EMAGE mouse gene expression spatial database

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EMAGE:6966

Rinl Ras and Rab interactor-like ( MGI:2444024)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002473. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002473_01	euxassay_002473_02	euxassay_002473_03	euxassay_002473_04

euxassay_002473_05	euxassay_002473_06	euxassay_002473_07	euxassay_002473_08	euxassay_002473_09

euxassay_002473_10	euxassay_002473_11	euxassay_002473_12	euxassay_002473_13	euxassay_002473_14

euxassay_002473_15	euxassay_002473_16	euxassay_002473_17	euxassay_002473_18	euxassay_002473_19

euxassay_002473_20	euxassay_002473_21	euxassay_002473_22	euxassay_002473_23	euxassay_002473_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6966_wholemount_strong.wlz

6966_wholemount_moderate.wlz

6966_wholemount_weak.wlz

6966_wholemount_possible.wlz

6966_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6966_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5118
Entity Detected:	Rinl, Ras and Rab interactor-like ( MGI:2444024)
Sequence:	sense strand is shown >T5118 CTTTCACTTCCTGCTGCTCTGGCTCCCCTCGGAACAGCTGAGCAGCCTTGAGTCTTGTCTTCCTCCATGC CAGAGGCCAGAGCATGGCTCACTGGTGCCTGGCAGCATGAGGCTAGCCCAGCCGGACATGGTGTCTGCAG CCCCTACTGAAGTGGACAGGCTGGTTTGGCCACTAGCGGATGGAGCAGACAAGAGCCCACTAGGGGTCCT CAGCACCACAGAGCCACTTCTTCGACTGCAGAGGACACAGAGAGTGTGGGAGGTCCCCGAGCTGGATGCT CAGTATGCCAAGGCCTTTCTAGAGCTGTGGCCACTGGGGAGCTTCCTGGTCATCGGACATGAGCCTGGCC AGGTCCTGATGTTGAAGGCAGGACCTTCTTCAGGAGATATCAACACCTATCAAATTCAGAGGTTTCCTGG AGGTGTGTCCCTGGAATCCTCCAACCTCTGCATGCCCGACTGTCCCCATCTCCTGGCCTTTCTGTCAGCC AGCAGGGACGTTTTGCCAAGGACCCTGCTCTTGCCCACGCCTACTGTGGGGGCTGGAGATAATCACTCAG ATCCCCATCGGCTTGGCTGCATCCAGGTGGACACCTCAGGGAGGGTGCTGTCTGTAGTGAACCAGCTCTA CCTGGAGACACATGGAGGCTGGGGAACAGAGACTCCACAGCAAACAGAGCCAGAGACTGGACAGAAATAC AGTCTAGCCCCCAGGAAGCCCACCCCACACCGGGTCTCCTGGGTGGAAGACCCTTTGAGACCGGAAGCTC ACCATACTGGACAGGAAGTGCATCATCCTGGAGCAGACGCTCACTCCCTTGGGTCGGAAGTGCACTTTTC CTGTCCTGCTCTAGAAGAGGAGGAAGTGAACAACGACTGCTACAAAGATGAAGACGAGGAAGGTTGCGAG GATATGCTCACTGCCCACATCCGTGCTCTGGCCAGGACCCGGAGCAGCTATGTAGCCAGGCAGTACCGAT GCCTTCGGGCACGCCTCATCTCAGATTCTGGAAATCCCTACAGCCCAGGGGACCCGGCCACAGAGCTGCT