EMAGE mouse gene expression spatial database

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EMAGE:7504

Fam126b family with sequence similarity 126, member B ( MGI:1098784)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004583. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004583_01	euxassay_004583_02	euxassay_004583_03	euxassay_004583_04

euxassay_004583_05	euxassay_004583_06	euxassay_004583_07	euxassay_004583_08	euxassay_004583_09

euxassay_004583_10	euxassay_004583_11	euxassay_004583_12	euxassay_004583_13	euxassay_004583_14

euxassay_004583_15	euxassay_004583_16	euxassay_004583_17	euxassay_004583_18	euxassay_004583_19

euxassay_004583_20	euxassay_004583_21	euxassay_004583_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7504_wholemount_strong.wlz

7504_wholemount_moderate.wlz

7504_wholemount_weak.wlz

7504_wholemount_possible.wlz

7504_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7504_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7601
Entity Detected:	Fam126b, family with sequence similarity 126, member B ( MGI:1098784)
Sequence:	sense strand is shown >T7601 GGTAGGAAGCCTGAGGCTGCGGGGTGACAGAATTGGAGAATATTTAACTCTCAACAAATGAATTCCACAC TTGAACTCTGCCGCATTCCTGTGCCACCTCCTCCTTTAGAAAACTGATCCTAAGACAGAGATAAAAGAAG AATAGAAGGTAAAGGAAGATGCTGGGATCGGAACGAGCTGTTGTGGAAGAATGGTTATCAGAATTCAAGG CATTACCTGACACTCAGATTACCAGTTATGCAGCAACCTTGCACCGGAAAAAAGCCCTTGTACCAGCTCT CTATAAAGTTATTCAGGATTCAAACAATGAGCTCCTGGAGCCTGTCTGCCACCAACTGTTTGAGCTCTAT CGTAGCTCTGAAGTTCGACTTAAGAGGTTCACACTGCAGTTCCTGCCCGAATTGATTTGGGTTTATTTGC GACTTACAGTTAGTAGAGACAGGCAGAGCAATGGCTGCATTGAAGCACTTCTCCTAGGAATTTATAATTT GGAAATTGCTGATAAAGATGGAAACAATAAAGTGCTGTCTTTCACTATCCCTTCCTTGTCTAAGCCTTCA ATATACCATGAACCCTCAACAATTGGATCCATGGCTTTGACAGAAGGGGCATTATGTCAGCATGATCTCA TCAGGGTTGTTTACAGTGATCTTCATCCTCAGAGAGAAACATTCACTGCACAGAACCGGTTTGAAGTCCT AAGTTTTCTCATGCTGTGCTATAATTCAGCGATTGTGTATATGCCTGCATCATCTTACCAATCTCTTTGT CGGATGGGTTCCAGGGTTTGCGTGAGTGGGTTTCCACGACAACATGAGAAACAGTGGAAGGAGCTCTGTG GTCGAATTGTATTAGATCCCGAATTTATGGTGCGACTTCTCACAGGAGTTTACTATGCCATGTATAATGG ACAGTGGGACCTTGGCCAGGAAGTACTTGATGACATCATTTACAGAGCTCAGCTAGAACTCTTTTCTCAG CCACTCTTGGTGGCAAATGCCATGAAAAATTCATTACCATTTGATGCACCTGACTCTTCACAAGAAGGCC AGAAAGTACTTAAAGTGGAAGTCACTCCAACAGTGCCGAGAATTTCTCGGACTGCAATTACAACAGCTTC AATTCGTCGTCATAGATGGAGAAGAGAAGGTGCTGAGGGTCTAAATGGCGGAGAGGAGTCCTTAAACATG AATGATGCAGATGAAGGATTTTCATCAGGGGCTTCTCTTAGCAGCCAACCACATGGGACCAAACCACCCT CCTCTTCTCAGCGGGGAAGCTTGCGGAAAGTAGCAACTGGGCGTTCCGCCAAAGACAAAGAAACAGCATT AGCCATCAAATCCAATGAGAGCCCTCGAGATTCAGTAGTTGGAAAGCAGTTTGTACAACAACAAGCAGAT CTCAGCATAGATTCAGTTGAGCTGACACCGATGAAGAAACACTTGAGCCTGCCTGCAGGCCAGGTGGTGC CAAAAACCAATAGTCTGAGTCTAATCCGGACAGCCAGTGCTTCCTCAAGTAAATCTTTTGACTATGTAAA TGGCGGTCAAGCAAGTACCAGCATTGGGGTTGGCACTGAGGGAGTTACTAATCTAGCAGCTACAAATGCT AATCGATATTCAACTATCAGTCTACAAGAAGACCGGCTAGGTCACGCTGGTGAAGGTAAAGAGCTCCTCA