EMAGE mouse gene expression spatial database

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EMAGE:6848

Ces2a carboxylesterase 2A ( MGI:2142491)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002532. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002532_01	euxassay_002532_02	euxassay_002532_03	euxassay_002532_04

euxassay_002532_05	euxassay_002532_06	euxassay_002532_07	euxassay_002532_08	euxassay_002532_09

euxassay_002532_10	euxassay_002532_11	euxassay_002532_12	euxassay_002532_13	euxassay_002532_14

euxassay_002532_15	euxassay_002532_16	euxassay_002532_17	euxassay_002532_18	euxassay_002532_19

euxassay_002532_20	euxassay_002532_21	euxassay_002532_22	euxassay_002532_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6848_wholemount_strong.wlz

6848_wholemount_moderate.wlz

6848_wholemount_weak.wlz

6848_wholemount_possible.wlz

6848_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6848_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5046
Entity Detected:	Ces2a, carboxylesterase 2A ( MGI:2142491)
Sequence:	sense strand is shown >T5046 CCACGCGTCCGCACTCTCATGTGCAGCAGCACAGGTTTAGGCCTGAGATTCAACCATGCCATTGGCTAGA CTTCCTGGTTGGCTGTGTGTTGTTGCCTGTGGGCTCCTGCTTCTCCTCCAGCATGTGCATGGCCAGGACT CAGCCAGCCCCATCAGGAACACCCACAGGGGTCAGGTGAGAGGCAGCTTTGTCCACGTGAAGGACACTAA ATCTGGTGTCCATGCCTTCCTGGGAATCCCCTTTGCCAAGCCTCCTGTAGGACTACTGCGCTTTGCACCT CCTGAGGACCCTGAGCCATGGAGTGGTGTGAGAGATGGGACCTCACAGCCGGCCATGTGTCTGCAGCCTG ATATAATGAATTTAGAGGATGCGAAGGAAATGAATCTGATCCTGCCTCCCATCTCTATGTCCGAGGACTG CCTGTATCTCAACATCTACACACCAACCCACGCCCAGGAGGGCTCTAACCTGCCTGTGATGGTATGGATC CACGGTGGTGGACTGGTTGTAGGATCAGCTTCCATGAATGATGTATCTAAACTGGCAGCCACTGAGGAAA TAGTAATTGTCGCCATCCAGTATCGCTTGGGGGTCCTTGGCTTCTTCAGCACTGGAGACCAGCATGCCAG AGGCAACTGGGGATACCTGGACCAAGTGGCTGCCTTACGCTGGGTGCAGAAGAATATTGCTTACTTTGGA GGCAACCGTGACAGGGTCACTATATTTGGAGTCTCAGCAGGAGGTACAAGTGTGTCTTCTCATATTCTGT CCCCAATGTCCAAAGGACTCTTTCATGGAGCCATTATGCAGAGTGGAGTGGCCCTGCTGCCTGACCTCAT CTCTGACACTTCTGAGGTGGTCTACAAGACAGTAGCCAACCTATCTGGTTGTGAAGCCACAGACTCAGAA GCCCTGATCCACTGCCTGAGAGCAAAGAGTAAACAAGAGATCCTGGCTATTAACCAGGTCTTCAAGATGA TCCCTGCTGTGGTGGATGGGGAGTTCCTACCCAAGCATCCTCAGGAGCTGCTGACCTCTATGGATTTTCA CCCTGTCCCCAGCATCATTGGTGTCAACACAGATGAATGTGGCTGGGGAGTCCCCATGTTCATGGGCCTT GACCATATCATAAAGAACATAACCAGAGAGACCCTGCCAGCTGTTCTGAAGAACACAGCAGCACGGATGA TGTTGCCTCCAGAATGTAGTCACCTGCTAGTGGAGGAGTATATGGGGGACACTGAGGACCCAGAGACCCT GCAAGCACAGTTCAGAGAGATGCTGGGGGACTTCATGTTTGTGATCCCTGCTCTCCAAGTGGCACATTTC CAGCGTTCCCAGGCTCCTGTCTACTTCTATGAGTTCCAGCATCTGTCCAGCTTCATCAAGCATGTCAGGC CATCACATGTGAAGGCAGACCATGGTGATGACGTTGCCTTTGTCTTTGGCTCCTATTTGTGGGACATGAA TTTGGACCTCACTGAGGAGGAGGAGCTGCTAAAGAGGATGATGATGAAGTACTGGGCCAATTTCGCAAGA AATGGGAACCCCAACAGTGAGGGTCTACCCTCCTGGCCTGTGTTGGACCATGATGAGCAGTACCTGCAGC TGGACACCCAGCCTGCTGTGGGTCGAGCCCTGAAGGCCAGAAGGCTGCAGTTCTGGACCAAGACTCTGCC CCAGAAGATCCAGGAGCTCAAGGGTTCTCAGGACAAGCATGCAGAGCTGTAGCGTCCTGTGTCAGGGAAG GTGTGTGAGATTAAAAGCAGATGGTGTAATTCTGAGGTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5309880 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5309880 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6845 same embryo
	EMAGE:6846 same embryo
	EMAGE:6843 same embryo
	EMAGE:6844 same embryo
	EMAGE:6842 same embryo
	EMAGE:6847 same embryo
	EurExpress:euxassay_002532 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source