TCAGGATGTGCGCCAGCTCCTCACTGACCTCCAGAATTACCTGGCAAAAGACCCCGACGTCAGAGCTGTC TTTGGGAACAGGGGACCTAGCATCCTTCGGGAAGAAGATCTTGGCCCCGCTGTGGAGGTGGCCCTGTGTC GAGCGGTTCTAGAGCCCCTGAAGCCCGCCCTGTGGACCAAACTCCGAACTCTCAGAGCCCAGGAACTACG ACGACTGCGCAGGCGCCAAATAGCCTTGAGGGTAGGAGCGGGGCCTGAGGGACAGAGCCCGGCCTTGCGG AACCGGAACCGTATCCACGCACGCCTTGCTCACTTGCATGCGGCGTGCGCCCCGCGCCGCAAGGTGGCGC TGTTGCTGGCGGTGTGCAGCGACGTCTACGCGGGCCTAGGCGGTGGCGAGAATAAAGAGCCCCTGGGGGC TGACGCCTTTTTACCTGCCCTGACCGAGGAGCTGATCTGGAGTCCACATATTGGGGAAACACAGCTGGAT GTGGAGTTTCTTATGGAGCTCTTGGATCCAGGGGAGTTGCGAGGAGAAGCTGGGTATTATCTGACCACGT GGTTTGGGGCTCTCTACCACATTGCTCACTACCAGCCAGACACTGGCCGTGCACCCCAGGGGCTCAGCTC CGAGGCCCGGGCCTCCCTGCGCCAGTGGCATCGAAGACGGACACTGCACCAGCAAGCCCAGCCCACAGCC CAGGCCAACCAGCCTTTTGAGGAGCCATGGGCAATAGGGGACCCATGAAAACTGGAGTTCTCAAACCCCA GTCACTTCCGAACCTTTTGACCCACTCCTCTGCTAAGCAAGATTCTGGAGGCATCTGGGTCAGCATGGAG GGCAGGGCAGGAAAGGAGACGTGCCTCCTGCAATGTCTTACTTCCTCCTCCTCAATTCACCTCCTTCGGG TCTGTCCAGATGTTACCTCAACGAGGCGCTCCCCAGCAAACAAAACCCACACCCAGACAGTACTACAACT ACAACCCTCTCGCCTGAATCTCTTTGGGGCAGGTAGAATAAAGGCACCGTCCCCAAAGGTGTCTTCATCC TGATCCTATCTGTGACTGTGACACTTAACTTGGTCAAGTGATCGTTACAAAAATGACAGCATGTCTGGAC CTGAGATGGGAGCTTATTCTGGATTCTTCTGCAGCTCATGTAGTTAGGAGGGTGGCAGAGGTACAGGGAC CATCATGAGCTTACGCCTCCAGCTATACAGCAGAATGAGGTGGAAAAAGTACTTGAGGCCAAGACCATGA GGACACTGTGGCACTGAAACATAGTAGGTGTTTAGGAACACCTATGTGCCTTGAAAACATATCACTGCCC TAATCTCTGTGACCGTATTTGGCAAAAGTGTTTGTGCAGCTATTATTATTATTTTTGTTGTTTTGGTTTT TCAAGACAGGGCTCCTCGGTGTAGCCCTGGCTGTCCTGGAACTTGCTGTGTAGACCTGGCTGGCCTCGAA CTCAGACATCAGGGATTAAAGGTGTGTGCCCCCACCACCCAGCTCGTTCAGCCATCATTGAAGATGATAA AATGAGGGACAGTGCCCCGGGGACTGTGTCTGTGACTCCAGGAGCACCCACGCCATGCGGGAGACAAGGA GAACGAACGGATGGGAACAAGAGAGACGGGAGATGCTGGGCTACTCCAGGAAAGCTTCTCCCCACTGCCA CCCCCCCTCTGAGAGCTGCAGAAGTCATGGGGCCCTGCTGTCAACCTGGTTTCTGTGAGATTAGTGATGT CAGAACTCTGACCTCCAGAATTGTGAGCAAATAAATTCCTGCCGTCTTAAGACACCAAGGTGGTAATAAT AATAATAATCAATAAAATTTAAAATGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5329601 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5329601 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6968 same embryo
	EMAGE:6964 same embryo
	EMAGE:6965 same embryo
	EMAGE:6963 same embryo
	EMAGE:6967 same embryo
	EurExpress:euxassay_002473 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source