EMAGE mouse gene expression spatial database

EMAGE:6732

Prg3 proteoglycan 3 ( MGI:1858200)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002351. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002351_01	euxassay_002351_02	euxassay_002351_03	euxassay_002351_04

euxassay_002351_05	euxassay_002351_06	euxassay_002351_07	euxassay_002351_08	euxassay_002351_09

euxassay_002351_10	euxassay_002351_11	euxassay_002351_12	euxassay_002351_13	euxassay_002351_14

euxassay_002351_15	euxassay_002351_16	euxassay_002351_17	euxassay_002351_18	euxassay_002351_19

euxassay_002351_20	euxassay_002351_21	euxassay_002351_22	euxassay_002351_23	euxassay_002351_24

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1920
Entity Detected:	Prg3, proteoglycan 3 ( MGI:1858200)
Sequence:	sense strand is shown >T1920 TGGCCTCGAGCCAGAATTCGGACGAGGCAAATTCAGAACTTGGCCAGGAAGATCAATCAGTCCATCGTCT GGATTGGAGGCATCCTCAGGGGATGGTTCTGGAAGAAGTTTTGCTGGATGGATGGGAGCTGCTGGGATTT TGGATACTGGGCCCCAGGGCAGCCTGGGAGTGGGGGAGGACACTGTGTGACTCTGTGTACCAAAGGGGGC CATTGGCGAAGAGCTTCATGCAAAAGTCACCTGCCTTTCATCTGCTCCTTCTAAGATGGAGCTAGATCCT ACTTGGAATCTCCTGCCTTCACAGCCCTGGCTGCTTATCCCCATCCTTTGGCATTCAATTCTGTCCCCAG TCCCTCCTGGTCATAAAGTCAGGCTTTCCACAGCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:634700 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:634700 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6733 same embryo
	EMAGE:6736 same embryo
	EMAGE:6734 same embryo
	EMAGE:6731 same embryo
	EMAGE:6735 same embryo
	EurExpress:euxassay_002351 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source