EMAGE mouse gene expression spatial database

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EMAGE:7405

Rnf167 ring finger protein 167 ( MGI:1917760)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004443. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004443_01	euxassay_004443_02	euxassay_004443_03	euxassay_004443_04

euxassay_004443_05	euxassay_004443_06	euxassay_004443_07	euxassay_004443_08	euxassay_004443_09

euxassay_004443_10	euxassay_004443_11	euxassay_004443_12	euxassay_004443_13	euxassay_004443_14

euxassay_004443_15	euxassay_004443_16	euxassay_004443_17	euxassay_004443_18	euxassay_004443_19

euxassay_004443_20	euxassay_004443_21	euxassay_004443_22	euxassay_004443_23	euxassay_004443_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7405_wholemount_strong.wlz

7405_wholemount_moderate.wlz

7405_wholemount_weak.wlz

7405_wholemount_possible.wlz

7405_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7405_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7564
Entity Detected:	Rnf167, ring finger protein 167 ( MGI:1917760)
Sequence:	sense strand is shown >T7564 TTTTGTTGTTGTTGTTACCTGTTTGCCTTCGCTCCTATTCTGTCCTATTTCTGTACTAGAGAAAGATACA TTGTCTCCCCTACCAGCTCCGCTAAGCTGGTTTGATAGCTCACTCCCTGAATCGGAGTTCCTTCCCCGAC GCCCCCATGTAGCTGAGAAGTGGGACCTAGGGGTGGTTGGACCCCTGGGATCCTGAAGGAGAGGCAGAGG AGGGCGCAGGACTCGGCTTCTGCTCCTGCGATCAGAACGCGACCTTCGCAGCTTTACCCCTCCCGCTGCC ATGCACCCTGCGGCCTTCCCGCTTCCTGTTGTCGTGGCTACTGTACTGTGGGGAGCAGCCCCTGTCCGAG GGCTGATTCGAGCGACTTCGGAGCACAATGCCAGCATGGACTTTGCAGACCTTCCAGCTCTGTTTGGGGC CACTCTGAGCGACGAGGGACTGCAGGGGTTCCTTGTGGAGGCCCACCCAGAAAATGCCTGCGGTCCTATT GCCCCACCACCCTCAGCCCCGGTCAATGGGTCAGTCTTTATTGCACTGCTTCGAAGATTCGACTGCAACT TTGACCTCAAGGTCCTAAATGCTCAGAAGGCTGGGTATGGTGCAGCTGTGGTGCACAATGTGAATTCCAA TGAACTGCTGAACATGGTGTGGAACAGCGAGGAAATCCAACAGCAGATCTGGATCCCATCTGTATTTATT GGAGAGAGAAGCGCAGAGTACCTACGAGCCCTTTTTGTCTACGAGAAGGGGGCTCGGGTGCTTCTGGTTC CAGACAACAGCTTCCCCTTGGGCTATTACCTCATTCCTTTCACTGGGATTGTAGGATTACTGGTTTTGGC CATGGGAACAGTATTGATAGTTCGTTGCATCCAGCACCGGAAACGGCTTCAGCGGAACCGACTTACCAAA GAGCAACTGAAACAGATTCCTACCCATGACTATCAGAAAGGAGATGAATATGACGTCTGTGCCATCTGTC TGGATGAATATGAGGATGGGGACAAGCTTCGAGTACTTCCCTGTGCTCATGCTTATCACAGTCGCTGTGT GGACCCCTGGCTCACTCAGACCCGCAAGACCTGCCCCATCTGCAAACAGCCTGTACATCGGGGTCCTGGC GATGAGGAGCAGGAAGAAGAAACTCAAGAGCAAGAGGAAGGCGATGAAGGGGAGCCAAGGGACCAACCTG CTTCAGAATGGACCCCACTCCTGGGCTCTAGCCCTACTCTTCCCACCTCCTTTGGATCCCTAGCCCCTGC TCCCCTGGTCTTCCCTGGGCCCTCCACAGATCCCTCACCTCCTTCATCTGCTGCCCTGGCCTGACACCCC CATACTTGGTACCTCTGGTGACCGTCCTATTTGCACAAACTCTTTTTCCAGTCTAATAAGCTGAGGGTGA GGGATAGGGGACATTCTACCCCAGGTTCATGAACCCAGCCTCATCCTTTTGAGGGGGCTAGAAGCCATAG GGACATGGTTTTCATTTCTTGGATTAATAAAATTGGTTTTTTTTTGTGGATGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4241357 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4241357 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7404 same embryo
	EMAGE:7400 same embryo
	EMAGE:7401 same embryo
	EMAGE:7402 same embryo
	EMAGE:7403 same embryo
	EurExpress:euxassay_004443 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source