EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:6090

F2rl2 coagulation factor II (thrombin) receptor-like 2 ( MGI:1298208)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008365. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008365_01.jpg	euxassay_008365_02.jpg	euxassay_008365_03.jpg	euxassay_008365_04.jpg

euxassay_008365_05.jpg	euxassay_008365_06.jpg	euxassay_008365_07.jpg	euxassay_008365_08.jpg	euxassay_008365_09.jpg

euxassay_008365_10.jpg	euxassay_008365_11.jpg	euxassay_008365_12.jpg	euxassay_008365_13.jpg	euxassay_008365_14.jpg

euxassay_008365_15.jpg	euxassay_008365_16.jpg	euxassay_008365_17.jpg	euxassay_008365_18.jpg	euxassay_008365_19.jpg

euxassay_008365_20.jpg	euxassay_008365_21.jpg	euxassay_008365_22.jpg	euxassay_008365_23.jpg

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6090_wholemount__moderate.wlz

6090_wholemount__notDetected.wlz

6090_wholemount__possible.wlz

6090_wholemount__strong.wlz

6090_wholemount__weak.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6090_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

strong

spotted

see section: 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

strong

spotted

see section: 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35158
Entity Detected:	F2rl2, coagulation factor II (thrombin) receptor-like 2 ( MGI:1298208)
Sequence:	sense strand is shown >T35158 CCCGAGGACAGTATTTCAACTCTCCACGTGAATAATGCTACCATAGGATACCTGAGAAGTTCCTTAAGTA CCCAAGTGATACCTGCCATCTACATCCTGCTGTTTGTGGTTGGTGTACCAGCCAACATCGTGACCCTGTG GAAACTCTCCTTAAGGACCAAATCCATCAGTCTGGTCATCTTTCACACCAACCTGGCCATCGCAGATCTC CTTTTCTGTGTCACACTGCCATTTAAGATCGCCTACCATCTCAATGGCAACAACTGGGTATTTGGCGAGG TCACGTGCCGGATCACCACGGTTGTTTTCTACGGCAACATGTACTGCGCTATCCTGATCCTCACCTGCAT GGGCATCAACCGCTACCTGGCCACGGCTCACCCTTTCACATACCAGAAGCTGCCCAAACGCAGCTTCTCC ATGCTCATGTGTGGCATGGTGTGGGTCATGGTTTTCTTATACATGCTGCCCTTTGTCATCCTGAAGCAGG AGTACCACCTCGTCCACTCCGAGATCACCACCTGCCACGATGTCGTTGACGCGTGCGAGTCCCCATCATC CTTCCGATTCTACTACTTCGTCTCCTTAGCATTCTTTGGGTTCCTCATCCCGTTTGTGATCATCATCTTC TGTTACACGACTCTCATCCACAAACTTAAATCAAAGGATCGGATATGGCTGGGCTACATCAAGGCCGTCC TCCTCATCCTTGTGATTTTCACAATTTGCTTTGCCCCCACCAACATCATACTCGTAATCCACCATGCCAA CTACTACTACCACAATACCGACAGCTTGTACTTTATGTATCTTATTGCTCTGTGCCTGGGGAGCCTGAAT AGCTGCCTAGATCCATTCCTTTACTTTGTCATGTCGAAAGTTGTAGATCAGCTTAATCCTTAGTCAGCAA TGGCAAGACCACCTTAGAGACCAAGGAGAGA
	nt 352 - nt 1292 of NM_010170.4
Notes:	The probe was transcribed from a PCR amplified fragment. The PCR template was a cDNA pool representing a variety of embryonic and postembryonic tissues, and the primers used were: 101359_F_cDNA_F2rl2 CCCGAGGACAGTATTTCAACTC (fwd) and 101359_N_SP6_cDNA_F2rl2 GCGATTTAGGTGACACTATAGTCTCTCCTTGGTCTCTAAGGTGG (rev).
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Acknowledgments:	Eurexpress Consortium
Links:	EurExpress:euxassay_008365 same experiment
	MGI:4824663 same experiment
	EMAGE:21043 same embryo
	EMAGE:21042 same embryo
	EMAGE:21045 same embryo
	EMAGE:21044 same embryo
	EMAGE:21047 same embryo
	EMAGE:21046 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source