EMAGE mouse gene expression spatial database

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EMAGE:6631

Lrrcc1 leucine rich repeat and coiled-coil domain containing 1 ( MGI:1918960)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007263. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007263_01	euxassay_007263_02	euxassay_007263_03	euxassay_007263_04

euxassay_007263_05	euxassay_007263_06	euxassay_007263_07	euxassay_007263_08	euxassay_007263_09

euxassay_007263_10	euxassay_007263_11	euxassay_007263_12	euxassay_007263_13	euxassay_007263_14

euxassay_007263_15	euxassay_007263_16	euxassay_007263_17	euxassay_007263_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6631_wholemount_strong.wlz

6631_wholemount_moderate.wlz

6631_wholemount_weak.wlz

6631_wholemount_possible.wlz

6631_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6631_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pectoral girdle and thoracic body wall muscle

moderate

regional

moderate expression: see section 12 13 14

viscerocranium

moderate

regional

Expression in the turbinate bone.

aortic valve

moderate

regional

moderate expression: see section 12 13 14

mitral valve

moderate

regional

moderate expression: see section 12 13

tricuspid valve

moderate

regional

moderate expression: see section 14

meckel's cartilage

weak

regional

weak expression: see section 07 08 09 10 11 14 15 16 17 18

basisphenoid bone

weak

regional

weak expression: see section 03

exoccipital bone

weak

regional

weak expression: see section 03

temporal bone

weak

regional

weak expression: see section 03 04 05

vault of skull

weak

regional

weak expression: see section 02 03 04

orbito-sphenoid

weak

regional

weak expression: see section 02 03 06 07 08 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1916
Entity Detected:	Lrrcc1, leucine rich repeat and coiled-coil domain containing 1 ( MGI:1918960)
Sequence:	sense strand is shown >T1916 AAGTTTATGCAGAGAAAATGAATCTCTGAGAAAAAGCCATGAAAGTGACTGTGATGCATTGAGAATAAAG TGCAAGATCATTGAAGACCAAAATGAAACCATCCGGAAACTAAAAGACAGTTTACAAGAAAAAGATGGGC AAATCAAATTGCTACAAGAACAGATCGCTCTCATCGAAAAGTGTTCTCAAGAGCAACTTAATGAAAAGTC TTCACAACTAGATTCTATAGTTGAGAAACTAGAGAGACACAATGAGAGAAAGGAAAAATTAAAGCAACAG TTGAAAGCAAAGGAATTAGAACTTGAAGAAATCAGAAAAGCTTACAGCACACTAAATAAGAAATGGCATG ATAAAGGAGAACTACTCTCTCATCTTGAAATGCAAGTAAAAGAAGTAAAAGAAAAATTTGAAGACAAGGA AAGGAAACTGAAAGCAGAGAGAGACAAAAGTCTTGAGCTACAAAAGGATGCAATGGAAAAGCTTCAGAAC ATGGATGATGCCTTTAGAAGACAAGTGGATGAGA
Notes:	The probe template was PCR amplified from IMAGE:622823 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:622823 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6629 same embryo
	EMAGE:6628 same embryo
	EMAGE:6633 same embryo
	EMAGE:6632 same embryo
	EMAGE:6630 same embryo
	EurExpress:euxassay_007263 same experiment
	MGI:4826002 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source