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EMAGE:6802

Hsd3b1 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 ( MGI:96233)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6802 EMAGE:6802 EMAGE:6802 EMAGE:6802 EMAGE:6802
"Pseudo-wholemount" of euxassay_007387. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007387_01 euxassay_007387_02 euxassay_007387_03 euxassay_007387_04
EMAGE:6802 EMAGE:6802 EMAGE:6802 EMAGE:6802 EMAGE:6802
euxassay_007387_05 euxassay_007387_06 euxassay_007387_07 euxassay_007387_08 euxassay_007387_09
EMAGE:6802 EMAGE:6802 EMAGE:6802 EMAGE:6802 EMAGE:6802
euxassay_007387_10 euxassay_007387_11 euxassay_007387_12 euxassay_007387_13 euxassay_007387_14
EMAGE:6802 EMAGE:6802 EMAGE:6802 EMAGE:6802 EMAGE:6802
euxassay_007387_15 euxassay_007387_16 euxassay_007387_17 euxassay_007387_18 euxassay_007387_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6802Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6802_wholemount_strong.wlz
6802_wholemount_moderate.wlz
6802_wholemount_weak.wlz
6802_wholemount_possible.wlz
6802_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6802_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
adrenal medulla
strong strong
regionalstrong expression: see section 04 05 06 07 13 14 15 16
kidney calyx
strong strong
regionalstrong expression: see section 05 06 07 16 17 moderate expression: see section 15
ovary
moderate moderate
regionalmoderate expression: see section 03 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1836
Entity Detected:Hsd3b1, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 ( MGI:96233)
Sequence:sense strand is shown

>T1836
AATTCGGATCCATGGGANATCAGCATCCAGACACTCTCATCTGACTTTTAACAATTTAACAGCCCTCCTA
AGGGTTACCCTATATCATACCAGCTCCCACTGTCATCTGTTTCCTGTGTTGACCATGGCTGGATGGAGCT
GCCTGGTGACAGGAGCAGGAGGGTTTGTGGGCCAGAGGATCATCAAGATGTTGGTGCAGGAGAAAGAACT
GCAGGAGGTCAGAGCTCTGGACAAAGTATTCCGACCAGAAACCAAGGAGGAATTCTCCAAGCTGCAGACA
AAGACCAAGGTGACAGTGTTGGAAGGAGACATTCTGGATGCCCAGTGCCTGAGGAGAGCCTGCCAGGGCA
TCTCTGTTGTCATCCACACTGCTGCTGTCATTGATGTCACAGGTGTCATTCCCAGGCAGACCATCCTAGA
TGTCAATCTGAAAGGTACCCAGAACCTATTGGAGGCCTGTGTTCAAGCAAGTGTGCCAGCCTTCATCTTC
TGCAGCTCAGTTGATGTTGCAGGGCCCAACTCGTACAAGAAGATCGTCCTGAA
Notes:The probe template was PCR amplified from IMAGE:580893 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:580893 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6806 same embryo
 EMAGE:6804 same embryo
 EMAGE:6803 same embryo
 EMAGE:6807 same embryo
 EMAGE:6805 same embryo
 EurExpress:euxassay_007387 same experiment
 MGI:4825465 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS