EMAGE mouse gene expression spatial database

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EMAGE:7332

Reep5 receptor accessory protein 5 ( MGI:1270152)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004460. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004460_01	euxassay_004460_02	euxassay_004460_03	euxassay_004460_04

euxassay_004460_05	euxassay_004460_06	euxassay_004460_07	euxassay_004460_08	euxassay_004460_09

euxassay_004460_10	euxassay_004460_11	euxassay_004460_12	euxassay_004460_13	euxassay_004460_14

euxassay_004460_15	euxassay_004460_16	euxassay_004460_17	euxassay_004460_18	euxassay_004460_19

euxassay_004460_20	euxassay_004460_21	euxassay_004460_22	euxassay_004460_23	euxassay_004460_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7332_wholemount_strong.wlz

7332_wholemount_moderate.wlz

7332_wholemount_weak.wlz

7332_wholemount_possible.wlz

7332_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7332_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 08 09 10 11 moderate expression: see section 12 13 14 15 16 17

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 07 08 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 18 19 20

trigeminal v nerve

strong

regional

strong expression: see section 10

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 not examined expression: see section 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 14 15 16 17

cervical ganglion

moderate

regional

moderate expression: see section 10 18

thoracic ganglion

moderate

regional

moderate expression: see section 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6441
Entity Detected:	Reep5, receptor accessory protein 5 ( MGI:1270152)
Sequence:	sense strand is shown >T6441 GCCGCTCGCGATCTAGAGCCCAGCCATGTCCGCAGCCATGAGGGAGAGGTTCGACCGGTTCCTGCACGAG AAGAACTGCATGACTGATCTCCTCGCCAAGCTCGAGGCCAAGACCGGAGTGAACCGGAGCTTCATCGCGC TCGGTGTCATCGGACTGGTGGCTTTGTATCTGGTGTTCGGTTATGGAGCCTCTCTCCTCTGCAACCTGAT AGGTTTCGGATACCCAGCCTACATCTCAATGAAAGCCATCGAGAGTCCCAACAAAGATGATGACACCCAG TGGCTGACGTACTGGGTGGTATATGGTGTGTTCAGCATTGCCGAATTCTTCTCCGATCTCTTCCTGTCCT GGTTCCCCTTCTACTACATGCTGAAGTGTGGCTTCCTGCTGTGGTGCATGGCCCCCAGCCCGGCTAATGG GGCTGAGATGCGCTACAGGCGAATCATCCGTCCTATCTTCCTCAAGCACGAGTCCCAGGTAGACAGTGTG GTGAAGGACGTGAAGGACAAAGCCAAAGAGACTGCAGATGCCATCAGCAAAGAAGTCAAGAAAGCTACAG TGAACTTGCTGGGCGATGAGAAGAAGAGCACCTGAGGCCTGGCCGGGCGCCCCTCCCCCCCCACCCCCTG GAGCTCTGTGTCATGCTAGGGACAGTGGCACAGTCGTTGGATAATGTTGCCTTGGAAACTTCCCGCTGTA TTAAAAATGGAATGTGTTGTAAGCTGCTTTGCCTTTGTTGTCGTATATGTAGAAAGCATTTTCATTTAAA