EMAGE mouse gene expression spatial database

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EMAGE:7217

Eefsec eukaryotic elongation factor, selenocysteine-tRNA-specific ( MGI:2137092)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002716. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002716_01	euxassay_002716_02	euxassay_002716_03	euxassay_002716_04

euxassay_002716_05	euxassay_002716_06	euxassay_002716_07	euxassay_002716_08	euxassay_002716_09

euxassay_002716_10	euxassay_002716_11	euxassay_002716_12	euxassay_002716_13	euxassay_002716_14

euxassay_002716_15	euxassay_002716_16	euxassay_002716_17	euxassay_002716_18	euxassay_002716_19

euxassay_002716_20	euxassay_002716_21	euxassay_002716_22	euxassay_002716_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7217_wholemount_strong.wlz

7217_wholemount_moderate.wlz

7217_wholemount_weak.wlz

7217_wholemount_possible.wlz

7217_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7217_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5906
Entity Detected:	Eefsec, eukaryotic elongation factor, selenocysteine-tRNA-specific ( MGI:2137092)
Sequence:	sense strand is shown >T5906 GCGCTCAGGCGACGGCGTGAGAGGCAGCGGCGGGATGGCCGGCCGGCGGGTAAATGTGAATGTGGGCGTG CTGGGTCACATCGACAGTGGCAAAACAGCGCTGGCGCGCGCGCTGAGCACCACGGCCTCCACTGCTGCCT TCGACAAGCAGCCGCAGAGCCGCGAGCGCGGCATAACGCTCGACCTGGGCTTCTCCTGTTTTGTCGTGCC GCTGCCGGGGGCAGAACCCGGTTCTAGCGACACGCTGCTGCAGGTCACACTGGTCGACTGCCCCGGGCAC GCCTCCCTCATCCGGACCATCATTGGCGGTGCCCAGATCATTGATTTGATGATGCTCGTGATTGATGTGA CCAAGGGAATGCAGACACAGTCCGCAGAGTGCCTTGTGATTGGCCAGATCGCCTGCCAGAAGCTGGTTGT GGTGCTGAACAAAATAGACCTGTTAGCAGAAGGGAAGAGACAGGCAGCGATTGACAAAATGACCAAGAAA ATGCAGAAGACTCTTGAGAACACCAAATTCCGGGGTGCACCGATTATTCCCGTGGCAGCCAAACCTGGGG GACCAGAAGCCCCTGAAACTGAAGCTCCACAGGGCATCTCAGAACTCATTGAGCTCCTGAAGTCCCAGAT TTCCATCCCAACGAGAGACCCCTCTGGACCATTCCTTATGTCTGTGGACCACTGCTTCTCCATCAAGGGT CAAGGCACCGTGATGACTGGGACTATCCTCTCAGGCACTATCAGCCTTGGGGACAGTGTGGAGATCCCTG CCCTCAAGGTGGTGAAGAAAGTGAAGTCCATGCAGATGTTCCATACACCCGTCACCTCTGCCATGCAGGG GGACCGACTGGGCATCTGTGTCACCCAGTTTGACCCCAAGCTGTTGGAGCGTGGGCTGGTATGTGCACCC GAGTCTCTGCACACTGTTCACGCAGCCCTCATCTCTGTGGAGAAGATCCCTTACTTCCGAGGACCTCTGC AGACCAAGGCCAAGTTCCATATCACCGTTGGCCATGAGACAGTCATGGGTCGCACGCTGTTCTTCAGCCC TGCTCCTGACAGCTTTGACTTGGAGCCCGTGCTAGATTCCTTCGACCTTTCCCGAGAGTACCTCTTCCAA GAGCAGTACCTGTGCAAGGATTCCATGCCCACAGCCACAGAAGGTGATGACGAGGCGGACCCCAAAGCCG GCCACGCCCCAGGGGGCCACTGCCCCCGGCAGCAGTGGGCCCTGGTAGAGTTTGAGAAGCCAGTCACCTG CCCCCGACTCTGCTTGGTGATTGGCTCCAGACTAGATGCCGACATTCACACCAACACATGCCGGCTGGCC TTCCATGGTGTCTTGCTGCAAGGGCTGGAGGACAAGAACTACATTGAGAGCTTCCTGCCAGCACTGAGGG TTTACAAGCTGAAGCACAAGCATGGCCTTGTGGAGCGGGTAATGGATGACTACAGCGTGATTGGACGTTC CCTGTTCAAAAAGGAGACCAACATCCAGCTCTTTGTGGGGCTTAAGGTGCAGCTGTCCACAGGGGAACAG GGTATCATCGACAGTGCCTTCGGCCAAAGTGGCAAGTTCAAGATCCACATCCCTGGTGGCCTCAGCCCTG AGTCCAAGAAGATCCTGACACCTACACTCAAGAAGCGGAGCCGAGCCGGCCGTGGAGAGACCACCAAGCC AGAGGAGGGCACTGAACGGCCAGAGCCCATCCAGCCTGTGACACTCAACCTGTCTTTCAAGCGCTATGTC TTTGACACCCAGAAGCGCATGGTACAGACTCCCTGAATGAACCATCCTGCCCAGCTGCAAGCCAGACCAC AGTGCCAGATGCCCCCAGACATGCCTCAACCTCCCCAGTCTGAGGCAGTCAACCCCATCCCACTGCCATT GCACTGTTATTGAGCCCCATTGAGAAAACCTGGGGAAAATGAGGAACAGTGCCAGGAGGGCCTGCCACTC CATCCCTGAACCCACTCAGGATAGCAACGAGGCCTACTAGAAAATGGCCTTAGCAGAGCCAGCAACCTGG GTCTTCCCCCGATAAGAGTGTGCAGCCACTCAGCCTTGCCCCAACTAGCTGGCACCCTCCAACGGAAAAT AAACATCCCATATGTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3158026 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3158026 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7215 same embryo
	EMAGE:7214 same embryo
	EMAGE:7218 same embryo
	EMAGE:7219 same embryo
	EMAGE:7216 same embryo
	EurExpress:euxassay_002716 same experiment
	EMAGE:21612 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source