EMAGE mouse gene expression spatial database

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EMAGE:6763

Pacs1 phosphofurin acidic cluster sorting protein 1 ( MGI:1277113)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007319. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007319_01	euxassay_007319_02	euxassay_007319_03	euxassay_007319_04

euxassay_007319_05	euxassay_007319_06	euxassay_007319_07	euxassay_007319_08	euxassay_007319_09

euxassay_007319_10	euxassay_007319_11	euxassay_007319_12	euxassay_007319_13	euxassay_007319_14

euxassay_007319_15	euxassay_007319_16	euxassay_007319_17	euxassay_007319_18	euxassay_007319_19

euxassay_007319_20	euxassay_007319_21	euxassay_007319_22	euxassay_007319_23	euxassay_007319_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6763_wholemount_strong.wlz

6763_wholemount_moderate.wlz

6763_wholemount_weak.wlz

6763_wholemount_possible.wlz

6763_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6763_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 05

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 09 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 weak expression: see section 21

vagus x ganglion

moderate

regional

moderate expression: see section 09 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 09 18 19 20 weak expression: see section 21

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1872
Entity Detected:	Pacs1, phosphofurin acidic cluster sorting protein 1 ( MGI:1277113)
Sequence:	sense strand is shown >T1872 TGGCCTCGAGGCCAGATTCGGACGAGGCTGCCTCCAGCCTCCACCTAAGATTGTTCTGTGGCTTCATGAG GTAGGAGACAGCAAACCCACCCCTCCACATACATTATCTGTGACTCTTACCCAGTCATATGTCTCCCTTT TCCTGTGTCAGCTTGCGTCCCCATCCCTGCTTTGTCTTCCCACTTCTGTCATGGCAGGGCAGCAGAAAGA GGCCCATCTCCACCAGGTCTCCCTCCAGCAGCCTCCTTCCCTCTCTCAGCCATGGATGGGTCCCAGACCT ATCTCAGCCTCCTCATCAGTGTCTTCATGAGAGGCTGAGGACACGTGACAGAGCTAGCCCAGCCTCCATG CCCTCTGGCACTTCAGAGCTTCTCTGTTGCCCTCTCACTGCTTCCTAGCAGTGTGGCACAGAACTGCACT CAGAACATAGTTTTGGCATTCTTCACATCCAAGACTCCAGCCCTTCCTGCTGCCCAGCCCTACTGTCCAG AGTGTCTCTTGGGCCTGAGAATAAGTGGGGTGGGAAGACTGGGGCCATGTT
Notes:	The probe template was PCR amplified from IMAGE:596587 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:596587 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6764 same embryo
	EMAGE:6765 same embryo
	EMAGE:6767 same embryo
	EMAGE:6766 same embryo
	EurExpress:euxassay_007319 same experiment
	MGI:4827021 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source