EMAGE mouse gene expression spatial database

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EMAGE:6862

Mlx MAX-like protein X ( MGI:108398)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002505. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002505_01	euxassay_002505_02	euxassay_002505_03	euxassay_002505_04

euxassay_002505_05	euxassay_002505_06	euxassay_002505_07	euxassay_002505_08	euxassay_002505_09

euxassay_002505_10	euxassay_002505_11	euxassay_002505_12	euxassay_002505_13	euxassay_002505_14

euxassay_002505_15	euxassay_002505_16	euxassay_002505_17	euxassay_002505_18	euxassay_002505_19

euxassay_002505_20	euxassay_002505_21	euxassay_002505_22	euxassay_002505_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6862_wholemount_strong.wlz

6862_wholemount_moderate.wlz

6862_wholemount_weak.wlz

6862_wholemount_possible.wlz

6862_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6862_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5025
Entity Detected:	Mlx, MAX-like protein X ( MGI:108398)
Sequence:	sense strand is shown >T5025 GTTCGGTAGGTTCACGATGACGGAGCCGGGCGCCTCTCCGGAGGACCCTTGGGTCAAGGCAAGCTTCGCG GACGCGCACGCCGGCGAGGGGAGGGCGGGTCGGGCTCGTGCACGTAGGGGGTCCGGAAGACGCGGGGCTC CCCAACTATCCCCAGAGTCTCCCCTGCTGTCCGGGGCCCGGGGCTGCAGAGAAGACAGCTCTCACCCGGC CTGTGCCAAGGTCGAGTATGCCTACAGTGACAACAGCCTGGATCCTGGGCTTTTTGTAGAAAGCACCCAC AAGGGGAGTGTAGTGTCCAGAGCTAATAGCATCGGCTCCACCAGTGCCTCTTCTGTCCCCAACACAGATG AGGAGGACAGTGATTATCAGCAGGAGTCCTACAAGGAGTCCTACAAAGACCGGAGGCGGCGTGCGCACAC TCAGGCTGAACAGAAGAGAAGGGATGCTATTAAGAGAGGCTATGATGACCTTCAGACCATTGTCCCTACC TGCCAGCAGCAGGATTTCTCCATTGGCTCCCAAAAACTCAGCAAAGCCATCGTTCTACAGAAGACCATTG ACTACATCCAGTTTTTACACAAGGAGAAGAAAAAGCAGGAGGAAGAAGTATCCACACTGCGCAAGGACGT CACAGCCTTGAAGATAATGAAAGTGAACTATGAGCAAATCGTGAAGGCACATCAGGACAACCCTCATGAG GGAGAGGACCAGGTCTCTGACCAGGTCAAGTTCAACGTCTTTCAAGGCATCATGGACTCCCTCTTCCAGT CTTTCAACGCCTCTATCTCTGTGGCCAGCTTCCAGGAGCTCTCAGCTTGTGTCTTCAGCTGGATTGAGGA ACACTGTGAGCCACAGACTCTACGAGAGATTGTGATCGGTGTCCTTCATCAGTTGAAAAACCAACTCTAC TGATTGCCCATGGAAGCCTGCAGAGCAGCCAACAAGAGGCCTTTGAGAGTGTGGCCATGGAACTAACTGC TGGGCCTATGAGACTGGCCTGCAACACCTCACACCGGTCAGCTGGTTTCTACTTGGTGTTTGGTTTTCCC AACCCCACTTCAGCTTCGGTGGGGTTGAGTGTTTTGTGAAAGCTTCTGAATAATTTATTATATTGTCCAC AATGTTGAACCTACCCAACCTTCCCCTTTCTAGGAAAAGCCTCTAGGACAAGAGTCTGTTCAGGGAGCCT CACAGAACCCTGAGCCCCTCAGCCTTTTCTCTACCCACTGGGTTCCTGCTCACAATCTATATACATAATT TGGAAATCGTTTGCCTCTGCTTGGTCTCTTGGGCAACACTTGGCTCAAGTTTGGGTATTTTGGCAGTTCT TAAGTTAGAAAAAAGGAAAAGAAATAGCCCTATGGCCATGAAGGGTAAAAGGCCACCTTGTGCCTAGGCT AGGGCTGTGTGGTCAATACTAAGTAAGGCCAGGGTATTCTAGCCAGCTCAGGGTAGGGCTGCCTGACCCT GCTACCCTCATGTTCTGCTGCCCACTTCAGCTGCTGTAGGTGCTACAGGAAACAGCGTCCCATCAGCTCT TGCCCTGTCTTCCCAAAGGCAAAGGCAGCATTACATATTTGAATTTATCTTCTAAATACCCACAGTGCAT TTTTGTTATATGCAGTTTCCCAAACTTCTTAGCAGACCTCCAAAGCAGCCCCAGCCTCTTCTGCATAATG TGTGTATCTGCCCCACCAGTGTGTTGTCTCCATTTCTCCTGGGGCCTGGGGATAGAGTTAGGTGGGACAG GAGATCCTCAAGTCTTCTGGGGGTAGGCCAGGGCGATCCCTTGGGAGTCTCAAATGTTGGTGTATGGCAC CCTTTCTGTACTCTTATCCCATGTTTCCTACTGCCTTCCCCAGGTTTGATATCTGGGAGGTTTGATCATC CAGAGGAAATTAAGTATTTTTATATCAAATTATATTACAATAAATATTACAAATACTTTCTTTTAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5252695 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5252695 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6861 same embryo
	EMAGE:6858 same embryo
	EMAGE:6863 same embryo
	EMAGE:6860 same embryo
	EMAGE:6859 same embryo
	EurExpress:euxassay_002505 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source