EMAGE mouse gene expression spatial database

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EMAGE:7409

Pcdhb4 protocadherin beta 4 ( MGI:2136738)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004728. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004728_01	euxassay_004728_02	euxassay_004728_03	euxassay_004728_04

euxassay_004728_05	euxassay_004728_06	euxassay_004728_07	euxassay_004728_08	euxassay_004728_09

euxassay_004728_10	euxassay_004728_11	euxassay_004728_12	euxassay_004728_14	euxassay_004728_15

euxassay_004728_16	euxassay_004728_17	euxassay_004728_18	euxassay_004728_19	euxassay_004728_20

euxassay_004728_21	euxassay_004728_22	euxassay_004728_23	euxassay_004728_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7409_wholemount_strong.wlz

7409_wholemount_moderate.wlz

7409_wholemount_weak.wlz

7409_wholemount_possible.wlz

7409_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7409_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 08 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 18 19

spinal cord

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 17 18

neural retina

moderate

regional

moderate expression: see section 01 02 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8696
Entity Detected:	Pcdhb4, protocadherin beta 4 ( MGI:2136738)
Sequence:	sense strand is shown >T8696 GTAGCATTCACTTTGGATCTAAGGAAGCGATCTCCAGAGAAGGCACAGCTCCAACGGGAAGCCAGCGAGT AAAATTTAGTTCTGCTATTTCATTAAAGCACGTGTGTGGATTCTAACACTTTAAAGACCCTGCACAGATT TGGATCCCCTGTGAACAATGGAGACAGCGCTAGCAAAAATGTCACAGCAAAGGCAAGTTGTTTTTCTTAC TATGTTGTTGCTTTTGTGGGTGTCTGGTTCAGAGGCAATTAAATATTCCATGCCAGAAGAAACAGAGAGT GGCTACTTGGTGGCTAACCTGGCACAAGATCTGGGGATCAGGGTTGGGGAACTGGCAGCTAGAGAGGCAC GAGTCCATCACAGAGGAAACAAAGACCTCTTGCAGCTGGATGCAGAGACAGGAAATCTGTTCTTGAAGGA AAAATTAGACCGTGAGGGGCTTTGCGGGGCGACAGAACCCTGTGTGCTGCACTTCCAGATCATACTGAAA AACCCTGTGCAGTTCTTTCAAACTGAACTGCAGCTCACAGATATAAATGACCACTCTCCGGAGTTCCCTG ACACGGAAATGCTCCTAACAATCCCTGAGAGCGCCCAGCCAGGAACTGTGTTTCCTCTGAAGGCAGCTCA GGACCCTGACATGGGGAGCAACGCTATTCAGAACTACACAGTCAGTCCCAACCTCCATTTCCATGTCGTT ACTCTCAGTCGCTCAGATGGCAGGAAATACCCAGAGCTGGTGCTGGACAGAGCCCTGGACAGGGAGGAGC AGCCTGAGCTCACTTTAATCCTCACCGCTCTGGATGGGGGAGCTCCACGAAGGTCTGGGATGACCACAGT TCATATTGAAATCATGGACATCAATGACAATGCTCCCCAGTTTGTACAATCGCTCTATGAGGTGCAGGTT CCCGAGAATTTCCCCCTTGATGCCTTACTTGTCACGGTCTCTGCTAGGGATTTAGATGCTGGGATATATG GCAATATAGCCTACTCTCTGTTTCAAGGTGATGGAGACTCCCAACCATTTGTAATAGATGAAATCACAGG AGAAATCCGTCTAAGCAAAAAGTTGGACTTTGAGGTAACTCCATACTATAATGTGGAAATCGCAGCCACG GATGGAGGGGGACTTTCAGGAAAATGCACTGTAGCTGTTCAGGTGTTGGATGTGAATGATAACGCCCCAG AGTTGACGGTCTCTACGCTCTCAAGCCCTATCCCCGAAAACTCCCCAGAGACTGTCGTTGCAGTTTTTGG TGTTTTTGACCCAGATTCTGGGGACAATGGAAGGATGGTGTGCTCTATTCAGAACGAACTTCCATTTCTT TTAAAGTCCACATTTGAGAACTATTATACTTTAGCGACAGAGAGGCCACTGGATAGAGAGAGTATCGCTG AGTACAACATCACCATCACTGTCTCAGACATGGGCACACCCAGGCTCACAACCCAGCATACTATAAAAGT GCAGGTGTCTGACATCAACGACAACGCCCCCGCCTTCACGCAAACCTCCTACACCCTGTTTGTCCATGAG AACAACAGCCCCGCCCTGCACATAGGCACCATCAGTGCCACAGACTCAGACTCGGGCTCCAATGCCCACA TCACCTACTCGCTTCTGCCGCCCCAGGACCCGCAGCTGGCCCTCTCCTCGCTCATCTCCATCAACGCTGA CAACGGGCAGCTGTTTGCGCTCAGGGCATTGGATTATGAGGCCCTGCAGGCCTTCGAGTTCCACGTGGGC GCCACAGATGGAGGTTCGCCCGCGCTCAGCAGCCAGACTCTGGTGCGCGTGGTGGTGCTAGACGACAATG ACAATGCGCCCTTCGTGCTCTACCCACTGCAGAATGCTTCTGCGCCCTACACCGAGCTGCTGCCCAGGGC AGCGGAGCCTGGCTACCTGGTCACCAAGGTGGTGGCAGTGGACCGCGACTCTGGTCAGAATGCCTGGCTG TCATTCCAGCTGCTCAAGGCTACGGAGCCCGGGTTGTTCAGCGTGTGGGCGCACAATGGCGAGGTGCGCA CCACCAGGCTGCTGAGCGAGCGAGATGCACCCAAGCACAGGTTGCTGTTGCTGGTCAAGGACAATGGAGA GCCTCTGCGCTCTGCCAGTGTCACAATGCACGTGCTAGTGGTGGATGGTTTCTCTCAGCCCTACCTGCCC CTGCCAGAGGTGGCGCTGGATCCTACACGGGAAGAAGATAATCTCACGCTGTATCTTGTCATCTCCTTGG CATCTGTGTCTTCTCTCTTCCTCTTGTCTGTGCTGCTGTTCATGGGGGCGAGGCTGTGCAGGAGGGCCAG GGAGGCCTCTCTGGGAGGCTACTCTGTGCCTGAGGGACACTTTCCTGGCCACCTGGTGGACGTCAGCGGG GCAGGGACCCTGTCCCAGAGCTATCAGTATGAGGTGTGTCTGAGCGGAGACTCTGGGATTACAGATTTTA AGTTCCTGAAGCCCAGCAATCCCAATAGTCTGATTCCAGACAATTACATGTTGAATAGTTAAAATTACAG AAAATTCTTAGTAATCAACAAGAGAAGGATAAGTCATAGTTGCTTGGATGTCGGTATTTTTCTGGTAATT AGTAATACTTCTTTGAGATTATGTTTTATTTCTATGTGCTTTTACTTTTTACATAATCAATTAATGCTTC AACTGCCTGCTGTCTTAGTAATGCAGTCTTAGCGTATATTTTCTGTCACTAGCAGGAAGTAGAAATCTTT ACTCTGCTAGTGATAACTCAAAGTTTTAAAATCTCATTTAATGTAGTGTATTTGACAAGCAATGTAGAAT GCCAAACATTTTCAAAATTTTATCTGTAACAGTTTTTATTTTGATTTTCTTAGAACGTACACTCACTGTA CAATGTGGTGGATTTGTTGTGGTGCTTGCATACATGTAGATTGTGTACTTTGATCATACTCATCCCTGAT CATCAGTCTCCGCCTATAATTTCACAATGTCCCTAAATATCACCTGGAAGTGAAAAATGTTAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6401988 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6401988 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7411 same embryo
	EMAGE:7407 same embryo
	EMAGE:7406 same embryo
	EMAGE:7410 same embryo
	EMAGE:7408 same embryo
	EurExpress:euxassay_004728 same experiment
	MGI:4827086 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source