EMAGE mouse gene expression spatial database

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EMAGE:6435

Iqcj IQ motif containing J ( MGI:3644166)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016387. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016387_01	euxassay_016387_02	euxassay_016387_03	euxassay_016387_04

euxassay_016387_05	euxassay_016387_06	euxassay_016387_07	euxassay_016387_08	euxassay_016387_09

euxassay_016387_10	euxassay_016387_11	euxassay_016387_12	euxassay_016387_13	euxassay_016387_14

euxassay_016387_15	euxassay_016387_16	euxassay_016387_17	euxassay_016387_18	euxassay_016387_19

euxassay_016387_20	euxassay_016387_21	euxassay_016387_22	euxassay_016387_23	euxassay_016387_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6435_wholemount_strong.wlz

6435_wholemount_moderate.wlz

6435_wholemount_weak.wlz

6435_wholemount_possible.wlz

6435_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6435_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon floor plate

moderate

regional

moderate expression: see section 13

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 14 weak expression: see section 10 11 12 15 16

medulla oblongata floor plate

moderate

regional

moderate expression: see section 13

metencephalon floor plate

moderate

regional

moderate expression: see section 13

midbrain floor plate

moderate

regional

moderate expression: see section 13

midbrain mantle layer

moderate

regional

moderate expression: see section 14 weak expression: see section 11 12

glossopharyngeal ix ganglion

weak

single cell

weak expression: see section 18 19

trigeminal v ganglion

weak

single cell

weak expression: see section 05 06 07 08 17 18 19 20 21

ventral grey horn

weak

single cell

weak expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45193
Entity Detected:	Iqcj, IQ motif containing J ( MGI:3644166)
Sequence:	sense strand is shown >T45193 GTCTCTTCAGACAAGCTGAGCAGCTCTGTCAGCATGAACACCTTCTCCGACAGCAGCACACCCGTGAGTG TCTCCCGGCCGCTTGCTTGGACTGTCTTGCACTGAGTATTGTTAGCCCAGGCTCTGGTTCCCAGAAAAGA TCTTGAGATGGTTTGTGATGGGGTGTGTGTGTGTGTGGAGGGGTGCTTATTTGCTGCATGGGAGCCCACA GTGAGGCTCTTGGCGTGGTAGAACTAAGACAAGATTCATGATACAAAAATGAACTCAATGCGTCCACAGA GTGGGGATTCTGTGCGACGTTGCTTCTCTATCCCTGAGGTGGGTGCGCCCCACCCCCCATTTCTTCTGGT GTGTTTTCTCTGGCATGTGCTTATGTGATGCTTGTCTGGTTGGAAAGTCATCTTCGTTATTAGGATTAGA AAGAGAACTTTTGGCAGGAAAAATTGAAGTTGATCACAGACTGAAATTGCTGAAATGGATTGAGTGTGGC TAAATGGGTTGCACAGACCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 188679. Forward Primer - name:188679_F_exon_D230050A05, sequence:GTCTCTTCAGACAAGCTGAGCA; Reverse Primer - name:188679_N_SP6_exon_D230050A05, sequence:GGGTCTGTGCAACCCATTTAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6436 same embryo
	EMAGE:6438 same embryo
	EMAGE:6437 same embryo
	EMAGE:6434 same embryo
	EurExpress:euxassay_016387 same experiment
	MGI:4825612 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source