EMAGE mouse gene expression spatial database

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EMAGE:7080

Zfp788 zinc finger protein 788 ( MGI:1914857)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002470. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002470_01	euxassay_002470_02	euxassay_002470_03	euxassay_002470_04

euxassay_002470_05	euxassay_002470_06	euxassay_002470_07	euxassay_002470_08	euxassay_002470_09

euxassay_002470_10	euxassay_002470_11	euxassay_002470_12	euxassay_002470_13	euxassay_002470_14

euxassay_002470_15	euxassay_002470_16	euxassay_002470_17	euxassay_002470_18	euxassay_002470_19

euxassay_002470_20	euxassay_002470_21	euxassay_002470_22	euxassay_002470_23	euxassay_002470_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7080_wholemount_strong.wlz

7080_wholemount_moderate.wlz

7080_wholemount_weak.wlz

7080_wholemount_possible.wlz

7080_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7080_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5086
Entity Detected:	Zfp788, zinc finger protein 788 ( MGI:1914857)
Sequence:	sense strand is shown >T5086 CCACGCGTCCGCAAATTTTTGGAGTGCTTTAGGATTTAGTTGTCTTTGAGGATGTGGCTGTGAACTTCAC CGAGGAAGAGTGGGCTTTGCTGGATTCTTCTCAGAAGCATCTGTACAGAGATGTAATGCTGGAAATCTAC AATAACCTTGCTTGTGTCGGAAACAAATGGGAAGACCAAAGCATGGAAGATGAGCACACAAATCCTAGGA GAAGTCAAAGAATATGTAAGTTTGAGAGACTCTATGAGAGTACTGAAGGTGATCAATCTGGAGAAATCTT CACCAAGACACCAACTCTGACAAACATCTTCCCTGGTCTAAAATCTGAGCTTGATGAAAGTGGCGGGATG CAATATAAATGTGAACAGACTTGGAAAACAGACCTGAATTCTGTTACAAGTATTCCAATATATACACAAA ATCTCAGTATGAGTAGGTCTTATAACGTTTGTGCCACATCCTTTGGTTTTCCCAGCTCATTTGGAAGATA TCAAGAAACTCCTGTTGGAGAACAGCACTGTGAACAGAAGGTAAAAAACTGTCCTTCAGCTAAATCATTT TACATCCATGAAATGAATGACAATGCAGACATCGATAATGAACACAAACCATTTGGTAGAGCTCCGAGCT CTTTTGGTTCCTGTGAAAGTCGTGGAAGAATTAATACAAGGAAAAAGCCCTATGAATGTAGGCTGTGTAG TAAATCTTTCATGTATCCTTCTTTACTTCAAAAACATGAAATAACTCATTCCAATGAAAAACCCTACGGG TGCCTACAGTGTGGTAAAGCTTTCAGAAATTTCAGTTCTCTTCAAAAACATAAAATAGCTCATACTGAGG AAAAGCCTTATAAATGTCATTATTGTAATAAAGTGTTCATCTGTCAGGGATCACTTCAGTCACATATAAG AGTTCACACTGGGGAAAAGCCTTACGAATGTAAACAGTGTGGGAAAGCCTTTAGAGTTAACAGTTCCTTT TGGCGCCATAAAAAAGTTCATACTGGGGAAAAGCCCTATGAATGTAAACAATGTGGTAAGTCCTTTATTT ATCCTTCTCTACTTCAAACACATGAAAGAACTCACAGTGCAGAAAAGCCCTATAGCTGTAAACAATGTGG TAAGGCCTTTAAATCTCACAGTTCCCTTCGAGTCCACGAAAGAAACCATACTGGGAAAAAGCCCTATGAA TGTAAACAATGTGGCAGGTCCTTTATTTATCCCTGCTTACTCCAGATTCATGAACGAACTCACAGTGGTG TGAAACCCTACGGATGTAAACAGTGTGGGAAAGCGTTCAGGCGCCTCAGTGACCTTCGAGTCCATGAAAG AACTCATACCGGGGAAAAACCTTATGAATGTAAGCAGTGCGCTAAATCTTTTATTAATCGGTATTTGCTT AAAATGCATCAAAAGTCTCATACTGGCGAGAAACCCTATAAGTGTAAGATTTGCAGCAAAGCCTTCGTTT ATCCCTCCTTACTTAAATTGCATGAGAGATCTCACACTGGGGAAAAGCCCTATCCATGTAAACAATGTGA TAAAGCCTTCATTTTTCCCTCCTTACTTAAACTGCATGAAAGAACTCACACTGGGGAAAAACCCTATGCC TGTAAACAGTGTGGTGAAGCCTTCAAAAGTTACAACTCCCTTCAGAGACATAAAAGAATTCATACTGACG TAAAGGCTTATGTATGTAAACACTGTAGCAAAGCCTTCATATGCCAGAGGTCCCTTCAGTTACATAATAG AACTCATACCGGAGAAAAACCATATAAATGTGAACAGTGTGGTAACTCCTTCAGATATCACAATTCCCTT CAAAGACATAAAATAATTCACACTGGGAAAAACCTCTATGAATGTAAACAGTGTGACAGGTCCTTCATTT ATCCATATTTACTCCAAATACATGATAGAACGCACTCTGGGGGAAAACCATATGAATGTACACAGTGTGG TAAGTCCTATATTTATCCTTCTCTACTTCGAATACATGAAAGGACTCACAGTGCAGAAAAACCCTATAGC TGTAAACAATGTGGTAAGGCCTTTAAATCTCACAGTTCCCTTCGGGTCCATGAAAGAAACCATACTGGGG AAAAGCCCTATGAATGTAAACAATGTGGCAGGTCCTTTATTTATCCTTGCTTACTCCAGACCCATGAAAG AATTCACAGTGGTGTGAAACCCTACGAATGTAAACAGTGTGGGAAAGCCTTTAGAGGTCATAGTTCCTTT CGGGTCCATAAAAAACTTCACAGTGGGGAAAAGCCCTATGAATGCAAACAATGTGGTAAGTGCTTCATTT ATCCATCCTTACTTCGAATGCATGAAAGAACTCATAGTAGTGAAAAACCCTATGATTGTAAACAATGTGG TAAGGCCTTTAAATCTCGTAGTTCCCTCCGAGAACATGAAAGAAACCATATTGAGGAAAAATCCTATCAG TGTCAACAGTGTGGTAAAATCTTCAGACGTTATAGTTCATTTCAAAACCATAAACAAATCCATGCTATAC AAAAACTCAATGAATGCAAACAATGTGGTAAGATCTTCATTTATCCTTCTTTTCTTCAGAGGCATGAAAG AACTCACATTTGTGAAAGAACCCTATGTATGTAAACAATGTGATTAAATCTTTGCCATATCACAATTCTT TTAGGAGAGGTAAATCTCATTTGGGAGAATAGATATTGAATAATGTGATAAATTCTTTTTACAGTTATCA ACTGCAGAGAAAAATTTATATGTAACAGTATGAGTATAAAGTGGGATAGCCTTCTGAAGTAATCATCCTT TTCAAATGTATGGAATAACTCATAATGAAGTAGAACCATGTTTCCTACGACATTAAAAAATTGTTATGTT AGAAGGGAATTCTGTGAATATAAACAATGTGAAAACTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5323247 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5323247 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7079 same embryo
	EMAGE:7078 same embryo
	EMAGE:7082 same embryo
	EMAGE:7081 same embryo
	EurExpress:euxassay_002470 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source