EMAGE mouse gene expression spatial database

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EMAGE:7515

Crbn cereblon ( MGI:1913277)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004586. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004586_01	euxassay_004586_02	euxassay_004586_03	euxassay_004586_04

euxassay_004586_05	euxassay_004586_06	euxassay_004586_07	euxassay_004586_08	euxassay_004586_09

euxassay_004586_10	euxassay_004586_11	euxassay_004586_12	euxassay_004586_13	euxassay_004586_14

euxassay_004586_15	euxassay_004586_16	euxassay_004586_17	euxassay_004586_18	euxassay_004586_19

euxassay_004586_20	euxassay_004586_21	euxassay_004586_22	euxassay_004586_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7515_wholemount_strong.wlz

7515_wholemount_moderate.wlz

7515_wholemount_weak.wlz

7515_wholemount_possible.wlz

7515_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7515_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 16 17 18 weak expression: see section 07

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 08 16 17 18 19 20 21 22 weak expression: see section 07

dorsal root ganglion

moderate

regional

moderate expression: see section 15 16 17 weak expression: see section 07 08 09 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7668
Entity Detected:	Crbn, cereblon ( MGI:1913277)
Sequence:	sense strand is shown >T7668 AAGAGGTAATCCCCGGCGAGGGAGATCAGCAGGACGCTGCGCACAACATGGGAAACCACCTGCCGCTTCT GCCTGACAGTGAAGATGAAGATGATGAAATTGAAATGGAAGTTGAAGACCAAGATAGTAAAGAAGCCAGA AAACCGAATATCATAAACTTTGACACCAGTCTGCCAACCTCACATACATACCTGGGAGCTGATATGGAGG AGTTCCACGGGAGAACTTTGCATGACGACGACAGCTGCCAGGTGATCCCAGTCCTTCCTGAGGTGCTGAT GATCCTGATTCCTGGGCAGACACTCCCACTGCAGCTCTCTCACCCACAGGAAGTCAGCATGGTGCGGAAC TTAATCCAGAAAGACAGGACCTTTGCAGTCCTTGCATACAGTAATGTGCAAGAAAGGGAAGCACAGTTTG GGACAACAGCAGAGATCTATGCCTATCGAGAAGAGCAGGAGTTTGGAATTGAAGTAGTGAAAGTGAAAGC AATTGGAAGGCAGCGGTTCAAGGTCCTCGAACTTCGAACACAGTCAGATGGAATCCAGCAAGCTAAAGTG CAGATTTTGCCAGAGTGTGTGTTGCCGTCAACCATGTCTGCAGTGCAGGTAGAATCACTCAATAAGTGCC AGGTATTTCCTTCAAAACCCATCTCCTGGGAAGACCAGTATTCATGTAAATGGTGGCAGAAATACCAGAA GAGAAAGTTTCACTGTGCAAATCTAACATCATGGCCTCGCTGGCTGTATTCATTATATGATGCTGAAACA TTAATGGATAGAATTAAGAAACAGCTACGTGAATGGGATGAAAATCTCAAAGATGATTCTCTTCCTGAAA ATCCAATAGACTTTTCTTACAGAGTAGCTGCTTGTCTTCCTATTGATGATGTATTGAGAATTCAGCTCCT TAAAATCGGCAGTGCTATTCAACGGCTTCGCTGTGAATTGGACATCATGAACAAATGTACTTCCCTTTGC TGTAAACAATGTCAAGAAACAGAAATAACGACAAAGAATGAAATATTTAGTTTATCCTTATGTGGTCCAA TGGCAGCATATGTGAATCCTCATGGATATGTACATGAGACACTGACTGTGTATAAAGCGTCCAACCTGAA TCTGATAGGCCGGCCTTCTACAGTGCACAGCTGGTTTCCCGGGTATGCATGGACCATTGCCCAGTGCAAG ATCTGTGCAAGCCATATTGGATGGAAATTTACAGCCACAAAAAAAGACATGTCACCTCAAAAATTTTGGG GCTTAACTCGCTCTGCTCTGTTACCCACAATTCCAGAGACTGAAGATGAAATAAGTCCAGACAAAGTAAT ACTTTGTTTATAAGTGCACCTGTAGGAGTGACTTCCTGACAGATATTTCCTCAAGTCAGATCTGCCCAGT CATCACTGCCTCTGATATATGTGTATAGTGGGTTACAGCATTTGCCTACCAAGTTCAAGAGCATATTTAG GGAATGAGAAAGCAGTATAAAACATAAGGCTGGGTTCCAAAATACTTGCTTTTTAGTAGCTTGGTGCCAT GGATTATCCTGTTGAGTCTATGTCATGACAGGATAGGAAAACACAGTTGAAATAATGGGAATGGCCATGG AACAGGATAGGGGCACCACTGCTCTAAATGATGAAGCTCTAAATGATGAATGCTCCAGAAACTGGGTTGG TAAGCACAAGATAGAGGCAAGGCAGTGTAATTTTAAAAGGACTTTGCTCCTTTCAATTTTCCTTAGCTTG TCTGAGATACTGACCTGTACATTTTGAACATATTAAAGAGTAACTAAGTATTCTGAGCAGAAATAGCAGC ATTTGGTGTAGTTGCACTTTTGATTTGATGAGCCTGTGATGTGCTAGATCCCTTTAACTAATGTATATGT CCATTTTGCATTTTATTTGCAAATATAAGTGAACAGTATATATTTCTAGGATTATACCATTTAGGAAACA GGTTTACATAAACATAAATATCCAAATCTATTCTATTTGGCTGAATTATGTCAAAGTAATCAAGTAGAAT ACTGAAAGGTGTAAGTACGTAATAAAATGCAACTCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30443656 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30443656 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7516 same embryo
	EMAGE:7514 same embryo
	EMAGE:7512 same embryo
	EMAGE:7513 same embryo
	EMAGE:7511 same embryo
	EurExpress:euxassay_004586 same experiment
	MGI:4824042 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source