EMAGE mouse gene expression spatial database

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EMAGE:7092

AI428936 expressed sequence AI428936 ( MGI:2141950)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002555. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002555_01	euxassay_002555_02	euxassay_002555_03	euxassay_002555_04

euxassay_002555_05	euxassay_002555_06	euxassay_002555_07	euxassay_002555_08	euxassay_002555_09

euxassay_002555_10	euxassay_002555_11	euxassay_002555_12	euxassay_002555_13	euxassay_002555_14

euxassay_002555_15	euxassay_002555_16	euxassay_002555_17	euxassay_002555_18	euxassay_002555_19

euxassay_002555_20	euxassay_002555_21	euxassay_002555_22	euxassay_002555_23	euxassay_002555_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7092_wholemount_strong.wlz

7092_wholemount_moderate.wlz

7092_wholemount_weak.wlz

7092_wholemount_possible.wlz

7092_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7092_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 10 11 12 13 16 17 18

pituitary gland

moderate

regional

moderate expression: see section 11 12 13 14 15 16

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 07 08 moderate expression: see section 09 17 19 20 21 22 23

dorsal root ganglion

weak

regional

weak expression: see section 10 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 14 15 16 17

kidney calyx

moderate

regional

moderate expression: see section 10 11 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5197
Entity Detected:	AI428936, expressed sequence AI428936 ( MGI:2141950)
Sequence:	sense strand is shown >T5197 CCACGCGTCCGCTCAGTAGCCTCGGACTTAGGCTGTGAGTCCTGCTGAGAAAGCATGGGGGTCTGGACTG TCTAAGAAAGCGGCTGCTGTTACTGGACATCCTCACCTGACGATCCCCAAGTGCACGGCCATGGCCCTGG TTCCACCTCTTGGCCGTGAATTCCCGCCAGAGCCTGTGAATTGTCCCCTGGCAGCTCCTAGAGAGCTGGA TGTTGTCGGAGGGACCATCTGCCCTGCACCTGAGGAAGAGACAAGCAGGCCAGAGCAGGTCCAGGCCTCC TTGGGCCTGCCTGAGCATTGCATGGGTGAACTGAAGAGCACTGAGTCTGCCACCAGCCCCTCAAGACTCC CCCTGGCCTCTTCCCATGAGCATCAAGACGGGGGCAAGCCCTGTGAGCACTCTGACTCTGGTTTGGAAGT ACTAGAAGCTGAACAGGACAGTCTACATCTTTGTCTGTTGAGGTTGAACTTCCGGCTGCAGGACCTGGAA CGAGGCCTTGGGTCTTGGACGCTGGCCCACAACAGGATTGTCCAGATGCAGGCCCTGCAGGCAGAGCTAC GAGGGGCCGCTGAGCGCGTGGATGCATTGCTTGCATTTGGTGAGGGCCTGGCAGAGAGGAGTGAGCCCAG GGCCTGGGCATCCCTGGAGCAGGTCCTGAGGGCCCTTGGAACCCACCGAGACACCATCTTCCAACGGCTC TGGCAGCTGCAGGCCCAGTTGATCAGCTATAGCCTGGTGCTTGAGAAGGCCAACCTCCTGGACCAGGACT TGGAAGTTGAGGGAGACTCAGACGGGCCAGCAGCTGGTGGAGTCTGGGGGCCCTGGGCACCCAGTACCTT CCCCACTCCTGCAGAGTTGGAGTGGGACCCAGCAGGGGATGTTGGGGGTCTTGGGCCCTCAGGGCAAAAG ATATCTCGGATACCAGGAGCTCCCTGTGAGCTGTGTGGCTACAGGGGACCCCAGAGCAGTGGACAGGGCC TTGAGGACCTGCTCTCCTTGGGGCTTGGCCACCGGAAACATTTAGCGGCTCACCATCGAAGGCGGCTCCG GAAGCCTCAGGACAGGAAGAGGCAAGTATCCCCCAGTCTGCCTGATGCAATGCTGGAAGTGGATCGCGGG GTCCCAGCTCCTGCATCCAAGCGGCCCCTGACCCTCTTCTTTCTCCTTCTCTTCCTCCTTCTGGTGGGTG CCACGCTGTTGCTGCCCTTGTCGGGGGTCTCCTGCTGTTCTCATGCCCGGCTGGCTAGGACGCCCTACCT GGTGCTCAGTTATGTCAATGGTCTCCCTCCAATCTGAGCCCACCATCCAAGACACGTGCATTAAATGGTC ACTTTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5352953 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5352953 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7090 same embryo
	EMAGE:7088 same embryo
	EMAGE:7091 same embryo
	EMAGE:7093 same embryo
	EMAGE:7089 same embryo
	EurExpress:euxassay_002555 same experiment
	MGI:4823044 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source