EMAGE mouse gene expression spatial database

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EMAGE:32131

Fbxo2 ( MGI:2446216)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004104_01	euxassay_004104_02	euxassay_004104_03	euxassay_004104_04	euxassay_004104_05

euxassay_004104_06	euxassay_004104_07	euxassay_004104_08	euxassay_004104_09	euxassay_004104_10

euxassay_004104_11	euxassay_004104_12	euxassay_004104_13	euxassay_004104_14	euxassay_004104_15

euxassay_004104_16	euxassay_004104_17	euxassay_004104_18	euxassay_004104_19	euxassay_004104_20

euxassay_004104_21	euxassay_004104_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5592
Entity Detected:	Fbxo2, ( MGI:2446216)
Sequence:	sense strand is shown >T5592 CTCTCTTGACAGCACCGCAGCGATGGATGGAGATGGTGATCCAGAGAGTGTGAGCCACCCCGAAGAAGCG AGCCCAGAGGAGCAGCCAGAGGAGGCGGGCGCCGAGGCGAGTGCGGAGGAGGAGCAGCTCCGGGAGGCGG AGGAGGAGGAAGAGGCGGAGGCCGTGGAGTACCTGGCCGAGCTGCCGGAGCCACTGCTGCTGCGCGTGCT GGCCGAGCTGCCAGCTACGGAGCTGGTGCAGGCCTGCCGCCTGGTGTGCCTGCGCTGGAAGGAGCTGGTG GACGGCGCCCCACTGTGGCTGCTCAAGTGCCAGCAGGAGGGGCTGGTGCCCGAGGGCAGCGCTGATGAGG AGCGGGACCACTGGCAACAGTTCTACTTTCTGAGCAAGAGGAGGCGCAACCTGCTGCGCAACCCTTGTGG GGAAGAGGACTTGGAGGGCTGGAGCGACGTGGAGCACGGTGGGGACGGCTGGAGGGTGGAGGAACTGCCC GGAGACAATGGGGTGGAATTTACCCAAGATGACAGCGTTAAGAAATACTTCGCCTCCTCCTTCGAGTGGT GTCGCAAAGCGCAGGTCATTGATCTGCAGGCAGAGGGCTACTGGGAGGAGCTACTGGACACCACCCAGCC CGCCATCGTGGTGAAGGACTGGTACTCGGGTCGCACTGATGCGGGCAGCCTGTATGAGCTCACTGTGAGG CTGCTGTCGGAGAACGAAGATGTGCTGGCGGAGTTCGCTACAGGACAGGTGGCTGTGCCAGAGGACGGCA GTTGGATGGAGATCTCCCACACCTTCATCGACTACGGGCCAGGTGTCCGCTTTGTCCGCTTCGAGCACGG AGGGCAGGACTCGGTCTACTGGAAGGGCTGGTTCGGGGCCCGGGTGACCAACAGTAGCGTGTGGGTGGAA CCCTGAACGACCTCCCCTACCCCCACCATCCGCCCTCTAAGGCAGAATTCTGGGGTAGAAAGGCCTTAAT TTAGTTGGTAGCATCCTCACCTGCCTCGCCCACGCCTTGCACCTGCCTCTCTCCCGCCCCTTCCTTCAGT CTGGTCCCGAGGAAAGAAGGACATGTTGCTTTACTGTAGGATGGCTTTCATTTGCATCTCCACGGGCAGT CGCTTCTAGAATGTACGATCCGAGGGGGTATGGGGCTGCTGCTGGATCCCGCGCTCCTAGCATTGTGCCT GCCTCAAAGTGGGCACTCAATAAATATTTGTCCAAAGAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6487759 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6487759 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004104 same experiment
	EMAGE:30285 same embryo
	EMAGE:29949 same embryo
	EMAGE:31272 same embryo
	EMAGE:29998 same embryo
	EMAGE:30169 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source