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EMAGE:3435

Nkx2-1 NK2 homeobox 1 ( MGI:108067)
TS18 (36-38 Somite no.)
immunohistochemistry

Data Images
EMAGE:3435 EMAGE:3435 EMAGE:3435 EMAGE:3435 EMAGE:3435
Figure 2A of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 2B of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 2C of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 2D of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 2E of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.
EMAGE:3435
Figure 2F of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
***Note: these sections are double stained for GSH2 (green) and NKX2-1(TITF1) (red). Regions of overlap in expression appear yellow. Only the data for NKX2-1(TITF1) has been annotated in this EMAGE entry.*** The sections are coronal telencephalic sections shown from anterior (A) to posterior (F). Image annotations: CGE, presumptive future caudal ganglionic eminence; Di, diencephalon; Sep, septum. Morphologically, embryo matches TS18 more closely than TS17 (35-39 somites).
Expression Pattern Description
Spatial Annotation:
EMAGE:3435EMAGE:3435EMAGE:3435EMAGE:3435EMAGE:3435Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mapping3D mapping3D mapping3D mapping
EMAGE:3435EMAGE:3435
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3435_voxel_notDetected_3D_1.wlz
3435_voxel_moderate_3D_1.wlz
3435_voxel_possible_3D_1.wlz
3435_voxel_notDetected_3D_2.wlz
3435_voxel_strong_3D_1.wlz
3435_voxel_moderate_3D_2.wlz
3435_voxel_notDetected_3D_3.wlz
3435_voxel_strong_3D_2.wlz
3435_voxel_notDetected_3D_4.wlz
3435_voxel_strong_3D_3.wlz
3435_voxel_possible_3D_2.wlz
3435_voxel_notDetected_3D_5.wlz
3435_voxel_strong_3D_4.wlz
3435_voxel_possible_3D_3.wlz
3435_voxel_notDetected_3D_6.wlz
3435_voxel_strong_3D_5.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3435_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
regionalA. Not expressed in anterior telencephalon. B. The most anterior expression is at the level of the septum. F. No expression at most posterior level of the telencephalon, expression elsewhere.
diencephalon
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:2676172
Entity Detected:Nkx2-1, NK2 homeobox 1 ( MGI:108067)
Notes:The anti-Titf1(Nkx2.1) antibody used in this study by Corbin et al., 2003 [PMID:12930780] was either a "mouse anti-Nkx2.1 (1:2000, DAKO, CA, USA)" or a "rabbit anti-Nkx2.1 (1:150, Biopat, Italy)". It is unspecified which of these were used in this actual experiment.
Antibody Type:monoclonal
Specimen
Organism:mouse
Age:36-38 Somite no.
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
General Information
Authors:Corbin JG; Rutlin M; Gaiano N; Fishell G, 2003 [PMID:12930780] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00717] [ PMID:12930780] Corbin JG, Rutlin M, Gaiano N, Fishell G 2003 Combinatorial function of the homeodomain proteins Nkx2.1 and Gsh2 in ventral telencephalic patterning. Development (130):4895-906
Links:MGI:2676233 same experiment
 EMAGE:3336 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI