EMAGE mouse gene expression spatial database

EMAGE:7048

Pgc progastricsin (pepsinogen C) ( MGI:98909)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002331. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002331_01	euxassay_002331_02	euxassay_002331_03	euxassay_002331_04

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euxassay_002331_10	euxassay_002331_11	euxassay_002331_12	euxassay_002331_13	euxassay_002331_14

euxassay_002331_15	euxassay_002331_16	euxassay_002331_17	euxassay_002331_18	euxassay_002331_19

euxassay_002331_20	euxassay_002331_21	euxassay_002331_22	euxassay_002331_23	euxassay_002331_24

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3346
Entity Detected:	Pgc, progastricsin (pepsinogen C) ( MGI:98909)
Sequence:	sense strand is shown >T3346 AGNCAGATTCGGCACGAGGGNNCCCCAAANGAATGAACTTCTGCAGACCATAGGAGCCCAGGAAGGAGAG TATGGACAGTATTTTGTGAGCTGCGACAGCGTCAGTAGCCTGCCTACCCTCACTTTTGTCCTCAATGGTG TCCAGTTCCCCCTGTCACCCTCTTCCTACATCATCCAGGAGGAAGGCTCCTGCATGGTGGGTCTGGAGAG CCTCTCCCTGAACGCTGAGAGTGGCCAGCCCCTCTGGATCCTCGGGGATGTCTTCCTCAGGTCTTACTAT GCTGTCTTCGACATGGGCAATAACAGGGTGGGCCTTGCCCCTTCTGTCTAGACTTGACATCTAGACAAAC CCCCCCCCCACCCCCGTGCCCTTCCTTCTTCCCCTCAGCCTGTCCCATCTGCCCCGCCTCTCTGTATCCA GCTTTCCTTTTCTGGAAACCTAGACATTCCCTAATAATAAATAGGNCCTCCATATTGAAAAAAAAAAAAN AN
Notes:	The probe template was PCR amplified from IMAGE:317475 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:317475 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7050 same embryo
	EMAGE:7053 same embryo
	EMAGE:7049 same embryo
	EMAGE:7051 same embryo
	EMAGE:7052 same embryo
	EurExpress:euxassay_002331 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source