GCCCAGGAGCCCCCTTAACCAAGCAGTCTCGATCCCCAAGTTTCAATATGCAGCTAATATCCCAGGTGTA GTTCTGATGCCCCTTGTTCTGCTAACCTTCTACACTGAACATTTCATTGTGCAAGACACGTCATCCCGCA TTGTGAAAAGATGTCACTGTCATCAGATTTGACTTAGCTTAGGTATCTCATACTGTATTTTGTATGGAAA CAGCAATAAGGTTTTCCTTTCCTTCCTTTTAACCTCTCTACCTATTCCCTTAAATGTGTTTGTGAAGCAG TGTAAAACGTTTGCCTTTTCCATGCCTTCCTTTCTCCTTGGGTGATGTGTAATCCATCGTAGGCTGATCG GTCCCATTTCAACACTGTGAGACTGAGGAGATGTCGAGGAAATGGTGTATAAGATCCCTATGAAATGATT CTTTGGCTTTTGTTTAAAAACAAAACGGGTTTGTTCACATAATCTATGAACTATGAAGATAACTGGATCA TATGTTTTCTCTCTAACCAGGAGTGCCTCAGAGATTCTGCTATGACTTTGGGCTTCTCTGAGCCTGTGCA ATTTTATATTCTTGGGAAGCATGGGGGAAAGTGGTAGACTACTGCAATTTTTTTATTAAAATGAGGCTAA CTGCCCCCCTTACTTTATCCCAAGGACACAAATGTTTAATTATTGAGGCATTTAAATCAATTTGTGACCA CCTCCATTGGAGGGTAGGGCTCTAAGTTGATTGTGTGATTGATAATGCACTTTCTCAATGGCTCTCTAGT CATTAACAACAGGTCTTCCCTCCTCCCACAAGGACTTAAGGACATTTCTGCCCTTAAAGTTTGAACTAAC AAATCAGAGACTCAAAGGGGCGTGTTCTATTTCCCAGGAATGATTGATATTATGGTGCTGGGGCTTTGTT GGGGGAAGGGTGTTGTTTGTTTAGTTTGTGTGAGATTGTAAATGAGGTGAAGTTATAATTGTTTTCTTTC TTCCTTTCCTTTGTGAGTTGGTGATGACTGAAGTAGACACTGTACGTCTTCAGGTAAAGAGAGGGATTTC TCAGTCCACTCTCTGAGATATCAGACTAGAGAAACCCTTTCAGCTGCTTCCTAGATGTACCTAGATTCAG TCAGATATGGGATACAGAAACCTAAAGTCCTGAGATCATATACTCCAAGGAAACACATCAGGGACAGGTG ATTGGTTGAAAACACTGATGCTTCTATGTGACACATTTGTATAGAACATTTCTACCAGAGGGTACTGACT TGATTGCTCTACAAGGACCACACTGCCTTTGTGTTTATTGCAGTTTGTGTATCTTCTGATTATCTGCAAA GTTTCTCGTTTTTATAAAACGTTGAAATCATGCCAGTAAACTAGATGTTGAATGTAAATGAGTAGCATTT GGTAACTGCTAAGAGGCTACAAAAATGCATTATTAGTTTAAAGGATTTTTGTTGTGATTTGGTTGGATAA GACTTTCTTATAATCTCTTCTGGGTCTCAGGCTTTGTTTGAAAAAGAAGGGAATATATTAGGATTTTAAA AATCAGTATTGTGTGACTTCAATTGCTATGAATTACCCATCTCAAGAACATCAGTTACACACACCCTTGT ACATGTAGACATTTTATGTATTGTGAATGAAAATTACACTGCCTATATAGGAAGCAAGTGTCCTCTTAAT TGACAAAGTTGTTTTATCTCTATTTACCGTAAAATTTGATAAATTAAATAGCCAGGAAAACGCCTCTCTT TTTTTTTTCATACATAAACCACCATTTTTGGAGGAGTTGTCTATCTGTCCATCTGTCTGTCTGCATTGTG TTAGAATAATCCTTAATTAGATTTGATAGTTTATAACATTTTTCAGCTCCCAGCAAATAGGGAACTAGAA CCTCAGCTATTATCCACATCATTGCCAAACTTTGGAGTAAAACTAAAAGCTGAAATGTGATCTATTGATT GTTTATAATATGCGTATGATATGTGTATCCATTTATCACAGACACATTTTAGCCCTATTTCTACTAATTT AACTAAGAGATATCAAGTAAACTTAAAAGAAAAAAGAATTGTAAAATAGTAAAATAAAATGTTTTTGTAA CCTACTGAACAGAGAGGCTTTGTGGTGCATAAGGAAGGTATCAGTCTTAGATGGCAAAGCACGCCTACTT CATGCTGGAGCTCACAGGCGCACATGTAACAGACTGGCATTTAAACCAAGCATTTTAAATAAAGCACAAG AGAATACCAAATAACTTGAAAGTATAGCATAAACTGTTGTAAATATGAGAAAAAATCTTTTGATTAGAAA CATCATAACTGAAGAGTTGGTTTAAGAATCAGGAATGGTGGCATACACCTTGAATCCCAGCACTCCAGAG GCAGAAGCAGGTGAATCTCTGAGTTCAAGGCTGGCCTGGTCTACAGATGGAGTTCTAGGACAGCCAGGGC TACACAGAGAAGGCCTGTCTCAGAAAAGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30431671 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30431671 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7502 same embryo
	EMAGE:7500 same embryo
	EMAGE:7503 same embryo
	EMAGE:7501 same embryo
	EurExpress:euxassay_004583 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source