EMAGE mouse gene expression spatial database

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EMAGE:7324

6430548M08Rik RIKEN cDNA 6430548M08 gene ( MGI:2443793)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004350. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004350_01	euxassay_004350_02	euxassay_004350_03	euxassay_004350_04

euxassay_004350_05	euxassay_004350_06	euxassay_004350_07	euxassay_004350_08	euxassay_004350_09

euxassay_004350_10	euxassay_004350_11	euxassay_004350_12	euxassay_004350_13	euxassay_004350_14

euxassay_004350_15	euxassay_004350_16	euxassay_004350_17	euxassay_004350_18	euxassay_004350_19

euxassay_004350_20	euxassay_004350_21	euxassay_004350_22	euxassay_004350_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7324_wholemount_strong.wlz

7324_wholemount_moderate.wlz

7324_wholemount_weak.wlz

7324_wholemount_possible.wlz

7324_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7324_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5266
Entity Detected:	6430548M08Rik, RIKEN cDNA 6430548M08 gene ( MGI:2443793)
Sequence:	sense strand is shown >T5266 GCTGGAGCAGGTAGAGGTGTGGTGTGCTGCTTAGCAGGGAGGGATCCTGATGCTGGCGTCCCTGGACTCC TTCAGTTAGTGTGCCCGTGTCATGCTTCTGCATAGCAGCAAAGGGGACATGGCTGTTGGCATCCCGGACT CTGAGACGCCACAGGGCCTCTGAGCTGCAAGAGACTTCGGCCGTGGTGGCCAGGCGCTCCCCACACATTG TATACGCTTGCAGCTTCAGGAAGCTGCTCCTCAGGCTGTGCCCATCCTGTTTGGCAGCCCAGCTACCATG GAGGCCCCAGAAGTCCCTGTGGGCTCCCTGATTGACTTTGAGACTGAACCACCTACCTCCCCTCCTTTGG AAGCAGCACCGTCAACGCTCCAGGACCCAGATGGCTCCCTGGGTGATGGTGCATCTGAGAGTGAGACCAC CGAGTCCGCAGACAGTGAGAACGACATGGGCGAGTCACCTTCACACCCATCCTGGGACCAGGACCGACGC TCTTCCTCCAATGAGTCCTTCTCCTCCAATCAGAGTGCTGATTCCGCCCCAGATGAGGAGACCCTGGCAC TTCGAGAGTTTATGCGCAGCTACGTAGAGAAGATCTTCTCTGGAGGGGAGGACTTGGACCAGGAAGAGAA AGCCAAGTTTGGGGAGTACTGCAGCGGAGAAGACGGGAAAGGCCGGGAATGGTTCGCACGCTTTGTGAGC GCACAGCGCTGCAAGTCCAAGTGTGTGTCGGAGCCAACCTTCTACCGCCTGGTGCAGTCCTTCGCGGTAG TGCTGTTTGAATGCCACCAGATGGACGACTTCGGGCCTGCCAAGAACCTCATGACCATGTGCTTCACTTA CTACCATCTGGGCAAGCCCCAGCTGCCCCCGACAGAGCCCAGGGAGAAGCCAGCAGGCAGTATTGACTCC TACCTGAAGTCAGCCAACAGCTGGCTGGCAGAGAAGAAGGACATTGCCGAGCGGCTGCTGAAGAACACAG AGAACATGAAAGGCTTTTTCGGGGGGCTGGAGACCAAGCTGAAAGGACCGCTGGTCAGGAAAAATGAGGA AGATGAGAACAAACCTAAGGACAGACAGACGAAGACTGTGACCATGATCAGCCCTGAGGATGAGCAGAAG GGGGAGAAGGTTTACCTGTACACGCACCTGAGGCAGCAGCCCATCTGGCACACGCTGAGGTTCTGGAATG CTGCCTTTTTTGATGCTGTCCATTGTGAGAGGCGGAAGCGATCCCCCACCACCAGAGGGGATGCTGGAGA GCAGGAGGAGAAGAGGGAGAAATGGTGCCACATGACCCAGGAAGAACGGGATGACAGCCTGCGCTTCAAC GAGAACATCACCTTCGGGCAGCTGGGCACGTTCACCCACAACATGCTGGCCTTTGGCTTGAACAAGAAGC TATGCAGCGACTTCCTGAAGAAGCAGGCTGTGATTGGCAATCTAGATGAAGAGCAATACAAGCTGCTAAG TGACCACATCGAGCAAATGGCCACAGAGTAGGAGATCAGCACGTGCTCGTCTCCTAGGATCTGTCACCCC AGTGATGACCCTGCATGACACCAGCAGCACCCAGAGCCGCTCCTTGCTGCCCTAGAACTAGCAGTTAAAA GACTCCAGAAGCCTGTCTCCCCTGCCTGTGTCTGCTCTCCCTCTATGTCTATGGTGTCCCCTGGGTTACA CAGCTAAACGTGAGGGACCATCTACCCCCCACCTATCCTCGAGCACCAGGCTTGTGAGATGAGACAGAGG GTGGAGGAAGCCATGAGGGATGGTCCCCACGGATCCTGCCGAGAGCCACGGACATGTGGTTACAGAGGCG CAGGAGGGGATCCACACAGCTGCAACGTGAGGACAGAATCAGTGCTGTGAATGACAGCCTGCCACTCCCT GCCACAGGACTCCCTGTGAGCTGTACAGAGGACCATGGAGCCTTCCTGAAAGGTCTCGGCCATCTGTTCT TCTCCACCTCCTCATGGGATAGGAACTGAAAGAGAACTCAAGGGTTGTCACTTTCCTAGAGGGTGTGAGC GTTCTGCCAGCCACAACCATGTCTCAGACTCAAGAGCTCCGTCAACAGAGCCTCGGAACTGGCCACTCAA TACAGTCTCTCCCTCAATAGCACAGAAGGTCCTTGAGCTCCCAGGCCGCAGCAACACATGCAGTGGGACG CAGGAAGCGTGGGCGAGCATTCTCCTCGGCAGCTCTGGGGGGGACAGAGCTAAGAGCACACAGAATGGAT CTGACTGAGGGAGGAGACCACGGGAAAGCAAGGTTGCCTGGTAGCTCCAGCCCACAGTCCTCCCCTTCTG AAAGACCCACAGCGATCTGCCTCCTCAGCATCCAGCGTGCTGGCTCCAGAGCTGCTGTTCCCATCCACGC CAGTGGCCTCGGTCCCTTCTGTGCCAGGCATTCTGGTCACTGACCTTGAACTAGGCCTGCAGGCTCTCCT GTCACCCCGCATCACCTCACCCTTCTCTGTCCCTCAGCCTTCAGTCACATTTAGTCATGGTCTGCCTGTG AGCGCAGTGAGTTCTCTCCTGACAGCTGTGAGCTCAGCTGGTGTGGGTGAGCCTTTCCTGCAGGAAGAGC CTCCCAAGGGATGGCCAAAGAAGAGCAGGATAGGCAAAGCTGTGGAATGTACATTTCCTGGATGTCAGGG GTGACCAAAACAGTACATCAGTTCCTGAGATATTCAAGCAAATGCCCCCGATAGCATAGTGTGTGCCAAC CGGTACACCTGACTCAGCATTAGGATCCTATAACCATGCCTGTCCCTGCTCTGTGTTTTAGGGAAGTGAC ATAAAGTGAGGTCGCCCTTGCTAAGTCCTGCTGTCCTCTCTAGAGCCTTCCTCAGAGCTGTGCAAGCATG GGAGCCTCTCCTTGCTCCTCCCAGTCCTGCACTGGCATGAGTTCGGGTGTAGGAGGACATGACTCCAAGG TGCTGGGAGATGCACCAGTCTTGCGCAGCAGCACCTGTGGTCTGGCCTCTGCCTTGCCCTCATGCAGCCA TGAGCTGCCTTAAGGGGAAGAGTGCCAATGGTCTTCGTGGACTTCAGAAGTGACTTTGAGACTGTTCCTG CTGACCTTTTGAGATAGTCACTTAAGGAAGGATGGCTCTCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5359842 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5359842 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7320 same embryo
	EMAGE:7322 same embryo
	EMAGE:7321 same embryo
	EMAGE:7325 same embryo
	EMAGE:7323 same embryo
	EurExpress:euxassay_004350 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source