EMAGE mouse gene expression spatial database

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EMAGE:6999

Neil3 nei like 3 (E. coli) ( MGI:2384588)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002454. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002454_01	euxassay_002454_02	euxassay_002454_03	euxassay_002454_04

euxassay_002454_05	euxassay_002454_06	euxassay_002454_07	euxassay_002454_08	euxassay_002454_09

euxassay_002454_10	euxassay_002454_11	euxassay_002454_12	euxassay_002454_13	euxassay_002454_14

euxassay_002454_15	euxassay_002454_16	euxassay_002454_17	euxassay_002454_18	euxassay_002454_19

euxassay_002454_20	euxassay_002454_21	euxassay_002454_22	euxassay_002454_23	euxassay_002454_24

euxassay_002454_25	euxassay_002454_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6999_wholemount_strong.wlz

6999_wholemount_moderate.wlz

6999_wholemount_weak.wlz

6999_wholemount_possible.wlz

6999_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6999_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1990
Entity Detected:	Neil3, nei like 3 (E. coli) ( MGI:2384588)
Sequence:	sense strand is shown >T1990 GGCCTCGANGCCAGATTCGGCACGAGGATGCGCAGACCTGTCCTTCCCGTTCTGCAGACATGGCAAGCGC TCCATTATGAAAACTGTGCTGAAGATTGGACCTAATAATGGGAAGAATTTTTTTGTATGTCCTTTGGAGA AAAAAAAGCAGTGTAATTTTTTCCAGTGGGCCGAAAATGGACCAGGAATGGAAATTGTTCCAGGATGCTA ATAGTTTTCTCTTTTTAAAGAATGCCCGTCTCATAAACTCATCAAACTCATAGCATTCAGTCCCCTTTGT TTAATAGAAAACTTATAGAACACCTGTTGTACATACACATTAAGAACCTGTAGTATTTGAGACATATTTT TTTTCACTTAACTATGTGATCTTCACAGTTTTTGTTGTAATAATGAACACACTATTTCACCAGAGGCACA GTCCATTCTCTTTGAAATGCATATTCAATATAGTTAATAATGATGTCAATAAAGTACTCTTAGAATGTTA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:719423 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:719423 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6996 same embryo
	EMAGE:6995 same embryo
	EMAGE:6997 same embryo
	EMAGE:6994 same embryo
	EMAGE:6998 same embryo
	EurExpress:euxassay_002454 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source