CCTAACAGAGTGGGCAGTACCTCAAGTGTTTAAAATATTTTGCTGTGTAGGAAGACATGTCACTATACTC CAGGAAATACAAAAGAGGGTTGGGAATCTCTGTCTGTAGGCCCCGTGCTCTGCGGGGCTCACTCTGCATG CGTCTCCTTGTGCTTACAGTTAGGAAACCTTTGTGGTTGTGCTTCACGCAGTGTATGATTATATATATAT ATAGTTCTGTGTTTTAATGTATCTGGGCATATACATATATATGGAAAATGTTATTGTTTGGCTAGAACAT GCACAGTGCTTGTGAGGAAGAGGCCAGGAGAGGCTTGTAGACGCCACCTGTGACCTGAGAAGGGAGACCA CCTGCCGAGGCTGGAGGTCTGAGCTTCCAATAACGTTTCAGAAATCAATGCAAGCGAGTAAGACTCGAAC CCGTGATCAGCTCCAGTTTCCCAGGAAACAGTGGCACCATCTCCCCTGCTCCTCCTCTGAGATGCTGAAA TGCCAGCCATTTATGGTTTACCTGAAAGCTGGCTAACAAGGTCCTTAGTCTCTCCTCCCAAACTCTTTAA AAACTAGTTCATGGCACCTCCCAAGACAGACATCCCACCTCCCATTTTTTAAAAACACGATCCCACGTAG CCCAGGATAACCTCCAAATCCCAGCTGCTGCTCATTGTAGCTTGTGCCCCACGGCTGGCATACGTGTGTG TTCTTGTTCCTGTCAGCAGCACATTGCGCTGATGACGTCCAGCTCTAAGAGCCATTCTGGGCAAGTGAAG GGCAGTGGCTAACAGCTGTTGTCGGTCCAGCAGTGGCTGGGTTAAACTTTCTACTCTTTTTTGGTTTTAT GGCCAACCTCAAGAGGTCTCCTTACTTTACAATTCATAATTATTAGCATTCCCTTAAAAATCTCGTTCAG TTTTAGGTTTTGTTTTGACAGATAGAATAAAGTTTAAGTGACTGCCCATGGCCTGATTGGTCAGAGGTTT GGGTCTAAAAATCTGCAGTTTGCACCATATGCCAGCGTCCCAGCCTTTGAGTTAGTCTGAAGTGGACTAA AGGAGTTGTCTACCAAAGTTAACAACAGGAGTTACTTTCAAGACAAATAGATAGTAATTCTCCTCCAGAT GACTAAGATCATAATAATATGATATTAAATATTAACAATACAAAATAAGAGGCACTGTGGCCCTAGCGTA ATATGGGTCACTGTGTCAGTTTTCCTGGGGATAGCAGTCATTAGGCTTCATATGACTTAGGGGAGATCTT TTTAGAAAATAAGAACTATTGTTTGGATAAAGTCAATTGTTAGGCTTTAAACATCAAAAATATTTAGGTC CTTATAAGTAAAAGCACGTTTTTCTTGTTGGCAGAATGAAAATTACAGTGTAGCATATAGGAGCAGAGTG TTAATATACAAAATACTTTCTGTGGGGAATGGGCAGGCAGGGAATGATGCTTACGGTTAGAGATTGTATT GGTGGAAACGCTGCAGTGAAATAAACTCCAAAAGTAATTTTGCTGCATCAACCCCACAACTTTGCGGGAG ACTCCCTGCTACTTTCTGCAGCTGTCAATGGGAGAAATCATGAATCGACTTCTGCCTACTGGTCCTTCCC AAATAAAGTGTCATGGCTTAAGTGTTGAAAATGAGCTGAGTTTGTGTCTGGCTGGAAACAAAGCATTTAT TTGTATCTGAGTGGTATTTCCTAGGCATATAAAGTTCTAGAATTCTTGCTAAACATTAAATGTCCCAAAG AGTTCGTGCAGCAATTATTTCCTGACATTGTGGGAAGGTCCTCCATCCACACCTGCAGAAAACAGCCAAG GCCTGTAAGGAAGTTCTTGCCTGTCCTCAGGTGTGAGTAGAAATGATGTGCCCAGTGCCTCCTTACACGT TCCCAGACTCCCCTGGCTGCCTGAACCTGGAAGAGTACTGAACTCTATGTGGACTGTTCTTTCCTGTGTG TGTACATATGTGACAAAGGTTACTGGCTGAACAAAGTGACAATTAGAAGAATTACAACATATACTCAAAT AAAGATTATGTTTATGTGGTCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3585290 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3585290 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7335 same embryo
	EMAGE:7333 same embryo
	EMAGE:7334 same embryo
	EMAGE:7337 same embryo
	EMAGE:7336 same embryo
	EurExpress:euxassay_004460 same experiment
	MGI:4827684 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source