassay_007611_18	euxassay_007611_19	euxassay_007611_20

euxassay_007611_21	euxassay_007611_22	euxassay_007611_23	euxassay_007611_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 15 16 17

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 15 16

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21 22

ventral grey horn

weak

regional

weak expression: see section 09 10 11 12 13 14 15

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17 18

pons mantle layer

moderate

regional

moderate expression: see section 07 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35894
Entity Detected:	Agtpbp1, ( MGI:2159437)
Sequence:	sense strand is shown >T35894 ACGTGTTGTGTGAAACCTTGTCTGGAAACATCTGTCCTTTGGTGACCATAACAGCAATGCCAGAGTCCAA TTACTATGAACATATCTGTCAGTTCAGAACTCGCCCTTATATTTTCTTGTCTGCTCGGGTCCATCCTGGA GAAACCAATGCAAGCTGGGTAATGAAAGGAACACTGGAGTACCTCATGAGCAATAGCCCGACTGCCCAGA GCCTACGGGAGTCTTACATTTTTAAAATTGTCCCCATGCTAAATCCAGATGGTGTCATCAATGGAAATCA CCGCTGCTCCTTAAGTGGAGAGGGTTTGAACAGACAGTGGCAAAGTCCAAACCCAGAGTTACACCCCACG ATTTATCATGCCAAGGGGCTGCTGCAGTACCTGGCCGCGGTGAAGCGCCTACCTCTGGTTTATTGTGATT ACCATGGCCATTCTCGGAAAAAGAATGTATTCATGTACGGCTGCAGCATCAAAGAGACGGTGTGGCACAC CCATGACAACTCGGCTTCCTGTGATATTGTGGAAGGCATGGGATACAGGACTTTGCCTAAGATACTGAGC CACATTGCTCCG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91191. Forward Primer - name:091191_F_cDNA_Agtpbp1, sequence:ACGTGTTGTGTGAAACCTTGTC; Reverse Primer - name:091191_N_SP6_cDNA_Agtpbp1, sequence:CGGAGCAATGTGGCTCAGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007611 same experiment
	EMAGE:30855 same embryo
	EMAGE:30811 same embryo
	EMAGE:30807 same embryo
	EMAGE:30731 same embryo
	EMAGE:30730 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source