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EMAGE:6895

Rgnef Rho-guanine nucleotide exchange factor ( MGI:1346016)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6895 EMAGE:6895 EMAGE:6895 EMAGE:6895 EMAGE:6895
"Pseudo-wholemount" of euxassay_002393. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002393_01 euxassay_002393_02 euxassay_002393_03 euxassay_002393_04
EMAGE:6895 EMAGE:6895 EMAGE:6895 EMAGE:6895 EMAGE:6895
euxassay_002393_05 euxassay_002393_06 euxassay_002393_07 euxassay_002393_08 euxassay_002393_09
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euxassay_002393_10 euxassay_002393_11 euxassay_002393_12 euxassay_002393_13 euxassay_002393_14
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euxassay_002393_15 euxassay_002393_16 euxassay_002393_17 euxassay_002393_18 euxassay_002393_19
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euxassay_002393_20 euxassay_002393_21 euxassay_002393_22 euxassay_002393_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6895Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6895_wholemount_strong.wlz
6895_wholemount_moderate.wlz
6895_wholemount_weak.wlz
6895_wholemount_possible.wlz
6895_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6895_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 08 14 15
cervical ganglion
strong strong
regionalstrong expression: see section 07 08 15 16
thoracic ganglion
strong strong
regionalstrong expression: see section 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3272
Entity Detected:Rgnef, Rho-guanine nucleotide exchange factor ( MGI:1346016)
Sequence:sense strand is shown

>T3272
TGGCCTCGAGCAGATTCGGACGAGGTGACCATCCAGGACAGTCACATTGAGATCCACAAACTGGTTCTAC
AGCAGCGGGAGAGCTTGGCCCCTAGCCACTCCTTCCGAGGTGGCCCCTTGCAAGACCAGGAGAAATCCCG
CTACCTGGAAAAGCAGCGTGAAGAGCTGGCCAACATCCACAAGCTTCAACACCAGTTCCAGCAGGAGCAG
CGGCGCTGGCACCGCACGTGCGACCAGCAACAGCGCGAGCAGGAAGCCCAAGAGAGCTGGCTGCAGGCGC
GGGAACGTGAGTGCCAGTCCCAGGAGGAATTGTTGCTGCGCCACCGTAGCGAGCTGGACCACCAACTCCA
GGAGTACCAGCAGAGCCTTGAGCGGCTACGGGAGGGCCAGCGGATGGTGGAGAGGGAGAGACAGAAGATG
CGTGTCCAGCAGGGTCTGCTGGGCCACTGCAAGCATAGTCGCCAGCGGAGCCTCCCAGCCGTCTTCTCTC
CAGGGAGCAAGGAGGTAACAGAACTTAACCGAGCTGAGAGTTTGTGTCATGAAAAT
Notes:The probe template was PCR amplified from IMAGE:2780140 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2780140 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6898 same embryo
 EMAGE:6894 same embryo
 EMAGE:6899 same embryo
 EMAGE:6897 same embryo
 EMAGE:6896 same embryo
 EurExpress:euxassay_002393 same experiment
 MGI:4827716 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS