EMAGE mouse gene expression spatial database

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EMAGE:6273

A930018M24Rik RIKEN cDNA A930018M24 gene ( MGI:2686053)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016065. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016065_01	euxassay_016065_02	euxassay_016065_03	euxassay_016065_04

euxassay_016065_05	euxassay_016065_06	euxassay_016065_07	euxassay_016065_08	euxassay_016065_09

euxassay_016065_10	euxassay_016065_11	euxassay_016065_12	euxassay_016065_13	euxassay_016065_14

euxassay_016065_15	euxassay_016065_16	euxassay_016065_17	euxassay_016065_18	euxassay_016065_19

euxassay_016065_20	euxassay_016065_21	euxassay_016065_22	euxassay_016065_23	euxassay_016065_24

euxassay_016065_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6273_wholemount_strong.wlz

6273_wholemount_moderate.wlz

6273_wholemount_weak.wlz

6273_wholemount_possible.wlz

6273_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6273_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 03 04 05 06 07 08 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 11 weak expression: see section 10 16

pons mantle layer

weak

regional

weak expression: see section 09 18

facial vii ganglion

moderate

regional

moderate expression: see section 06 weak expression: see section 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 17 18 19 weak expression: see section 20 21 22

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 17 18 19

mandible

weak

regional

weak expression: see section 06 07 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39861
Entity Detected:	A930018M24Rik, RIKEN cDNA A930018M24 gene ( MGI:2686053)
Sequence:	sense strand is shown >T39861 GTTTCAGGAAACCTTCCCTTTCTAGCTTCATCTCCGGAGAAAGGGAAGAAGCAGGATGACAGCGACGACT CAGCGGAGCCTGAGATGCTGTGCAGCGTGGAGCACCCCTGCCAGTTCTTCGCTGAAGCGCAGAGACTGCG GGAGCAGAAGCTGCTGCTGGATGAAGAGGTGTCTGTGGGGGGACAGGTATATGGGGTGCATCGGGTGATC TTGGCTGCAATTAGCAGCCTCTTCCGAGGCAGGCTGCTGAGCGGTGGGGGTCAGCAGCCCAGTTTCAGCC TTGATGTGACCCCGAGGGGCTGGGAGGCTGCGCTGACCTTTGCCTACGAGGGGGTGCTGGGCCCCGCCTC ACAGGACGAAGTGCTGGCTGCTGCGGAGGCGCTGGGAGCACCCCGGGTGAAGAAGGCGGCCCAGCTGAAG CCAGAAGGACTTGGTAAAGCCAGGGGAAATGAAAAGAAGCTCAGCCAGGCAGAGGAGCTGAGGGAGAATC TGCACAGCATTGAGCGTCTGTATCGAGAGGGCATCGGGTGCGACTTGGAGCTGGAGGCAGAGGGCTACCG CCTGCGGGGTGAGAGTCTGTGAGACAACTGCCAAAAATGCATGTTTTGCTTGTTTTGCGGTGGTAGGGAT GGAACCTAAGGCCTTAATTCCACTCTACCATGGATCCACACCCCCTAGCCTTGCGAAATAGTTTTGTATT ATTGCTTCAGCAAGAATGTCCTTTTTGGTGCTTCTCCAGAGCCACCAAGAAATGCACATGCGCAGTTGAA TTGTTTTCCTAGACACCCGGACAGATTTGAACTGATAGATAGTTCAGGCAGTCCCCCCCGCCTCCCTGGT AGCTAGAATTTCCAGCCAGATGGCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 72942. Forward Primer - name:072942_F_cDNA_A930018M24Rik, sequence:GTTTCAGGAAACCTTCCCTTTC; Reverse Primer - name:072942_N_SP6_cDNA_A930018M24Rik, sequence:AGCCATCTGGCTGGAAATTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6275 same embryo
	EMAGE:6276 same embryo
	EMAGE:6274 same embryo
	EurExpress:euxassay_016065 same experiment
	MGI:4822845 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source