EMAGE mouse gene expression spatial database

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EMAGE:7207

Leprot leptin receptor overlapping transcript ( MGI:2687005)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004408. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004408_01	euxassay_004408_02	euxassay_004408_03	euxassay_004408_04

euxassay_004408_05	euxassay_004408_06	euxassay_004408_07	euxassay_004408_08	euxassay_004408_09

euxassay_004408_10	euxassay_004408_11	euxassay_004408_12	euxassay_004408_13	euxassay_004408_14

euxassay_004408_15	euxassay_004408_16	euxassay_004408_17	euxassay_004408_18	euxassay_004408_19

euxassay_004408_20	euxassay_004408_21	euxassay_004408_22	euxassay_004408_23	euxassay_004408_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7207_wholemount_strong.wlz

7207_wholemount_moderate.wlz

7207_wholemount_weak.wlz

7207_wholemount_possible.wlz

7207_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7207_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6416
Entity Detected:	Leprot, leptin receptor overlapping transcript ( MGI:2687005)
Sequence:	sense strand is shown >T6416 CCCACGCGTCCGGCAGCGACAGCCCCAGCTCCAGAGACATGGCGGGCGTTAAAGCTCTCGTGGCGCTCTC CTTCAGTGGGGCAATTGGGCTGACTTTTCTTATGCTGGGATGTGCGTTGGAGGACTATGGCGTTTACTGG CCCCTGTTCGTCTTGATTTTCTACGTCATCTCCCCCATCCCCTACTTCATTGCCAAAAGGGTCACATATG ACTCCGACGCGACCAGCAGTGCCTGTCGGGAACTGGCGTATTTCTTCACCACTGGGATTGTTGTTTCTGC CTTTGGACTTCCTGTTGTTCTTGCTCGTGTGGATGTGATCAAGTGGGGAGCCTGTGGTCTTGTGCTGGCC GGCAACGCTGTCATTTTCCTCACAATTCAAGGCTTCTTCCTCGTCTTTGGAAGAGGAGATGATTTCAGTT GGGAACAGTGGTAGCCCCTCCTCCCCATGGAAAGCATTGAGTCTTAGAATTTGTACTGTACGTATATGTG TGCACATGTGTTTCAGATTTGAATATGCTGAGTTTTTTTATACCTTTATATCATGATCATCTTAGGGAAG ATGTTGTAAACAAAAGATGAGTTTTACTCCTGGCACACAGGTCACTCCATTGACTCAAAGTGAATTATTT TTATCTTCTAGCTTCCAGAAAGCCTGTTACTATGGCCTTGTAGACAGCTGCTCACTTATCCAGGGGGTCT AAACCTTTTGCCTTGGGTTAGGCTTGGGAGACACTGGGGCAGATCTCCTACAGCCCAAACATAGGTGGCG CTCTCCCTGTACACTTAGACTCATCTCATCCACACAGAGCATCAGCATCTGCTAGGAGCCCATTGGGGAT GCAGGGTCAGACTGGGTAACTTTGATGCGATTGCACACTTGGCGCACGCACATTGACGGGTGAGGAGGTT TGTTGTAGAGGAATAAGCATCATAGCTCCCCTGCCCTCTCTGGGTGTTGCATGCTCATTTGCAGGAAAAA AAAAAAAATCACCTGCCCCCTGCTGTGAATGAGTTCAGACTTCAGGTGTTTCTAACATGTTCAATACTTG TGTTGTTCCTCAAATTTGGGGGACCTGTTCTTAGGCAGGAACAGATAAGTCATCCAAAAGGCAGTGGCTG ATTGCTCAGAATAAGATACAAAATAACCAAAAAACCCCTCTGGGGTGCAAGGAGGGAAACTTGGCTGGAA TGGTCCCTACTTTAAGTGGAGGGCAGGATAGACAATTCTTGGAAATGTCCCTCTACGGTGCCTCCCATCC CCCATGCAGCATTTGGTTTGCTCTTGGTGGTGGGTCTCCTGAAGGGTATGAGGCAAGAGTGAGCAGAGGG GAGGGAGTCACTGAAGTTACACGCTGGTGCACTTTTCATCCTTGATATTGAACTTGATGTCACCTCCACA GGACACACAGGGATCCACATCATTTTGGTTCTTATAAACAGTTTTTCTAGCTCATCTTTTTTATCTGGAG CCTACTGGGAGAAGGAAGTGAGAACTACAAGCACCCAGTCAGGATGCCATCAGCGGTGTTTAAGCATTGA TGCTGCTCGCTAATGAGTGTCCAGAGCACTCTCCCTTGAGGCTTGCGGTCCAAGGAGTCAGCATCCAGGC CAGGGCTGGTGTGACGCTGGGACCAGCAGGTGAATGGACAGAGGACAGTGTGCAATGTTATATTTTGGCC TTGTGCATGACATTATGTTTTGAATATTTTGTGTACAAATAGAATTGTTAGCGTTGTCATTTCTAGTATT TACATTAGAAAAATTATTTAGATACTTGATCATTTAACTGGCATTTTTAATCATGACACTTGCTATTGTA ATAAATTTGACTTTTAACACTGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3583760 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3583760 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7204 same embryo
	EMAGE:7203 same embryo
	EMAGE:7202 same embryo
	EMAGE:7206 same embryo
	EMAGE:7205 same embryo
	EurExpress:euxassay_004408 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source