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EMAGE:6615

Csnka2ip casein kinase 2, alpha prime interacting protein ( MGI:2676295)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6615 EMAGE:6615 EMAGE:6615 EMAGE:6615 EMAGE:6615
"Pseudo-wholemount" of euxassay_016467. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016467_01 euxassay_016467_02 euxassay_016467_03 euxassay_016467_04
EMAGE:6615 EMAGE:6615 EMAGE:6615 EMAGE:6615 EMAGE:6615
euxassay_016467_05 euxassay_016467_06 euxassay_016467_07 euxassay_016467_08 euxassay_016467_09
EMAGE:6615 EMAGE:6615 EMAGE:6615 EMAGE:6615 EMAGE:6615
euxassay_016467_10 euxassay_016467_11 euxassay_016467_12 euxassay_016467_13 euxassay_016467_14
EMAGE:6615 EMAGE:6615 EMAGE:6615 EMAGE:6615 EMAGE:6615
euxassay_016467_15 euxassay_016467_16 euxassay_016467_17 euxassay_016467_18 euxassay_016467_19
EMAGE:6615 EMAGE:6615 EMAGE:6615 EMAGE:6615 EMAGE:6615
euxassay_016467_20 euxassay_016467_21 euxassay_016467_22 euxassay_016467_23 euxassay_016467_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6615Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6615_wholemount_strong.wlz
6615_wholemount_moderate.wlz
6615_wholemount_weak.wlz
6615_wholemount_possible.wlz
6615_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6615_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38194
Entity Detected:Csnka2ip, casein kinase 2, alpha prime interacting protein ( MGI:2676295)
Sequence:sense strand is shown

>T38194
CCTGCATTATTAAAGGCGGTAGTGTTCCTGTTGATGTTGTCAATAAAATTATCAATTCCATCTCCAAGTC
TACAATCCAGAAGGATCTCTCTAGGCAAATTCTCTTTCGAAGAATGAGAGGAAAGCCAAACCCTCGTCCT
GGTCCCCGCCTTTCATCAACATATTCTGTTTGTTTAGAGTGTGCTTCCGCCATAAAATCCCAGTGTAACC
ATCTTTCAGGAAAGAGAGATCCTCGATGCGCAACCCTCTTTGTCATACCAACACCCGAGAGCAGCACCGA
TGGGAAAGTAGATGTGAAAATAATCCTTATCCTTTCTCTACCAGAGAAGCCTTCCTCAACCTGTTTCCAA
CTTCCTATGAAAGACAGTAAATCTGAAAATAATTTAGAAGCCCTCGATGACAATCTGGACGTATTAGAGA
AGATTACACAGTTTTTCCCTGCATCCGAATCTGATTTTATCCAGGGGCTCAAGACAAAGCGAAAGTGTTT
GGCTGTATCCTCCGAGAGCAAAGATTTAAGCCAAGAGCCCCAGTCTATTGACTGGCTGCTGTATGTTAAA
AATAGTAATGGTATTCAGCTCGAAACCCAGGTCCAAGGCCCATCCTCCTCCTCTTCTTCCTCCTGCTCCT
CTGTTTCCTCCTCCTCCTCTGCTTCATCTATTGGCCGTCCCTCTCCACCCACTCCTTGGGTAGATCCCAC
TCCGGTTCCGGTTTCAGGATATGTTCTTGCAAAGGTGAGAAGTTATCACAGGTTACCTCCAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 105182. Forward Primer - name:105182_F_cDNA_Ckt2, sequence:CCTGCATTATTAAAGGCGGTAG; Reverse Primer - name:105182_N_SP6_cDNA_Ckt2, sequence:CCTGGAGGTAACCTGTGATAACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6617 same embryo
 EMAGE:6616 same embryo
 EMAGE:6618 same embryo
 EurExpress:euxassay_016467 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS