EMAGE mouse gene expression spatial database

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EMAGE:7168

Loxl1 lysyl oxidase-like 1 ( MGI:106096)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002603. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002603_01	euxassay_002603_02	euxassay_002603_03	euxassay_002603_04

euxassay_002603_05	euxassay_002603_06	euxassay_002603_07	euxassay_002603_08	euxassay_002603_09

euxassay_002603_10	euxassay_002603_11	euxassay_002603_12	euxassay_002603_13	euxassay_002603_14

euxassay_002603_15	euxassay_002603_16	euxassay_002603_17	euxassay_002603_18	euxassay_002603_19

euxassay_002603_20	euxassay_002603_21	euxassay_002603_22	euxassay_002603_23	euxassay_002603_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7168_wholemount_strong.wlz

7168_wholemount_moderate.wlz

7168_wholemount_weak.wlz

7168_wholemount_possible.wlz

7168_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7168_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5022
Entity Detected:	Loxl1, lysyl oxidase-like 1 ( MGI:106096)
Sequence:	sense strand is shown >T5022 CCACGCGTCCGCGCCAGCCTAGCGAGCAGCCAGCCAGTGTGGGGCTGGCCATGTAAGGGCCCCAGGAGGT CCTGCCCGTCCCGTGCCCTACGGAAAGCCTCGTGCAGCCCTGGGCACCGCCCCTGCCCTGCCCTGACCCC TTGGCCTCGAAATGCTGTCATCGGAGGAGCCGTCCCGCTCGGGACAAGGCCAGGATGGACAAAGCTAGAG CTTGGGCAGGCGAAGAGCCATCCTGTCCTCGAGGCCGTGGGAAGAGAAGCACGCACCGAGGCCCACTCTC TGCCCACCCGGCCTCTGCAGAAGGGCCACTATGGCTCTGGCCGGAGCCGGCAGCCAGCTGAGGACCCTGG TGTGGAGTGCCTGCCTGTGCGTCCTGGTGCACGGGCAGCAAGCGCAGCCAGGACAGGGCTCGGACCCAGG ACGCTGGCGGCAGCTGATCCAGTGGGAGAACAACGGGCAGGTGTACAGCTTGCTCAACTCGGGCTCTGAG TACGTGCCCGCGGGGCCCCAACGAGGGGAGACTAGCTCCAGGGTTCTACTGGCAGGCGCGCCCCAAACGT CGCAGCGGCGCAGTCAGGGGGGACCCCGGCGTCGGCAGGCGCCGTCCCTGCCCCTGCCCGGACGCGTGGG TTCGGACACTGTGCGCGGCCAAACGCGGCACCCGTTCGGCTTCGGCCAGGTGCCCGACAACTGGAGAGAG GTGGCCGTCGGGGACAGTACCGGCATGGCTCGGGCCCGCACCTCCGTCTCCCAGCAACGGCACGGGGGCT CCGCCTCCTCCTCGGTTTCGGCCTCGGCCTTCGCCACCACCTATCGCCAGCCATCCTCCTACCCGCAGCA GTTCCCCTATCCGCAGGCGCCCTTCGTAAACCAGTATGAGAACTACGATCCCGCGTCACGGACCTACGAA CAGGGCTACGTGTACTACCGTGGTGCGGGCGGCGGCATGGGCGCGGGGGCAGCGGCTGTAGCCTCGGCTG GGGTCATCTATCCCTTCCAGCCCCGGGCGCGTTACGAGGACTACGGAGGTGGAGGAGGCGAGGAGCAGCC CGAGTACCCGGCCCAGGGCTTTTACCCAGCCCCAGAGAGGCCCTATGTGCCCCAGCCCCAACCCCAGCCC CAGCCCCAGCCGCAGCCTCAGCCCCAACCTTCCGACGGCCTTGACCGTCGTTACTCGCACAGCCTGTACA ATGAAGGCACCCCGGGATTCGAGCAGGCCTACCCTGATCCCAGCACCGACGTGTCGCAGGCCCCCGCGGG GGCCGGTGGCACCTACGGTGGCGCTGGCGATCCTCGCCTCGGCTGGTACCCGCCCTATGCAGCCAATGTG CCACCGGAGGCCTACGTCCCTCCGCGTGCGGTGGAGCCACAGCCCCCATTCCGCGTGCTGGAGCCACCTT ACTTGCCCGTGCGAAGCTCTGATGCGCCCTCCCAGGGTGGGGAGCGGAACGGCGCCCAGCAGGGCCGTCT CAGCGTGGGTAGTGTGTACCGACCCAACCAGAATGGTCGCGGCCTCCCTGACTTAGTCCCGGACCCTAAC TACGTGCAGGCATCCACATACGTGCAGAGAGCCCATCTGTACTCCTTGCGATGCGCAGCAGAGGAGAAGT GCCTGGCCAGCACAGCCTATGCCCCTGAGGCTACCGACTATGACCTCCGAGTGCTATTGCGCTTCCCCCA GCGAGTGAAAAACCAAGGCACAGCGGACTTTCTCCCCAACCGGCCACGCCACACCTGGGAGTGGCATAGC TGTCACCAACATTACCACAGCATGGACGAGTTCAGCCACTACGACCTGCTTGATGCATCCACGGGCAAGA AGGTAGCCGAGGGACACAAGGCCAGCTTCTGCCTGGAGGACAGCACCTGTGACTTCGGCAACCTCAAGCG CTATGCCTGCACCTCTCACACGCAGGGCCTGAGTCCAGGCTGCTATGACACATACAATGCGGACATTGAC TGCCAGTGGATCGACATAACTGATGTGCAGCCTGGGAACTACATCCTCAAGGTGCACGTGAACCCCAAGT ACATTGTTCTGGAGTCTGACTTCACCAACAACGTGGTGAGATGCAACATCCACTACACAGGTCGCTACGT TTCTACAACAAACTGCAAAATCGTCCAGTCCTGATCTGAGTGGACAGAGCCCCCCTTGTCTCTCCTTGAA AAGCAGGACCTGCTTCCAGGCAGCCTCCCCCATGAGGCTCAGCCGCCTAGCCCAAGGCAAAGGCAGCTGG CCGCTGGGGCATCCCTCCCTGCCGGCCTCAGGGAGTGAACATGGACCCAAACCACAGGGATTCTGGATGC CAGACCCTGTCTTGTACCTACTTCTCCTCTACAACACCATGGTGTCTGTTGTTGGTTCTAATTTTTATAT TTTGGCCATCCCACACAGCAAGATTGCCCGGGTGCTGAATAAAACAAGGGTTTTCTAGTCTGGAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5251968 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5251968 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7170 same embryo
	EMAGE:7172 same embryo
	EMAGE:7169 same embryo
	EMAGE:7167 same embryo
	EMAGE:7171 same embryo
	EurExpress:euxassay_002603 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source