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EMAGE:6980

Sf1 splicing factor 1 ( MGI:1095403)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6980 EMAGE:6980 EMAGE:6980 EMAGE:6980 EMAGE:6980
"Pseudo-wholemount" of euxassay_004510. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004510_01 euxassay_004510_02 euxassay_004510_03 euxassay_004510_04
EMAGE:6980 EMAGE:6980 EMAGE:6980 EMAGE:6980 EMAGE:6980
euxassay_004510_05 euxassay_004510_06 euxassay_004510_07 euxassay_004510_08 euxassay_004510_09
EMAGE:6980 EMAGE:6980 EMAGE:6980 EMAGE:6980 EMAGE:6980
euxassay_004510_10 euxassay_004510_11 euxassay_004510_12 euxassay_004510_13 euxassay_004510_14
EMAGE:6980 EMAGE:6980 EMAGE:6980 EMAGE:6980 EMAGE:6980
euxassay_004510_15 euxassay_004510_16 euxassay_004510_17 euxassay_004510_18 euxassay_004510_19
EMAGE:6980 EMAGE:6980 EMAGE:6980 EMAGE:6980 EMAGE:6980
euxassay_004510_20 euxassay_004510_21 euxassay_004510_22 euxassay_004510_23 euxassay_004510_24
EMAGE:6980 EMAGE:6980
euxassay_004510_25 euxassay_004510_26

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6980Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6980_wholemount_strong.wlz
6980_wholemount_moderate.wlz
6980_wholemount_weak.wlz
6980_wholemount_possible.wlz
6980_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6980_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6356
Entity Detected:Sf1, splicing factor 1 ( MGI:1095403)
Sequence:sense strand is shown

>T6356
AGTAATGATCCCCCAAGACGAATATCCAGAAATCAATTTTGTGGGGCTCCTAATTGGACCCAGAGGGAAC
ACCCTGAAGAACATTGAGAAGGAATGCAACGCCAAGATCATGATTCGGGGGAAAGGATCAGTGAAAGAAG
GGAAAGTTGGGCGTAAAGATGGTCAGATGTTGCCAGGCGAAGATGAGCCTCTTCATGCTCTAGTCACTGC
CAATACGATGGAGAATGTCAAAAAGGCAGTAGAACAGATCAGAAACATCCTGAAGCAGGGTATTGAGACC
CCAGAGGACCAGAACGATCTACGGAAAATGCAGCTTCGAGAGTTGGCTCGCTTGAATGGCACTCTACGGG
AAGATGATAATAGGATCTTAAGGCCCTGGCAGAGCTCAGAGACGCGCAGCATTACCAATACTACTGTGTG
TACCAAGTGTGGAGGGGCTGGTCACATTGCTTCTGATTGCAAATTTCAGAGGCCTGGCGACCCTCAGTCA
GCTCAGGATAAAGCACGGATGGATAAAGAATATTTGTCCCTTATGGCTGAGCTAGGGGAAGCTCCTGTCC
CTGCATCTGTGGGCTCTACCTCTGGGCCTGCCACCACGCCCCTGGCTAGTGCACCAAGACCTGCTGCTCC
TGCCAGCAACCCACCACCACCGTCTCTCATGTCTACAACTCA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TTTAAACCACCTGCAGATTAC; Reverse Primer - name:unspecified, sequence:CATCATACCTTTTCCTTGATG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6979 same embryo
 EMAGE:6976 same embryo
 EMAGE:6977 same embryo
 EMAGE:6975 same embryo
 EMAGE:6978 same embryo
 EurExpress:euxassay_004510 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS