EMAGE mouse gene expression spatial database

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EMAGE:7241

Tmem176b transmembrane protein 176B ( MGI:1916348)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004381. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004381_01	euxassay_004381_02	euxassay_004381_03	euxassay_004381_04

euxassay_004381_05	euxassay_004381_06	euxassay_004381_07	euxassay_004381_08	euxassay_004381_09

euxassay_004381_10	euxassay_004381_11	euxassay_004381_12	euxassay_004381_14	euxassay_004381_15

euxassay_004381_16	euxassay_004381_17	euxassay_004381_18	euxassay_004381_19	euxassay_004381_20

euxassay_004381_21	euxassay_004381_22	euxassay_004381_23	euxassay_004381_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7241_wholemount_strong.wlz

7241_wholemount_moderate.wlz

7241_wholemount_weak.wlz

7241_wholemount_possible.wlz

7241_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7241_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 06 07 moderate expression: see section 02 03 08 09 10 11 18 19 20 21 22 23

ventral grey horn

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 16 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 15 16 18 19 20

neural retina

weak

regional

weak expression: see section 01 02 03 04 23 24

kidney calyx

moderate

regional

moderate expression: see section 21 weak expression: see section 08 09 10 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6388
Entity Detected:	Tmem176b, transmembrane protein 176B ( MGI:1916348)
Sequence:	sense strand is shown >T6388 CCACGCGTCCGCGGACGCGTGGGTCACGGCCCCTTGGTAGCCCCCTTTTGAAGCATAGGGTTCCTTCTTC TCTGGAAGAGTCTCCAAGTCTACTCCTCAAGCTCCATCTTGGCTCAGGATGCTTCTCCTTCCTTCTATCT CTGCTTTCCTGTAGGACTCTGGGGAGGGAGGGATGTGTTGGACAGAGGATGTGACTCTGGAAACAGGGCT GAGGCTGGGGAGGTCAGAGTCTGGCTTTACCTTCCATAGTTATCAGTGCCTCACGCCTGCGTTCTAAGAG CAGGGCCTTTGAGACCACGCAGAGGTGGGACAGCTGTGGGGACACGGGGTGTTCTTTTTCTTTGTTTCTG AACACTGGGAGGAGGGATATCAGAGAGCCACTATCTGGAGCGGTGCCAGAAGTGGTGAGAGGCTTCTTGG TGCCTAGGGCTTTAGTTCCTGCTCTGCAGAGGAAGCAGAGGGATTCTGGGTGGCCAGGGCAGGGATAGCA CTGGGGGTCTAGAGGGGGCCAGCACCCAGACAACTGAGACTTTATTCCTGGAACCTCAAGGGAAGGTGTG AACCTGAGGCCTTCATTTGTGTTTCTCTTCGTCCCTCCTGAAAAGTTGTCAGACTCCTGTGCTTTCGGCT CTGAGGTGCACGGCAGGAAAGATGGTCCAGAGCACAGTGACTGTGAATGGAGTCAAAGTGGCCTCCACAC ATCCCCAGTCCGCTCACATCAGCATCCACATCCACCAAAAATCTGCTTTGGAGCAGCTGCTGGGAGCTGT GGGCTCCCTGAAGAAGTTTCTCTCCTGGCCTCAGGCCAGAATCCACTATGGGCAGCTGTCTCTAGGGGTG ACCCAGATATTGCTGGGGCTTGTGAGCTGTGCTCTTGGAGTATGTCTCTACTTCGGGCCTTGGACTGAGC TGTGCGCCTTTGGCTGTGCTTTCTGGTCGGGGTCTGTGGCAATTCTAGCTGGAGTTGGTACTATTGTCCA TGAGAAGAGGCAGGGAAAACTGTCCGGCCAGGTATCATGTCTCCTCCTCCTGGCTTGCATTGCCACAGCT GCAGCTGCTACCGTTCTGGGTGTGAACAGCTTAATCAGGCAAACCAGTGTTCCCTACTACGTCGAGATCT TTTCCACATGCAACCCCTTGCAATCAAGCATGGACCCTGGGTATGGGACAGTGCGATACAGTGATGATTC AGACTGGAAGACAGAGAGGTGCAGAGAGTACCTGAACATGATGATGAACTTGTTCCTAGCGTTCTGCATC ATGCTCACAGTGGTCTGTATCCTGGAGATTGTTGTGTCTGTGGCTTCCCTGGGACTAAGTCTCCGAAGTA TGTATGGCCGGAGCTCCCAGGCCCTGAATGAAGAAGAGTCAGAGAGGAAGCTTCTTGATGGGCATCCAGC ACCTGCGTCCCCGGCCAAAGAGAAGATCCCTGCTATCCTGTGATCCGCCCACACCCACGTGTCCCTGTCC ATAGTAATCCAGGGAGGACGCGACTGTTCCCAGGGCCTCTTCTGGCCCCGCAGCACACACCTACTTCTGA TGTAGCCACTATTCAGCCCTCTTTCTCTCCCTCTCTCCCCACTGTCCTCACGCCAATGTCCTTTCTTCCA CGAACAGATATTATGTCATTTTATCCAATGCCGATAAAGGAGAAACAACTTGAAAATAAAAAAAAAAAAA AAAG
Notes:	The probe template was PCR amplified from IMAGE:3582123 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3582123 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7238 same embryo
	EMAGE:7237 same embryo
	EMAGE:7239 same embryo
	EMAGE:7240 same embryo
	EurExpress:euxassay_004381 same experiment
	MGI:4828785 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source