EMAGE mouse gene expression spatial database

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EMAGE:7506

Cyp4a12a cytochrome P450, family 4, subfamily a, polypeptide 12a ( MGI:88612)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004560. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004560_01	euxassay_004560_02	euxassay_004560_03	euxassay_004560_04

euxassay_004560_05	euxassay_004560_06	euxassay_004560_07	euxassay_004560_08	euxassay_004560_09

euxassay_004560_10	euxassay_004560_11	euxassay_004560_12	euxassay_004560_13	euxassay_004560_14

euxassay_004560_15	euxassay_004560_16	euxassay_004560_17	euxassay_004560_18	euxassay_004560_19

euxassay_004560_20	euxassay_004560_21	euxassay_004560_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7506_wholemount_strong.wlz

7506_wholemount_moderate.wlz

7506_wholemount_weak.wlz

7506_wholemount_possible.wlz

7506_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7506_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

naris

strong

regional

strong expression: see section 08 09 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7539
Entity Detected:	Cyp4a12a, cytochrome P450, family 4, subfamily a, polypeptide 12a ( MGI:88612)
Sequence:	sense strand is shown >T7539 GAGTTGGTGATCCAGAAGCTGTTGTATCATGAGTGCCTCTGCTCTGAGCTCCATCAGATTCCCAGGAAGC ATCTCTGAGTACCTTCAAGTAGCCTCTGTGCTCAGCCTGCTCCTGCTGCTCTTCAAGACAGCCCAGCTCT ACCTGCACAGGCAATGGCTACTCAGCAGTACTCAGCAGTTCCCATCCCCACCTTCTCACTGGCTCTTTGG ACACAAGATCTTAAAGGACCAGGACCTTCAAGATATTCTAACTAGGATTAAGAATTTCCCAAGTGCCTGT CCACAGTGGCTCTGGGGAAGCAAAGTGCGCATTCAAGTGTATGACCCTGACTACATGAAGCTGATTCTGG GGAGATCAGACCCAAAAGCTAATGGTTCCTACAGATTTCTAGCTCCCTGGATTGGGCGTGGTTTGCTTCT GCTGGATGGACAGACATGGTTTCAGCACCGACGAATGTTGACCCCAGCTTTCCACTATGACATTCTGAAG CCTTATACGGAAATCATGGCAGACTCTGTTCGTGTAATGCTGGATAAATGGGAACAGATTGTTGGCCAGG ATTCCACCCTGGAGATCTTTCGACACATCACCTTGATGACCTTGGACACCATCATGAAGTGTGCCTTCAG CCACGAGGGCAGTGTCCAGTTGGACAGAAAATACAAGTCCTATATCCAGGCAGTTGAGGACCTGAACGAT CTCGTTTTTTCCCGTGTGCGGAACATCTTTCACCAGAATGACATCATCTACAGAGTGTCCTCTAATGGCT GCAAGGCTAACAGTGCCTGCAAACTTGCCCATGATCACACAGACCAAGTGATCAAATCAAGGAGGATTCA ACTTCAGGATGAGGAAGAGTTGGAAAAGCTTAAGAAGAAAAGGCGATTGGATTTCCTGGACATCCTCCTA TTTGCCAGAATGGAAAATGGAAAAAGCTTATCTGATAAGGACCTTCGTGCTGAAGTGGATACTTTCATGT TCGAGGGCCATGACACCACAGCTAGTGGTATCTCCTGGATCTTCTATGCTTTGGCCACAAATCCTGAACA TCAACAGAGATGCAGGAAGGAGATCCAAAGTCTCCTAGGAGATGGGACTTCTATCACCTGGAATGACCTG GACAAGATGCCCTATACTACCATGTGCATCAAGGAGGCCCTGAGGATCTACCCTCCTGTACCAAGTGTGA GCAGAGAGCTCAGCTCACCTGTCACCTTTCCAGATGGACGTTCTTTACCCAAAGGTATCCATGTTATGCT GTCCTTTTATGGCCTTCATCACAACCCAACTGTGTGGCCAAATCCAGAGGTGTTTGATCCTTCTCGATTT GCACCAGGGTCTTCCCGGCACAGCCACTCATTCCTGCCCTTCTCAGGAGGAGCAAGGAACTGCATTGGGA AACAGTTTGCGATGAATGAGCTGAAGGTGGCTGTGGCCCTGACCCTGCTCCGCTTTGAGCTGCTGCCAGA TCCCACCAGAGTCCCAATCCCCATACCAAGAATTGTGTTGAAGTCCAAGAATGGGATCCACTTGCATCTC AAAAAGCTCCAATAATCTTCACAGGACAAGACAGCTCAAATGGCATGCTGCCTGCCATTCTGTCTTTCTG TCACTTACTCTTTTCCCCAATCCTTCTGCTCACATCTCATTCTTTCTTCTCACCTTGTTCACCTCCACCC ACCTTCTGCTGGGCTTCCAGTCTCCTTGCCTGTCAGTCTTTTTCAACTTCTTCTGAGATCCCTACTTGCT TTTCTCTCTACCTGTCCCTAACCTGACTGCATGTTTGACCTTTGACCTTAATGATCTCCCTAACCTGCAC CCTGCCTTTCTCTTCTGTGTATTTCCTTCTCTTCTACCGCTTGTCTATCCTTAAATTGGGCTTATTCTGA TGTCATAATTAATTAAAAGATAATGTGTGCTCTTGTGATTCTAGATATCGAGTGTTTTTGATGATTTCAC TGGAATGAAGCTGAGGTCATTGCAGTGCCATCACTCCTTGGGTATCGCTGTGCCTCTTTTTACCTTTCTA TCACCTATAGCTGCATTCCTTGGTTTCAAGAGCCCTCCTAAGTGTTAAAATCCAACACCATTGTTAAGCA TCTCTTCTGTCTCCAGATCATTTCTCCATCTTTCCAATGTGTATGCTTGTTTTGTGCCTAAAAGGGTAAT ACTGGTCCTGTTCTTAGTTCTATCAAGATTAGGACATGCATATCTGAAAGAGCCAATTTTCAACCAACCT AACAGAGACCTTCCCTGACTGTCACCTCATCTATCCATATAAATCTCCCCTTCAAATTCTCCACCTATCA GCTTACGTTTAAGAGTCTTGCCATCTTCCCCACAATAAAGTTCATAATGTGGCATGTAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:4240359 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240359 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7509 same embryo
	EMAGE:7508 same embryo
	EMAGE:7510 same embryo
	EMAGE:7507 same embryo
	EMAGE:7505 same embryo
	EurExpress:euxassay_004560 same experiment
	MGI:4824177 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source