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EMAGE:6600

Sh3rf1 SH3 domain containing ring finger 1 ( MGI:1913066)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6600 EMAGE:6600 EMAGE:6600 EMAGE:6600 EMAGE:6600
"Pseudo-wholemount" of euxassay_016453. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016453_01 euxassay_016453_02 euxassay_016453_03 euxassay_016453_04
EMAGE:6600 EMAGE:6600 EMAGE:6600 EMAGE:6600 EMAGE:6600
euxassay_016453_05 euxassay_016453_06 euxassay_016453_07 euxassay_016453_08 euxassay_016453_09
EMAGE:6600 EMAGE:6600 EMAGE:6600 EMAGE:6600 EMAGE:6600
euxassay_016453_10 euxassay_016453_11 euxassay_016453_12 euxassay_016453_13 euxassay_016453_14
EMAGE:6600 EMAGE:6600 EMAGE:6600 EMAGE:6600 EMAGE:6600
euxassay_016453_15 euxassay_016453_16 euxassay_016453_17 euxassay_016453_18 euxassay_016453_19
EMAGE:6600 EMAGE:6600 EMAGE:6600 EMAGE:6600
euxassay_016453_20 euxassay_016453_21 euxassay_016453_22 euxassay_016453_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6600Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6600_wholemount_strong.wlz
6600_wholemount_moderate.wlz
6600_wholemount_weak.wlz
6600_wholemount_possible.wlz
6600_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6600_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 10 11 13 14 weak expression: see section 03 04 05 09 15 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63289
Entity Detected:Sh3rf1, SH3 domain containing ring finger 1 ( MGI:1913066)
Sequence:sense strand is shown

>T63289
AGGGGAATACCTCAGTTACCGTGTGCCAAAGCATTATATAACTACGAAGGAAAAGAGCCCGGAGACCTTA
AGTTCAGCAAAGGCGACACCATCATTCTGCGCCGACAGGTGGATGAGAATTGGTACCACGGGGAAGTCAG
CGGGGTCCACGGCTTTTTCCCCACTAACTTCGTGCAGATCATCAAACCTTTACCTCAGCCCCCGCCTCAG
TGCAAAGCACTTTACGACTTTGAAGTGAAAGACAAGGAAGCTGACAAAGATTGCCTTCCCTTCGCAAAGG
ACGACGTACTGACCGTGATCCGCAGAGTGGATGAAAACTGGGCTGAAGGAATGCTGGCAGATAAAATAGG
AATATTTCCAATTTCATACGTGGAGTTTAACTCAGCTGCCAAGCAGCTGATAGAGTGGGATAAGCCTCCC
GTGCCAGGAGTGGACACGGCAGAATGCCCCTCAGCGACGGCGCAGAGCACCTCTGCCTCAAAGCACCCCG
ACACCAAGAAGAACACCAGGAAGCGACACTCCTTCACCTCCCTCACCATGGCCAACAAGTCTTCCCAGGG
GTCCCAGAACCGCCACTCCATGGAGATCAGCCCTCCTGTGCTCATCAGTTCCAGCAACCCCACAGCCGCA
GCCCGCATCAGCGAACTGTCCGGGCTCTCCTGCAGCGCCCCGTCTCAGGTCCATATAAGCACCACTGGGT
TAATTGTGACCCCACCCCCTAGCAGCCCGGTGACAACTGGCCCTGCGTTCACGTTCCCTTCAGATGTCCC
CTACCAAGCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 104207. Forward Primer - name:104207_F_cDNA_Sh3md2, sequence:AGGGGAATACCTCAGTTACCGT; Reverse Primer - name:104207_N_SP6_cDNA_Sh3md2, sequence:CAGCTTGGTAGGGGACATCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6599 same embryo
 EMAGE:6603 same embryo
 EMAGE:6602 same embryo
 EMAGE:6601 same embryo
 EurExpress:euxassay_016453 same experiment
 MGI:4828044 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS