EMAGE mouse gene expression spatial database

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EMAGE:6745

Ruvbl2 RuvB-like protein 2 ( MGI:1342299)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002276. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002276_01	euxassay_002276_02	euxassay_002276_03	euxassay_002276_04

euxassay_002276_05	euxassay_002276_06	euxassay_002276_07	euxassay_002276_08	euxassay_002276_09

euxassay_002276_10	euxassay_002276_11	euxassay_002276_12	euxassay_002276_13	euxassay_002276_14

euxassay_002276_15	euxassay_002276_16	euxassay_002276_17	euxassay_002276_18	euxassay_002276_19

euxassay_002276_20	euxassay_002276_21	euxassay_002276_22	euxassay_002276_23	euxassay_002276_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6745_wholemount_strong.wlz

6745_wholemount_moderate.wlz

6745_wholemount_weak.wlz

6745_wholemount_possible.wlz

6745_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6745_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 24

axial muscle

moderate

regional

moderate expression: see section 05 06 07 08 09 10 21 22 23

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 11 17 18 19 20

orbito-sphenoid

strong

regional

strong expression: see section 01 02 05 19 moderate expression: see section 03 04 20 21 24

viscerocranium

strong

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3380
Entity Detected:	Ruvbl2, RuvB-like protein 2 ( MGI:1342299)
Sequence:	sense strand is shown >T3380 TGGCCTCGAGCCAGATTCGGAACGAGGGCAGCCACCACCAAAGTCCCTGAGATCCGAGATGTGACAAGAA TCGAGCGAATCGGCGCTCACTCCCACATCCGGGGGCTGGGACTGGATGATGCCCTGGAGCCAAGACAGGC TTCCCAGGGAATGGTGGGGCAGCTAGCTGCCCGGCGTGCAGCTGGCGTGGTGCTGGAGATGATCCGAGAA GGGAAGATTGCAGGGCGGGCCGTCCTCATTGCAGGCCAGCCAGGCACTGGGAAGACAGCCATTGCCATGG GCATGGCCCAGGCACTGGGCCCCGACACACCATTCACAGCCATCGCAGGCAGTGAGATCTTTTCTCTGGA GATGAGCAAGACTGAGGCTCTAACGCAAGCCTTCCGCCGTTCCATTGGTGTCCGGATCAAGGAGGAGACA GAGATCATTGAAGGGGAGGTGGTAGAGATCCAGATTGACCGGCCAGCCACAGGCACGGGATCCAAGGTGG GCAAACTGACCCTCAAGACCACGGAGATGGAGACCATCTACGACCTGGGTACCAAG
Notes:	The probe template was PCR amplified from IMAGE:2938212 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2938212 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6747 same embryo
	EMAGE:6748 same embryo
	EMAGE:6744 same embryo
	EMAGE:6746 same embryo
	EMAGE:6743 same embryo
	EurExpress:euxassay_002276 same experiment
	MGI:4827840 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source