EMAGE mouse gene expression spatial database

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EMAGE:7101

Relb avian reticuloendotheliosis viral (v-rel) oncogene related B ( MGI:103289)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004415. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004415_01	euxassay_004415_02	euxassay_004415_03	euxassay_004415_04

euxassay_004415_05	euxassay_004415_06	euxassay_004415_07	euxassay_004415_08	euxassay_004415_09

euxassay_004415_10	euxassay_004415_11	euxassay_004415_12	euxassay_004415_13	euxassay_004415_14

euxassay_004415_15	euxassay_004415_16	euxassay_004415_17	euxassay_004415_18	euxassay_004415_19

euxassay_004415_20	euxassay_004415_21	euxassay_004415_22	euxassay_004415_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7101_wholemount_strong.wlz

7101_wholemount_moderate.wlz

7101_wholemount_weak.wlz

7101_wholemount_possible.wlz

7101_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7101_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial skeleton

moderate

regional

moderate expression: see section 07 08 09 10 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6421
Entity Detected:	Relb, avian reticuloendotheliosis viral (v-rel) oncogene related B ( MGI:103289)
Sequence:	sense strand is shown >T6421 GCCCGCCGCCCGCTCGGCCCGGCGCCCCCCGCAGCCCCGGGCGCCGTGCGTCCAGCCCGGCCTTCGGCCC TAGCCTTGCACCGCTTGCCCGGCCGGTGATCGTCCCAGCCGACCGGGCTGTCCGACTGCCCGGCTTGCCC TCGTGTGCGCTCGCCCTGAGCTGGCCTTCGGGCTGTCGGTCCCTACGGGCCGGGCCATGCCGAGTCGCCG CGCTGCCAGAGAGTCCGCGCCCGAGCTAGGGGCCTTGGGTTCCAGTGACCTCTCTTCCCTGTCACTAACG GTCTCCAGGACCACAGATGAATTGGAAATCATCGACGAATACATTAAGGAGAACGGCTTTGGCCTGGACG GGACACAGCTGAGTGAGATGCCGCGCCTGGTGCCCCGCGGGCCCGCCTCACTGAGCAGCGTCACGCTGGG CCCTGCTGCACCACCGCCTCCGGCCACGCCGTCCTGGAGCTGCACACTGGGCAGGCTGGTGTCACCCGGC CCGTGCCCACGGCCGTACCTGGTCATCACAGAGCAGCCAAAGCAGCGTGGCATGCGCTTCCGCTACGAGT GCGAGGGCCGCTCGGCCGGCAGCATCCTCGGGGAGAGCAGCACCGAAGCCAGCAAGACCCTGCCCGCCAT CGAGCTTCGAGACTGTGGCGGGCTGCGGGAGGTGGAGGTGACGGCGTGCCTGGTGTGGAAGGACTGGCCA CACCGGGTACACCCACATAGCCTCGTGGGGAAAGACTGCACGGACGGCGTCTGCAGGGTGCGGCTGCGGC CTCACGTCAGCCCCCGGCACAGCTTTAACAACCTGGGCATCCAGTGTGTTAGGAAGAAGGAAATTGAAGC TGCCATTGAGCGGAAGATCCAGCTGGGAATTGACCCCTACAATGCTGGCTCCCTGAAGAACCATCAGGAG GTCGACATGAATGTCGTCAGGATCTGCTTCCAGGCCTCCTATCGGGACCAGCAGGGACATCTGCACCGCA TGGACCCCATCCTCTCTGAGCCTGTCTACGACAAGAAGTCCACCAACACATCGGAGCTGCGGATTTGCCG AATCAACAAGGAGAGCGGGCCGTGCACAGGTGGTGAGGAGCTGTACTTGCTCTGTGACAAGGTGCAAAAA GAGGACATATCCGTGGTGTTCAGCACAGCTTCCTGGGAAGGCCGTGCCGACTTCTCTCAAGCTGATGTGC ACCGGCAGATCGCCATTGTGTTCAAAACGCCACCCTACGAGGACCTGGAGATCTCAGAGCCCGTGACTGT CAATGTGTTCTTGCAGCGGCTCACGGATGGGGTGTGCAGCGAGCCGCTGCCCTTCACGTACCTGCCTCGG GATCATGACAGCTACGGTGTGGACAAGAAGCGAAAGCGGGGACTGCCTGATGTCCTTGGAGAGTTGAGCA GCTCTGATCCACATGGAATCGAGAGCAAACGAAGGAAAAAGAAACCAGTGTTCTTGGACCACTTCCTGCC TGGCCACAGCTCAGGCCTGTTCCTCCCACCATCGGCTCTGCAGCCGGCAGACTCTGATTTCTTCCCTGCT TCCATATCCCTTCCTGGGCTGGAGCCTCCTGGTGGACCCGATCTCCTGGACGATGGCTTTGCCTATGATC CTTCTGCCCCCACGCTCTTCACTATGTTGGACCTGCTGCCCCCAGCACCACCACTTGCCAGTGCTGTGGT GGGTAGCGGGGGTGCAGGGGCCACCGTTGTGGAGTCTTCTGGCCCAGAGCCCCTATCACTGGACTCTTTT GCAGCGCCGGGCCCCGGGGATGTTGGTACTGCTAGCCTTGTGGGCAGCAACATGTTTCCCAACCAGTACC GAGAGGCAGCTTTCGGGGGTGGCCTCCTATCTCCAGGGCCTGAAGCCACGTAGCCTCTGAGGTAACAGAG GAGGCACTGGGTGAGGTATGTGGTATAGCACTCCATTCCGAAGCCAACCTTGATCAGTCTTCCAGCTTCC TCATCCTGAATCGGACATCTGCAGCGCTGGTGGGAAGATGGGGAGCACTCCGGTTCTCTTTGAGCCCATT TTACAGAATGCTGAGTCCGAAGAGGAAAAGGGGCTCCTGCAGATGGACCCCTTCTCAGGACAGATTCTCA GAGATTGTACATAGGGGAGGAGGGAGCAGGTCCCCAGCCTTCTCCCCTAATCCTGAAGAAGGCAGTGGAT TGTTCAGTTTTCCCAATAAAAATTAGTTTTTTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3584200 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3584200 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7102 same embryo
	EMAGE:7100 same embryo
	EurExpress:euxassay_004415 same experiment
	MGI:4827691 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source