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EMAGE:6285

9530020I12Rik RIKEN cDNA 9530020I12 gene ( MGI:1924692)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6285 EMAGE:6285 EMAGE:6285 EMAGE:6285 EMAGE:6285
"Pseudo-wholemount" of euxassay_016125. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016125_01 euxassay_016125_02 euxassay_016125_03 euxassay_016125_04
EMAGE:6285 EMAGE:6285 EMAGE:6285 EMAGE:6285 EMAGE:6285
euxassay_016125_05 euxassay_016125_06 euxassay_016125_07 euxassay_016125_08 euxassay_016125_09
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euxassay_016125_10 euxassay_016125_11 euxassay_016125_12 euxassay_016125_13 euxassay_016125_14
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euxassay_016125_15 euxassay_016125_16 euxassay_016125_17 euxassay_016125_18 euxassay_016125_19
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euxassay_016125_20 euxassay_016125_21 euxassay_016125_22 euxassay_016125_23 euxassay_016125_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6285Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6285_wholemount_strong.wlz
6285_wholemount_moderate.wlz
6285_wholemount_weak.wlz
6285_wholemount_possible.wlz
6285_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6285_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40355
Entity Detected:9530020I12Rik, RIKEN cDNA 9530020I12 gene ( MGI:1924692)
Sequence:sense strand is shown

>T40355
CCATGGCTAGAATTTCCTTCACTGGTGCTTAGAGTATTAATCAAAAAATGAAATCTTCACTTGTGTCCAG
TAAAGTGTTCCATCCTGAGATCTTCTGCTCTGTTTGGACTTCTGCCACTGGAGCTGAACAGCAGAGAAGC
CAGGCTGGGTCAAAGCTCCTGGAGGCAGAAGCCTTAACCCTACTTCTGGAAGCCTCTGTTCACTGCTTGG
CTCGCGCGGCTCGTGCGCCCCCTCCCGAGCAGCACCAGAACTGCAGAGCCCAGCCCCCGAGAAAGAGTGA
GCTGCCAGGGTGGTCTGAATGGGAGGCAAAGCTCAAGGAACCTTCTCAGGAGTTGATTCTCCAGGACTAT
GGAAAGCAAAGAGCAGATCGTGAAATGATGCGGAGGGCGCCGCCTGCATGATCCTCGAGTTCCTGACGTT
CCTGGGCTCAGTTGACATGTCGGTGGGAAGGAAGGGCGGGTTGCATCCTGCCTGTCAAAGGGATAACTTC
TTAGCTGCCCTGCTGCTGGACCCTGACCTCGATCTATGGGCTTCTTCATGACTACTTGGATGGATGCAGA
GGTCCACCTCTGGAGCTCCACCAACTTCATCTTAGAGATTGAGCCTTAGTGAAGAGACACTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 83636. Forward Primer - name:083636_F_cDNA_9530020I12Rik, sequence:CCATGGCTAGAATTTCCTTCAC; Reverse Primer - name:083636_N_SP6_cDNA_9530020I12Rik, sequence:CAGTGTCTCTTCACTAAGGCTCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6287 same embryo
 EMAGE:6288 same embryo
 EMAGE:6284 same embryo
 EMAGE:6283 same embryo
 EMAGE:6286 same embryo
 EurExpress:euxassay_016125 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS