EMAGE mouse gene expression spatial database

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EMAGE:7003

Ncaph non-SMC condensin I complex, subunit H ( MGI:2444777)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002558. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002558_01	euxassay_002558_02	euxassay_002558_03	euxassay_002558_04

euxassay_002558_05	euxassay_002558_06	euxassay_002558_07	euxassay_002558_08	euxassay_002558_09

euxassay_002558_10	euxassay_002558_11	euxassay_002558_12	euxassay_002558_13	euxassay_002558_14

euxassay_002558_15	euxassay_002558_16	euxassay_002558_17	euxassay_002558_18	euxassay_002558_19

euxassay_002558_20	euxassay_002558_21	euxassay_002558_22	euxassay_002558_23	euxassay_002558_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7003_wholemount_strong.wlz

7003_wholemount_moderate.wlz

7003_wholemount_weak.wlz

7003_wholemount_possible.wlz

7003_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7003_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 15 16 17

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 12 13 14 moderate expression: see section 07 08 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5164
Entity Detected:	Ncaph, non-SMC condensin I complex, subunit H ( MGI:2444777)
Sequence:	sense strand is shown >T5164 CCACGCGTCCGCGGACGCGTGGGCGCGATTTAAAACTTACTCCGGAGACGTGGAGAGCAAGATGAGAATT CCGCGCTCAGAAACAATGAATAGCTCTTTTCTGAAGGCCCGAGGACAACAGGATGTTCTTTCCTCACCTT TGGAACGGGTGCCCCCAGCATCCCGGCCCGGGAAGGCTCCACTTGGCACTCCTAAAACCCCAGTCCTCGA AGACTTTCCTCAGAATGACGATGAAAAGGAGCGGATGCAGCGGAGACGCTCCAGGGTCTTTGACCTGCAG TTCAGTACAGATTCGATTCATCTGGCCTCCCCTAACAGGAATATTGATGTTTCAACTACCATATCTAAGT TTACAAACACACAGATTACAGAACATTACTCTACCTGTATCAAGCTTTCTTCTGAAAATAAAATCACTAC TAAGAATGCTTTCGGCTTGCATTTGATTGATTTCATGTCAGAGATTCTGAAGCAGAAAGATGCAGAACCA ACCAACTTTAAAGTGGCTGCTGGCACCTTGGATGCCAGCACCAAGATCTATGCTGTCCGTGTGGATGCCG TCCATGCTGATGTATACAGAGTCCTTGGGGGGCTGGGCAAAGACACACCACCTCAAGGAGAGGAGAGCCA CAGTGGAGATGGAAGTACCCTTGAAACAGAAAGGACTAAAAAACCTGCAAAGCCAAAGAAGAAGCAGTCC TGCAAAACCATTGAACAGAATCTCAGCAATATCAATGTCTCGGAAGCCGATGGGAAGTGTGCGGTGGATC CTATGTTTCAGAAGACAGCAGCCTCCTTTGATGAATGCAGCACGACTGGGGTATTTCTCTCCACGCTGCA CTGCCAGGACTACAGAAGCGAGCTGCTCTTTCCTTCCGACATGCAGACCCTCTCCTCAGGAGAGCCCCTC GAGCTGCCGGATCTGGGTTTCGTAGACATGACAGATTTAGAAGCTTCCTTGCAGCAGTGTGTGGAAGACC GCCCGCTCTGCCCATCCCTGGCCGGGTTCCAGTTTACTAAATGGGACAGCGAAACACATAATGAGTCTGT GTCGGCCCTGGTGGACAAGTTTAAGAAGAATGATCAAGTATTTGACATCAATGCTGAGGCTGAAGACGAT GAAGAGGATGTCCCTGATGGGCCCCTGGTGGAGGATTTTGTTGACAATGATGAGCCAGACCTTTCTGCAG CTGGGGACCATGAAGAGTTCCGGAGCTGGAAGGAGCTCTGTCAGGTCCAGAGCAACCAGGAAGAAGTTAT TTCCCTCGAGGACAGAGACATCCAAGTCATGTGCTCCTTTTTATCCATGAAACCTGGGGAATATTCCTAT TTCAGTCCCCGGACCATGAAGATGTGGGCTGGCCCAGATCACTGGCGCTTCAGGCCTCGACCCAAGCAAG ATGCTACCTCCTGCACAGAGCACAAAAAGAAGAGTGCAAAGAAAGACTTTGAAATTAATTTTGATGATGA TATTGATTTTGATGCATATTTTCAGAAAACAAAGGCTGCTACCATTCTGACCAAGTCCACTTTGGAGAAC CAGAATTGGAAAGCCACCACTCTTCCCACAGATTTCCACTATGAAACTGATAATCTGATCCAGCTTCACC TCAAACCAGGCAAAAGGTCACTTAAGATGGACCAGGACCAGAAGGCTAAGACTGAGCACTATGAGGAAAT TGAAGACTATGATTACAACAACCCTAACGACACCTCCAACTATTGTCCTGGACTTCAGGCTGCTGACAGT GATTATGAAGAGGCAGACGATTTATTTGCGGATCCAGTCGGGACCCTTGACCTTGAGTCTGATCCAAAGA CAACACAAGAGAATGGTCACATTTCTCCTGAAAACCAAGGAGTAGATATCACAACTTACCAAGAGTTGAA CTTGGTGGCTGAGCCTCAAAAGGTGAATAAAATAGAAATTCATTACGCCAAGACTGCCAAAAAGATGGAC ATGAAGAAGCTGAAGCAGAGTATGTGGAGTCTGCTGACGAAGTTTTCTAGGAAGGAGGCTGACACAGAGG CAAACCACACTGAAAGTGGACAGGAAGGTGCCCCCGAGGAAGTGGCTGATGAGAAGAAACTCAGTGGGCT CACAAAGGACCTGCAGACAAGGCTGCCCCCGCTCATGGCTCAGAACCTCTCCATACCCCTGGCTTTTGCC TGTCTTCTGCACTTAGCAAATGAAAAGAATCTGAAGCTAGAAGGCACAGAGGATCTTTCTGATGTTCTGG TGATGCAAGGGGACTGAGTCCACTTTGAGACGTCTGCAGCGGGATGCCCATGGCTTCCTGGTGGCCGTGG AGAGGTGCTGGGCCTGTAGCGTGGCTATAGCCAACCACCACGGTGCTGGTTCTCGGGATGCCACCACGCT AGGAAGCTCCATGCTGTTGGAAGGTCTCACGTGGGTAACGTCCACCTAGGAGAGGGAAGCCCTTTAGTCT GTACAGACAGTCCGATCTTTATATCCATGTGCTCTGGTCACTTTCTGTGACCGTCCCACTTTTCATTCTG TACTGTCGAGAATCATTTCTCCATAGCCTCTTTCTGTCCTTAAAGAATTCCCAGATCTATGTACATAAAA TTTATCATTTTTAAACTTGTAAGTGTATTTAATACCCCAAAATAAAACTATTCTGGCAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:5346298 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5346298 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7002 same embryo
	EMAGE:7004 same embryo
	EMAGE:7001 same embryo
	EMAGE:7005 same embryo
	EMAGE:7000 same embryo
	EurExpress:euxassay_002558 same experiment
	MGI:4826613 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source