EMAGE mouse gene expression spatial database

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EMAGE:32192

Aqp4 ( MGI:107387)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002150_01	euxassay_002150_02	euxassay_002150_03	euxassay_002150_04	euxassay_002150_05

euxassay_002150_06	euxassay_002150_07	euxassay_002150_08	euxassay_002150_09	euxassay_002150_10

euxassay_002150_11	euxassay_002150_12	euxassay_002150_13	euxassay_002150_14	euxassay_002150_15

euxassay_002150_16	euxassay_002150_17	euxassay_002150_18	euxassay_002150_19	euxassay_002150_20

euxassay_002150_21	euxassay_002150_22	euxassay_002150_23	euxassay_002150_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

weak

regional

weak expression: see section 07 08 09

midbrain ventricular layer

moderate

regional

moderate expression: see section 11 weak expression: see section 13

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 14 15 16 moderate expression: see section 08 09 17 18

ventral grey horn

weak

regional

weak expression: see section 08 09 10

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 11 weak expression: see section 13

stomach

strong

regional

strong expression: see section 05 06 07 08 moderate expression: see section 04

pons ventricular layer

strong

regional

strong expression: see section 11 12 moderate expression: see section 10 weak expression: see section 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4582
Entity Detected:	Aqp4, ( MGI:107387)
Sequence:	sense strand is shown >T4582 TGAGTGACAGAGCTGCGGCAAGGCGGTGGGGTAAGTGTGGACATTCCTGCAGTAGAGAGAGCATCATGGT GGCTTTCAAAGGAGTCTGGACTCAGGCTTTCTGGAAGGCAGTCTCAGCAGAATTTCTGGCCACGCTTATC TTTGTTTTGCTCGGTGTGGGATCCACCATAAACTGGGGTGGCTCAGAAAACCCCTTACCTGTGGACATGG TCCTCATCTCCCTTTGCTTTGGACTCAGCATTGCTACCATGGTGCAGTGCTTTGGCCACATCAGTGGTGG CCACATCAATCCCGCTGTGACTGTAGCCATGGTGTGCACACGAAAGATCAGCATCGCTAAGTCCGTCTTC TACATCATTGCACAGTGCCTGGGGGCCATCATTGGAGCCGGCATCCTCTACCTGGTCACACCTCCCAGTG TGGTTGGAGGATTGGGAGTCACCACGGTTCATGGAAACCTCACCGCTGGCCATGGGCTCCTGGTGGAGTT AATAATCACTTTCCAGTTGGTGTTCACTATTTTTGCCAGCTGTGATTCCAAACGAACTGATGTTACTGGT TCAATAGCTTTAGCAATTGGATTTTCCGTTGCAATTGGACATTTGTTTGCAATCAATTATACTGGAGCCA GCATGAATCCAGCTCGATCTTTTGGACCCGCAGTTATCATGGGAAACTGGGCAAACCACTGGATATATTG GGTTGGACCAATCATGGGCGCTGTGCTGGCAGGTGCCCTTTATGAGTATGTCTTCTGTCCTGATGTGGAG CTCAAACGTCGCCTTAAGGAAGCCTTCAGCAAAGCCGCGCAGCAGACAAAAGGGAGCTACATGGAGGTGG AGGACAACCGGAGCCAAGTGGAGACGGAAGACTTGATCCTGAAGCCCGGAGTGGTGCATGTGATTGACAT TGACCGTGGAGAAGAGAAGAAGGGGAAAGACTCTTCGGGAGAGGTATTGTCTTCCGTATGACTAGAGGAC AGCACTGAAGGCAGAAGAGACTCCCTAGACCTGGCCTCAGATTTCCTGCCACCCATTAAGGAAACAGATT TGTTATAAATTAGACACTTGCGGGTTTCTTGCTTCACACCTTGTTACACAGTTTAAATAACACATATTTT ACTATTATCAATGGGGGGGGTGAGAAAAAGCCTATAATGGATATAAAATTTTAAAACAGAAATCTTTTTG AATGTATCCCCAAAGCAACTATGCAACTAGTGTATTGGCTTCCCTTTCATTAATAACTGAAATTATGAAC CAAGATCTGGTCAAGTTTTGCCGTGCAGAGCATATGGACACCTCTGTGAGGAAGCTGGCATTGTCCATCG TCTTGACTATTGACTTCATTGGATTGATTTAAAAATGACAAAATGCAGTATGTCACAGAATCATGTGCAT TCAGGAGAAGACATGCAGTAACTTCTTCCACGAGCTATTCCTTATTTATAAACTACCTCGGAGGGGGAAA ACATTAGCAAGGGCCATTGCTAATACATCATTTGTATTTATATATCTGACTGTCAGAACCTAACTCTAAT TCACTATAATCCTCTCCCCCTCAGAATCCAAGAACCCATAGGGCTAAAAGTAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4985265 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4985265 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002150 same experiment
	EMAGE:31518 same embryo
	EMAGE:31460 same embryo
	EMAGE:31827 same embryo
	EMAGE:31721 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source