Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7178

Crybb1 crystallin, beta B1 ( MGI:104992)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7178 EMAGE:7178 EMAGE:7178 EMAGE:7178 EMAGE:7178
"Pseudo-wholemount" of euxassay_002621. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002621_01 euxassay_002621_02 euxassay_002621_03 euxassay_002621_04
EMAGE:7178 EMAGE:7178 EMAGE:7178 EMAGE:7178 EMAGE:7178
euxassay_002621_05 euxassay_002621_06 euxassay_002621_07 euxassay_002621_08 euxassay_002621_09
EMAGE:7178 EMAGE:7178 EMAGE:7178 EMAGE:7178 EMAGE:7178
euxassay_002621_10 euxassay_002621_11 euxassay_002621_12 euxassay_002621_13 euxassay_002621_14
EMAGE:7178 EMAGE:7178 EMAGE:7178 EMAGE:7178 EMAGE:7178
euxassay_002621_15 euxassay_002621_16 euxassay_002621_17 euxassay_002621_18 euxassay_002621_19
EMAGE:7178 EMAGE:7178 EMAGE:7178
euxassay_002621_20 euxassay_002621_21 euxassay_002621_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7178Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7178_wholemount_strong.wlz
7178_wholemount_moderate.wlz
7178_wholemount_weak.wlz
7178_wholemount_possible.wlz
7178_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7178_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5255
Entity Detected:Crybb1, crystallin, beta B1 ( MGI:104992)
Sequence:sense strand is shown

>T5255
CACCAATACAGCACCAGGAACCATGTCCCAGGCTGCCAAGGCTTCAGCCACCACAGCGGTGAACCCAGGG
CCTGATGGCAAGGGAAAAGGGGCCCCATCCACAGGGCCAGCCCCTGCCCCTGGCCCCACTCCTGTCCCAG
CTTCTGTGCCCAGGCCCGCTGCCAAAGTAGGGGACCTGCCTCCTGGGAGCTACAGGCTGATCGTCTTCGA
GCAGGAAAACTTTCAGGGCCGCCGGGTGGAATTCTCGGGGGAGTGCCTGAATCTGGGGGACCGTGGCTTC
GACAGAGTGCGCAGCCTGATCGTCGTCTCGGGACCCTGGGTGGCCTTTGAGCAATCTGCCTTCCGTGGAG
AGATGTTTGTCCTGGAGAAGGGCGAGTACCCACGCTGGGACACCTGGACCAGCAGTTACCGCAGTGACCG
GCTCATGTCCTTCCGGCCCATCAGGATGGATTCCCAGGAACATAAGATCTGCCTGTTCGAAGGGGCCAAC
TTCAAGGGCAACACCATGGAGATCCAAGAGGATGATGTGCCCAGCCTCTGGGTTTATGGCTTCTGTGACC
GTGTGGGCAGCATAACCGTCTCTGGTGGCACATGGGTCGGCTACCAGTACCCAGGTTACCGTGGCTACCA
GTATCTGCTGGAGCCTGGCGACTTCCGGCACTGGAACGAGTGGGGCGCCTTCCAGCCGCAGATGCAGGCT
GTGCGGCGCCTGCGGGACCGCCAGTGGCACCAAGAGGGCTGCTTCCCGGTGCTGACAGCCGAGCCCCCCC
AAGTGAGACAAGCTCCCGGCCTCCCTTCTACAGCCCGCCCTGCCTCACGTCCCATCTTCCCCTACTAATA
AAGTTCTTATAGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5358714 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5358714 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7175 same embryo
 EMAGE:7173 same embryo
 EMAGE:7174 same embryo
 EMAGE:7177 same embryo
 EMAGE:7176 same embryo
 EurExpress:euxassay_002621 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS