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EMAGE:6810

Bach2 BTB and CNC homology 2 ( MGI:894679)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6810 EMAGE:6810 EMAGE:6810 EMAGE:6810 EMAGE:6810
"Pseudo-wholemount" of euxassay_002436. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002436_01 euxassay_002436_02 euxassay_002436_03 euxassay_002436_04
EMAGE:6810 EMAGE:6810 EMAGE:6810 EMAGE:6810 EMAGE:6810
euxassay_002436_05 euxassay_002436_06 euxassay_002436_07 euxassay_002436_08 euxassay_002436_09
EMAGE:6810 EMAGE:6810 EMAGE:6810 EMAGE:6810 EMAGE:6810
euxassay_002436_10 euxassay_002436_11 euxassay_002436_12 euxassay_002436_13 euxassay_002436_14
EMAGE:6810 EMAGE:6810 EMAGE:6810 EMAGE:6810 EMAGE:6810
euxassay_002436_15 euxassay_002436_16 euxassay_002436_17 euxassay_002436_18 euxassay_002436_19
EMAGE:6810 EMAGE:6810 EMAGE:6810 EMAGE:6810 EMAGE:6810
euxassay_002436_20 euxassay_002436_21 euxassay_002436_22 euxassay_002436_23 euxassay_002436_24
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euxassay_002436_25 euxassay_002436_26 euxassay_002436_27

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6810Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6810_wholemount_strong.wlz
6810_wholemount_moderate.wlz
6810_wholemount_weak.wlz
6810_wholemount_possible.wlz
6810_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6810_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
vibrissa
strong strong
regionalstrong expression: see section 06 07 09 18 19 weak expression: see section 20 21
cerebral cortex marginal layer
strong strong
regionalstrong expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 moderate expression: see section 06 07 08 09 10 11
telencephalon mantle layer
strong strong
regionalstrong expression: see section 21 22 23 24 25 moderate expression: see section 05 06 07
telencephalon marginal layer
moderate moderate
regionalmoderate expression: see section 08
olfactory cortex marginal layer
strong strong
regionalstrong expression: see section 15 moderate expression: see section 11 12 weak expression: see section 16 17 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 06 weak expression: see section 07 08 09 10 19 21 22 23 24
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 11 12 weak expression: see section 13 14 15 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1953
Entity Detected:Bach2, BTB and CNC homology 2 ( MGI:894679)
Sequence:sense strand is shown

>T1953
GGCCTCNAGGCCAGATTCGGCACGAGGGTTTTTTTTTTCTCAGTGGCCCATGTCACAAACCCTATCTCAG
GAATTTCTTCTGAATGTTCAATTTTTTTCATTGAAGACAGCTTCTATACACATCAAAGTTTTATAGCTAT
ACTGTGTACATAGTATATATAATATATATGAAATATATATCCATATGCAAAAAGTCCTGCATGCCTCAAT
TTTCTCATCCCTGAAACTGGAAGCTTCACTTATCATTTACAAACAGGTTCCAACATTCCTCTTTTTGTGT
CTGGTGCCAGAACTGGTTTGGAAGCTGTTAACATGGCTGTTTTGCTTGCTGCACAATTCGGTTTCCATCT
GTGCTTATTTACAGACAAAATTCAATGTTGGGAGATGCTTCTCAAGGTTCAATCTCAGACCTTTTACTTT
CGTTGGTTTGGTTTTGGTGCCGCCGAACGGTGTCAGGTGAGCACCGTGAGTCCGCTCTTCTCCCCTTGTG
TTTTCCCCTCGTCTCTGCGGATACTGTACAGCAATGGTCAACTTTGCCACTTGCACTGAGTTTTGAGTCA
AACCTATTT
Notes:The probe template was PCR amplified from IMAGE:640200 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:640200 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6809 same embryo
 EMAGE:6808 same embryo
 EurExpress:euxassay_002436 same experiment
 MGI:4823382 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS