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EMAGE:6639

Zfp281 zinc finger protein 281 ( MGI:3029290)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6639 EMAGE:6639 EMAGE:6639 EMAGE:6639 EMAGE:6639
"Pseudo-wholemount" of euxassay_007265. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007265_01 euxassay_007265_02 euxassay_007265_03 euxassay_007265_04
EMAGE:6639 EMAGE:6639 EMAGE:6639 EMAGE:6639 EMAGE:6639
euxassay_007265_05 euxassay_007265_06 euxassay_007265_07 euxassay_007265_08 euxassay_007265_09
EMAGE:6639 EMAGE:6639 EMAGE:6639 EMAGE:6639 EMAGE:6639
euxassay_007265_10 euxassay_007265_11 euxassay_007265_12 euxassay_007265_13 euxassay_007265_14
EMAGE:6639 EMAGE:6639 EMAGE:6639 EMAGE:6639 EMAGE:6639
euxassay_007265_15 euxassay_007265_16 euxassay_007265_17 euxassay_007265_18 euxassay_007265_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6639Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6639_wholemount_strong.wlz
6639_wholemount_moderate.wlz
6639_wholemount_weak.wlz
6639_wholemount_possible.wlz
6639_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6639_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vertebral axis musculature
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19
bladder
weak weak
regionalExpression in the fundus region.
urethra of male
moderate moderate
regionalmoderate expression: see section 11 12
lung
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1970
Entity Detected:Zfp281, zinc finger protein 281 ( MGI:3029290)
Sequence:sense strand is shown

>T1970
GGTTTTGGCCAGTCTGTTACCTCAGTGCTGCCATCTTCGTTGCCAAAGCCTCCTTTTGGGATGTTGTTTG
GATCTCAACCAGGTCTTTATTTATCTGCTTTGGATGCTACACATCAGCAGTTGACACCTTCCCAGGAGCT
GGATGATCTGATAGATTCTCAGAAGAACTTAGAGACATCATCAGCCTTCCAGTCCTCATCTCAGAAATTG
ACTAGCCAGAAGGAACAACAGAAAAATTTAGAGTCCTCAACGAGCTTTCAGATTCCATCTCAGGAGTTAG
CTAGCCAGATTGATCCTCAGAAAGACATAGAGCCTAGAACAACGTACCAGATTGAGAACTTTGCACAAGC
ATTCGGTTCTCAGTTTAAGTCGGGCAGCAGGGTGCCAATGACCTTTATCACTAACTCTAATGGAGAAGTG
GACCATAGAGTAAGGACTTCAGTGTCAGATTTCTCAGGGTATACAAATATGATGTCTGATGTAAGTGAGC
CATGTAGTACAAGAGTAAAGACACCCACCAG
Notes:The probe template was PCR amplified from IMAGE:642898 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:642898 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6637 same embryo
 EMAGE:6636 same embryo
 EMAGE:6638 same embryo
 EMAGE:6640 same embryo
 EMAGE:6635 same embryo
 EurExpress:euxassay_007265 same experiment
 MGI:4829301 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS