EMAGE mouse gene expression spatial database

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EMAGE:7166

Lrfn4 leucine rich repeat and fibronectin type III domain containing 4 ( MGI:2385612)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002604. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002604_01	euxassay_002604_02	euxassay_002604_03	euxassay_002604_04

euxassay_002604_05	euxassay_002604_06	euxassay_002604_07	euxassay_002604_08	euxassay_002604_09

euxassay_002604_10	euxassay_002604_11	euxassay_002604_12	euxassay_002604_13	euxassay_002604_14

euxassay_002604_15	euxassay_002604_16	euxassay_002604_17	euxassay_002604_18	euxassay_002604_19

euxassay_002604_20	euxassay_002604_21	euxassay_002604_22	euxassay_002604_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7166_wholemount_strong.wlz

7166_wholemount_moderate.wlz

7166_wholemount_weak.wlz

7166_wholemount_possible.wlz

7166_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7166_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5254
Entity Detected:	Lrfn4, leucine rich repeat and fibronectin type III domain containing 4 ( MGI:2385612)
Sequence:	sense strand is shown >T5254 GCTGGCCATTCCAGAGGATGCCAGAAGGAAGTTCCCGGACCCTCCCTGCTCTTGGCCCTCCTCCACTTCC TGCCTCAGGCCTCACCTTGTTCCCAGTACCTGGACTCCCTTCAACTGCTTGGGAAATGTGACCTTTACTC TGGGGGGCCCTAGTCTGCAGCCCCAGCCATCTTTTCTCCCTGGGGTCCTCTGTGAGCCTCTTACCCAAAC CTTCCCCAGTTCAGACTCTGGAAACTGGTTCCTGGGACTGTGCCCTGGGCACAAGTGGGCACGTGCCCTA GCCCCACCTGTGCCTGGGGCTGCGGCCCTTTCCCACAGGGCACTCACCATGGCCCCGCCGCTCCTATTGC TGCTGCTGGCCAGTGGAGCCGCTGCTTGCCCGCTACCCTGTGTGTGCCAGAACCTGTCGGAGTCACTCAG CACCCTTTGTGCCCACCGAGGCCTGCTGTTTGTGCCACCCAACGTGGACAGGCGCACAGTTGAACTGCGC CTGGCCGACAACTTCATCCAGGCCCTGGGACCACCTGACTTCCGCAACATGACAGGGCTGGTGGACCTAA CATTGTCTCGAAATGCCATCACCCGCATTGGGGCTCGCTCCTTTGGGGACCTGGAGAGCCTGCGCTCCTT GCACCTGGATGGCAACAGGCTGGTGGAGCTGGGCAGCAGCAGCCTACGGGGGCCTGTCAACCTCCAGCAT CTCATTCTCAGTGGCAATCAACTGGGCCGCATCGCCCCCGGGGCCTTCGACGACTTCCTTGACAGTCTCG AGGACCTGGATGTGTCCTATAACAACCTCCGGCAGGTTCCCTGGGCTGGCATAGGCTCCATGCCTGCCCT GCATACCCTGAACCTGGACCATAACCTCATCGATGCACTACCCCCAGGTGTCTTTGCTCAGCTCAGCCAG CTCTCCCGCCTTGACCTCACCTCCAACCGCCTGGCCACCCTGGCACCTGATCCACTCTTCTCCCGAGGCC GGGATGCTGAGGCCTCACCTTCTCCCCTGGTGCTGAGTTTCAGCGGGAACCCACTGCACTGCAACTGTGA GCTGCTGTGGCTGCGGCGGCTGGCCCGGCCCGATGACCTGGAAACCTGCGCTTCTCCACCAACCCTGGCA GGCCGCTACTTCTGGGCAGTGCCTGAGGGAGAGTTCTCCTGTGAGCCTCCGCTGATTGCCCGGCACACAC AGCGCCTGTGGGTGCTAGAGGGCCAGCGGGCCACCCTACGGTGCAGGGCCCTTGGTGACCCTGTACCTAC CATGCACTGGGTTGGCCCTGATGACAGGCTGGTTGGCAACTCTTCTCGAGCCTGGGCTTTCCCCAATGGG ACCCTAGAGATTGGGGTGACGGGCGCTGGAGATGCAGGAGCCTATACCTGCATTGCCACCAACCCTGCTG GTGAGGCCACAGCCCGAGTGGAGCTCCGGGTACTGGCCTTGCCCCATGGTGGGAACACCAGTGCTGAGGG GGGCCGTCCTGGGCCTTCGGACATTGCTGCTTCTGCTAGAACTGCTGCCGAAGGCGAGGGAACTTTAGAA TCTGAACCAGCAGTGCAAGTGACGGAGGTGACTGCCACCTCTGGCCTGGTGAGCTGGGGCCTGGGGCGGC CAGCTGACCCAGTGTGGATGTTCCAAATCCAGTACAACAGCAGCGAGGATGAGACCCTCATCTACCGGAT TGTACCAGCCTCCAGCCACCACTTCCTGCTGAAGCACCTGGTTCCCGGTGCTGACTACGACCTCTGCCTG CTGGCCCTGTCACCTGCTGCTGGGCCCTCCGACCTCACGGCCACTAGACTGCTGGGCTGTGCCCACTTCT CTACGCTACCAGCCACCCCCCTGTGCCATGCCCTACAGGCCCATGTGCTGGGCGGGACCCTGACTGTGGC AGTGGGGGGCGTCCTAGTGGCTGCCTTACTGGTCTTCACTGTGGCCTTGCTGGTTCGGGGCCGGGGAGCT GGGAATGGCCGCCTCCCACTCAAACTCAGCCATGTCCAATCCCAGACCAATGGTGGCACCAGCCCCATGC CCAAGAGCCACCCACCACGGAGCCCTCCACCCCGTCCTCAGCGCAGCTGCTCCCTGGACCTAGGAGACAC CGGAGGATGCTACGGGTATGCCAGGCGCCTGGGAGGAGCCTGGGCCCGGCGGAGCCACTCTGTACATGGG GGGCTGCTGGGAGCTGGGTGTCGGGGGGTGGGGGGCAGTGCGGAGCGGCTGGAGGAGAGTGTGGTGTGAC AGAAAGGGCAGCCTCTTATGTGCCCTGAGGAAGGAGCAGTGCCACTGTTGGGCCCAGGTGGCAGCACCCA GCCTGGGTGGGCAGCACGGCCCTGGGTCCCACTCTGCTTTTTATCCTCAGTACCTCAGGCCCCCATGTAC TTGGTGGGACAGCAAACCCTTTCCTTGGTTTTGGCCTCCAGACTAAAGGGACAGGTCCCACCAACAGGTG CTCAGAGCCACCAAGGCAGGGGCTGCAGCCACCAAGAGGGAGTCTTGTTTCTATTTATAATAAAATTGTT GGGGATAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5358628 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5358628 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7164 same embryo
	EMAGE:7163 same embryo
	EMAGE:7165 same embryo
	EurExpress:euxassay_002604 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source