EMAGE mouse gene expression spatial database

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EMAGE:32189

AA408296 ( MGI:2138080)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003243_01	euxassay_003243_02	euxassay_003243_03	euxassay_003243_04	euxassay_003243_05

euxassay_003243_06	euxassay_003243_07	euxassay_003243_08	euxassay_003243_09	euxassay_003243_10

euxassay_003243_11	euxassay_003243_12	euxassay_003243_13	euxassay_003243_14	euxassay_003243_15

euxassay_003243_16	euxassay_003243_17	euxassay_003243_18	euxassay_003243_19	euxassay_003243_20

euxassay_003243_21	euxassay_003243_22	euxassay_003243_23	euxassay_003243_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw molar

moderate

regional

moderate expression: see section 08 19

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20 weak expression: see section 16

upper jaw molar

moderate

regional

moderate expression: see section 08 19 20

vibrissa

moderate

regional

moderate expression: see section 07 08 22

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4845
Entity Detected:	AA408296, ( MGI:2138080)
Sequence:	sense strand is shown >T4845 CCACGCGTCCGCCCACGCGTCCGGGGAACCTTGTGTTTTGTGTTTTGTTTGTTTGTTTGTGGCGAGCTTG GACGTTTCCGCTATGGGCAAACGCCGGAACCGGGGCCGCAGCCAGATGCTCAGCACCATGACCAAGAAGC AGAAGAAGCACCTCCGCGACTTCGGCGAGGAGCACCCCTTCTACGACAGGGTTTCCAAGAAGGAAGCCAA GCCGCAGATTTGTCAGCTGCCAGAGAGTTCAGATTCTTCACATTCTGAAAGTGAATCAGAGAGTGAACAA GAGCATGTTTCAGGGTACCACAGACTGCTTGCTACATTAAAGAATGTTTCTGAGGAAGAGGAAGAGGAAG AGGAGGAGGAGGAAGAAGAGGAAGATGACAGTGCTGTAGGTGATGCAGAAATGAATGAGGAAGCTGGCAG TGAGGATGGCAGTGTGGGAGAAGCTGCTGTCTCGGAGGCTGCTGAGGAGGCTGCTGAGACACAGGAACAT ATGTCTCTAGCTGACAACTCAAAAGAAAAAGATGGTGAAGAGCCACCTGGTGTATCACAGAAATCCTCAG AGGAGTTCACAGATGTGAAACACGAGTCACTGTTCAGCCTGGAAACTAATTTTCTAGAAGAGGATAGCGG GGGCAGTTGTTCCCAGAGGCCATCCCAAGATCCGTTTCAACAACATGTCAACAAAGAACTGAAAGAGAAG GAAATTCAAGCTGCTGCTTCCAGCCCTCCAGCTACCCAGCAACTGAAATGGCCAGTCCTGGGACATCTTG TCTTTTCATCAAAGTTCCAGAAGATGGAAACATTTAAACCTCCAAAGGATATTGACCTAAAGTCACTTCA TCTTCAGAAGCCTCTAGAATCCACCTGGGCAAAAACCAATAGCCAATTTCTATCTGGACCTCAAAAATCA AATAGCTCCTTTACCCCGCTCCAGAAAGAGCTGTTCTTAATTATGAATTCTTACCGGGACCTGTTCTACC CTGAGAGGACTGCCCTGAAGAATGGAGAAGAAGTCCGCCATGTATACTGCCTGCATGCCATAAACCATGT CCTCAAACCCAATGCTCAGGTGCTGGCTAACAACAGTAGGCGCCGGAGCCAGAAACTTGGGGTGGGTGAA GATGATGACTTCAGGGACCAAGGGCTCACCAGGCCTAAGGTACTTATAGTGGTGCCCTTCCGGGAAGCTG CCCTTCGGGTGGTCCAGCTCTTCATCAGCCTCCTTGAAGGGGACAGCAAGAAGAAAATCATTGTAAGCAA TAAAAAGCGGTTTCAGGGGGAGTATGGATCTGATCCTGAGGAGAGACCTCCCAACCTGAAGAGGCCTGAG GATTATGAAGCTGTGTTCGTGGGCAATATTGATGACCACTTCAGGATTGGAGTGGCAATACTTCAGAGGA GCATCCGTCTCTACGCCCCGTTTTACTCCTCCGACATCCTCATTGCCTCCCCCCTGGGCTTGAGGACCAT CATTGGTGGTGAAGGAGAGAAGAAGAGAGATTTTGACTTCCTGTCTTCCGTTGAGCTGCTCATCATTGAC CAGGCTGACATTTACCTGATGCAGAACTGGGAACATGTCTTGCATTTGATGAACCACATGAACCTGCTGC CCTTGGACTCACACGGGGTGGACTTCTCGAGAGTCCGAATGTGGAGCCTCAATAACTGGTCCAAGTACTA CCGACAGACACTGCTCTTTGGTGCCCTGCAGGATGCCCAGATCAACTCTGTGTTCAACAAGCACTGCATC AATGCTCAAGGCCAGGTGGCTGTGAGGAATGTCCCCATGACGGGCTCCATCAGTCATGTGTTGGTGCAGC TCCCTCACGTCTTCCAGAGAATGGAAGCACAAGACCTATCTTCAGTGATCGATGCCAGGTTTCACTTTTT CATAAACAAGATTTTGCCTCAGTACCGGGATGCTGTCATGTCTCACACACTCATCTATGTCCCCTCCTAC TTTGACTTTGTGCGTCTCCGAAATTACTTCAAGAAAGAAGAGCTGAACTTCACACACATCTGCGAGTATA CGCAGAAGTCTGGGATCTCCAGGGCTCGACACTTCTTTCTGCAAGGAGAGAAGCAGTTTCTGCTCCTCAC AGAACGCTTCCATTTCTACAAAAGGTACACAATAAAAGGCATCAGGAACCTGATATTCTACGAACTGCCA ACGTACCCTCACTTCTACAGTGAGGTCTGTAACATGCTGAGAGCCACCAGCAGAGGAGAGGAGGCCACGT GGACGTGCACCGTGCTGTATTCCAAGTATGATGCCCAGAGGCTGGCGGCTGTGGTTGGTGTGGAGCGGGC GGCACAGATGCTGCAGTCTCCAAAGAATGTCCACCTCTTCGTCACCGGAGAAAAATGAGTGTGGAGGGGA ACGGACTACATTGTTGGACCTCATAGACCTGATCTGTTCACTGCCCCAGGAACGGGCTCCAGGCAGTCTG CAGTCCTGTGTCACTGGAAAGTGCAGTGTGCAGTGCAGAGTGACCAGTGTGGGTCACCACAGCTGTCAGG GCCAAGAGCAGTCAGAGGCCTGCTACTCTCAGAAACTGACGCTTGTGCTTGCCACACTTTGTAAGCTATG ACTTCTCCATTAAGATTTTACTTTTTTAATTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5065857 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5065857 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003243 same experiment
	EMAGE:32141 same embryo
	EMAGE:32180 same embryo
	EMAGE:30744 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source