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EMAGE:6906

P2rx1 purinergic receptor P2X, ligand-gated ion channel, 1 ( MGI:1098235)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6906 EMAGE:6906 EMAGE:6906 EMAGE:6906 EMAGE:6906
"Pseudo-wholemount" of euxassay_002455. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002455_01 euxassay_002455_02 euxassay_002455_03 euxassay_002455_04
EMAGE:6906 EMAGE:6906 EMAGE:6906 EMAGE:6906 EMAGE:6906
euxassay_002455_05 euxassay_002455_06 euxassay_002455_07 euxassay_002455_08 euxassay_002455_09
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euxassay_002455_10 euxassay_002455_11 euxassay_002455_12 euxassay_002455_13 euxassay_002455_14
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euxassay_002455_15 euxassay_002455_16 euxassay_002455_17 euxassay_002455_18 euxassay_002455_19
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euxassay_002455_20 euxassay_002455_21 euxassay_002455_22 euxassay_002455_23 euxassay_002455_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6906Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6906_wholemount_strong.wlz
6906_wholemount_moderate.wlz
6906_wholemount_weak.wlz
6906_wholemount_possible.wlz
6906_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6906_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
foregut-midgut junction
strong strong
regionalstrong expression: see section 13 14
pancreas
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17
midgut
strong strong
regionalstrong expression: see section 10 11 12
liver lobe
strong strong
single cellstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1813
Entity Detected:P2rx1, purinergic receptor P2X, ligand-gated ion channel, 1 ( MGI:1098235)
Sequence:sense strand is shown

>T1813
TGGCCTCGAGCAGATTCGGACGAGGCCCTGAGATGGAAGCCCAGAACGGGGTGGGAACTCAGGGGTTCCT
GGCTCCCTACAGGTATTCGCACAGGCAACTGTGTGCCCTTCAATGGCACTGTGAAGACGTGTGAGATCTT
TGGCTGGTGTCCTGTAGAGGTGGATGACAAGATCCCAAGCCCTGCTCTTCTTCATGAGGCTGAGAACTTC
ACCCTCTTCATCAAAAACAGCATCAGCTTTCCACGCTTCAAGGCGCAACCTGGTGGAGGAGGTGAATGGT
ACCTACATGAAGAAATGCCTCTACCACAAGATTCTACACCCGCTGTGCCCAGTCTTCAGCCTTGGCTATG
TGGTGCGAGAGTCGGGCCAGGACTTCCGAAGCCTTGCTGAGAAGGGCGGGGTGGTTGGTATCACCATCGA
CTGGGAGTGTGACCTGGACTGGCATGTACGGCACTGCAAACCCATCTACCAGTTCCATGGACTGTACGGG
GAGAAGAACCTGTCTCCAGGCTTCAACTTCAGATTTGCCAGGCACTTTGTGCAGAATGGGACAAACCGTC
GTCA
Notes:The probe template was PCR amplified from IMAGE:576538 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:576538 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6905 same embryo
 EMAGE:6910 same embryo
 EMAGE:6909 same embryo
 EMAGE:6907 same embryo
 EMAGE:6908 same embryo
 EurExpress:euxassay_002455 same experiment
 MGI:4827011 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS