EMAGE mouse gene expression spatial database

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EMAGE:7476

Ptprv protein tyrosine phosphatase, receptor type, V ( MGI:108027)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004621. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004621_01	euxassay_004621_02	euxassay_004621_03	euxassay_004621_04

euxassay_004621_05	euxassay_004621_06	euxassay_004621_07	euxassay_004621_08	euxassay_004621_09

euxassay_004621_10	euxassay_004621_11	euxassay_004621_12	euxassay_004621_13	euxassay_004621_14

euxassay_004621_15	euxassay_004621_16	euxassay_004621_17	euxassay_004621_18	euxassay_004621_19

euxassay_004621_20	euxassay_004621_21	euxassay_004621_22	euxassay_004621_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7476_wholemount_strong.wlz

7476_wholemount_moderate.wlz

7476_wholemount_weak.wlz

7476_wholemount_possible.wlz

7476_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7476_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cervical intervertebral disc

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 16

lumbar intervertebral disc

strong

regional

strong expression: see section 11 12 13 14 15

thoracic intervertebral disc

strong

regional

strong expression: see section 12 13 14 15

sacral vertebral cartilage condensation

strong

regional

strong expression: see section 11 12 13

axial skeleton tail region

strong

regional

strong expression: see section 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7612
Entity Detected:	Ptprv, protein tyrosine phosphatase, receptor type, V ( MGI:108027)
Sequence:	sense strand is shown >T7612 GGATAACCCTCAAAGTCGGGGGGCAACCCGTCACCTTCCTGGTAGATACTGGGGCCCAACACTCCGTGCT GACCCAAAATCCTGGACCCCTAAGTGACAAGTCTGCCTGGGTCCAAGGGGCTACTGGAGGAAAGCGGTAT CGCTGGACCACGGATCGCAAAGTACATCTAGCTACCGGTAAGGTCACCCACTCTTTCCTCCATGTACCAG ACTGCCCCTATCCTCTGCTAGGAAGAGACTTGCTGACTAAACTCAAAGCCCAGATCCACTTCGAGGGATC AGGAGCTCAGGTTGTGGGACCAATGGGACAGCCCCTGCAAGTGCTGACCCTAAACATAGAAGATGAGTAT CGGCTACATGAGACCTCAACAGAGCCGGATGTTTCTCTAGGGTCCACCTGGCTTTCTGATTTTCCCCAGG CCTGGGCGGAAACTGGGGGCATGGGACTGGCAGTTCGCCAAGCGCCTCTGATTATACCTCTGAAGGCAAG CTAACTATGGGACAGCCGCTAGTCATCCTGGCCCCCCATGCAGTAGAGGCACTGGTCAAGCAACCCCCTG ACCGCTGGCTATCCAATGCCCGCATGACCCACTACCAGGCAATGCTCCTAGACACTGACCGAGTTCAGTT CGGACCAGTGGTGGCCCTCAATCCTGCCACCTTGCTCCCTCTACCGGAGAAAGGAGCCCCCCATGATTGC CTCGAGATCTTGGCTGAAACGCATAGAACCAGACCGGATCTCACCGACCAGCCCATCCCAGACGCCGACC ACACCTGGTATACCGATGGGAGCAGCTTTTTGCAAGAAGGACAGCGAAAGGCTGGGGCAGCAGTGACGAC AGAGACCGAGGTAATCTGGGCGAGGGCCCTGCCAGCTGGAACGTCAGCCCAGCGAGCCGAACTGATCGCA CTCACCCAAGCCCTGAAAATGGCAGAAGGTAAGAAGCTAAATGTTTATACTGATAGCCGCTATGCCTTCG CTACGGCCCATGTTCATGGGAAAATATATAGGAGACGGGGGTTGCTGACCTCAGAAGGCAAGGAAATCAA AAACAAAAGCGAGATCCTAGCCTTGCTGAAAGCCCTCTTTTTGCCAAAGAGGCTCAGTATTATCCATTGC CCGGGACACCAAAAAGGGAACAGTGCTGAAGCCAGGGGCAACCAAATGGCGGATCAAGCAGCCAGGGAGG CAGCCATGAGAGAGAGCGCAGAAACGTCCACGCTCTTAATAGAGGATTCGTCCCCGTATACTCCCAGCTA CTTCCACTACACAGAGACTGACAAAAAAGACCTTCTGAGACTGGGAGCCGTATACGATGAGGACAAAAAG TATTGGGTCCTGCAGGGAAAACCTGTAATGCCTGACCAATTCACCTTTGAGCTCTTAGATTCCCTACATC GGCTCACCCACCTCAGCTATCAGAAGATGAAGGCACTCCTTGACCGGGAAGAGAGCCCCTACTACATGTT AAATCGGGATAGAACTCTCCAGCACGTAACGGAATCCTGTACAGCTTGTGCTCGAGTAAATGCTAGTAAA GCCAAAATCGGAGCAAGGGTGCGAGCGCGGGGACACCGACCCGGCACCCATTGGGAAATCGATTTTACCG AAGTGAAGCCAGGGCTGTATGGATATAAGTATCTCCTGGTGTTTGTGGACACATTCTCTGGCTGGGTAGA AGCATTTCCAACCAAGCGTGAAACTGCCAGGGTTGTAACCAAGAAGCTGCTTGAAGAAATTTTTCCAAGA TTCGGGATGCCCCAGGTATTGGGGACAGATAATGGGCCTGCCTTCATCTCCCAGGTAAGTCAGACAGTGG CCGATTTGTTGGGGATCGATTGGAAACTGCATTGTGCTTATAGACCCCAGAGTTCAGGTCAGGTAGAAAG AATGAATAGAACAATCAAGGAGACTTTGACCAAATTAACGCTTGCAGCTGGCACTAGAGACTGGGTACTC CTACTCCCCCTGGCCCTCTACCGAGCCCGGAATACTCCGGGCCCACATGGACTTACTCCGTATGAAATTC TGTATGGGGCACCCCCGCCCCTTGTCAATTTTCATGATCCTGAAATGTCAAAGTTAACTAATAGTCCCTC TCTCCAAGCTCACTTACAGGCCCTCCAAGCAGTACAACGAGAGGTCTGGAAGCCACTGGCCGCTGCTTAT CAGGACCAGCTGGATCAGCCAGTGATACCACACCCCTTCCGTGTCGGTGACGCCGTGTGGGTACGCCGAC ACCAGACTAAGAACCTAGAACCCCGCTGGAAAGGACCCTACACCGTCCTGCTGACCACCCCCACCGCTCT CAAAGTAAAATTGTTCGAATCAGGACAAGGGTGGTTTGAGGGACTGTTTAACAGGTCCCCATGGTTCACG ACCTTGATATCCACCATTATGGGCCCCTTGATAATACTTTTATTAATCCTACTCCTCGGACCCTGTACTC TCAACCGCTTGGTCCAGTTTGTAAAAGACAGAATTTCGGTGGTGCAGGCCCTGGTTTTGACCCAACAGTA TCACCAACTCAAATCAATGGATCCAGAAGCAGTGGAATCACGTGAATAAAAGATTTTATTCAGTTTCCAG AAAGAGGGGGGAATGAAAGACCCCACCATAAGGCTTAGCAAGCTAGCTGCAGTAACGCCATTTTGCAAGG CATGAAAAAGTACCAGAGCTGAGTTCTCAAAAGCTACAAGGAAGTTCAGTTAAAGATTAACAGCTAAAAA TCAAGGCTGAATAATACTAGGACAAGGGCCAAACAGGATACCGATGGTACCCACCTGGGCCCCGGCTCAA GGCCAAGAACAGATGATACCCACCTGGGCCCCGGCTCAGGGCCAAGAACAGATGGTTCCCAGACTCAGCT CGCTTCTCGCTTCTGTACCCACGCTTTTTGCTCCCCAGCCCCAGCCCTATAAAAAGGGTAAGAACTCCAC ACTCGGCGCGCCAGTCCTCCGATAGACTGAGTCGCCCGGGTACCCGTGTTCCCAATAAAGCCTCTTGCTG TTTGCATCCGAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30462835 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30462835 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7477 same assay
	EMAGE:7481 same embryo
	EMAGE:7479 same embryo
	EMAGE:7478 same embryo
	EurExpress:euxassay_004621 same experiment
	MGI:4825099 same experiment
	EMAGE:7480 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source