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EMAGE:6413

Ube2v2 ubiquitin-conjugating enzyme E2 variant 2 ( MGI:1917870)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6413 EMAGE:6413 EMAGE:6413 EMAGE:6413 EMAGE:6413
"Pseudo-wholemount" of euxassay_007283. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007283_01 euxassay_007283_02 euxassay_007283_03 euxassay_007283_04
EMAGE:6413 EMAGE:6413 EMAGE:6413 EMAGE:6413 EMAGE:6413
euxassay_007283_05 euxassay_007283_06 euxassay_007283_07 euxassay_007283_08 euxassay_007283_09
EMAGE:6413 EMAGE:6413 EMAGE:6413 EMAGE:6413 EMAGE:6413
euxassay_007283_10 euxassay_007283_11 euxassay_007283_12 euxassay_007283_13 euxassay_007283_14
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euxassay_007283_15 euxassay_007283_16 euxassay_007283_17 euxassay_007283_18 euxassay_007283_19
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euxassay_007283_20 euxassay_007283_21 euxassay_007283_22 euxassay_007283_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6413Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6413_wholemount_strong.wlz
6413_wholemount_moderate.wlz
6413_wholemount_weak.wlz
6413_wholemount_possible.wlz
6413_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6413_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
ubiquitousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2966
Entity Detected:Ube2v2, ubiquitin-conjugating enzyme E2 variant 2 ( MGI:1917870)
Sequence:sense strand is shown

>T2966
GGCCTCGAGCCAGATTCGGCACGAGGCTTATGATGTCCAAAGAAAATATGAAGCTTCCACAGCCTCCAGA
AGGACAGACGTACAACAACTAACTTTAGTGGATCTCAAACTTGTCTTAAATCAGCAACCTTCTACTCATG
TTAATGTCTTGATTAAATATCACCATGCACAATACCCACACAATAAGTAAAAGAATTGCAGCTGGTAAAC
ATGACCTGGACAGTTGTAAGAATATATTTAATATATGTACACCTATTATGTTTTCAGGTAACAAGAGACG
TGCAGCACAATTTTTCTTTCTATTAAAGCACTGTCATTTAAACATGAACCTGAAGTACTCAAAATGGAGT
TCAGGCTTTCAAGACAAAAGCATGACAAAAGAGTGTCATGTGGCCATGGAAACATTTGTTTTTCTGGAAA
GTAAAGTCTCAAGAAGGAAGAACAGAAATGGCATGCTTTATCATATTTAGAGCTGCAGTTGGAATTGTTT
AAAGTAGCAGATACAATGACTAGTCAACAATTGTGTTAATTCTTGAAGCGGTGTGGGTGCTGACTACTAT
TAGTACAAACA
Notes:The probe template was PCR amplified from IMAGE:1532341 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1532341 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6414 same embryo
 EMAGE:6410 same embryo
 EMAGE:6409 same embryo
 EMAGE:6412 same embryo
 EMAGE:6411 same embryo
 EurExpress:euxassay_007283 same experiment
 MGI:4829058 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS