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EMAGE:7573

Dppa5a developmental pluripotency associated 5A ( MGI:101800)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7573 EMAGE:7573 EMAGE:7573 EMAGE:7573 EMAGE:7573
"Pseudo-wholemount" of euxassay_004611. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004611_01 euxassay_004611_02 euxassay_004611_03 euxassay_004611_04
EMAGE:7573 EMAGE:7573 EMAGE:7573 EMAGE:7573 EMAGE:7573
euxassay_004611_05 euxassay_004611_06 euxassay_004611_07 euxassay_004611_08 euxassay_004611_09
EMAGE:7573 EMAGE:7573 EMAGE:7573 EMAGE:7573 EMAGE:7573
euxassay_004611_10 euxassay_004611_11 euxassay_004611_12 euxassay_004611_13 euxassay_004611_14
EMAGE:7573 EMAGE:7573 EMAGE:7573 EMAGE:7573 EMAGE:7573
euxassay_004611_15 euxassay_004611_16 euxassay_004611_17 euxassay_004611_18 euxassay_004611_19
EMAGE:7573 EMAGE:7573 EMAGE:7573 EMAGE:7573
euxassay_004611_20 euxassay_004611_21 euxassay_004611_22 euxassay_004611_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7573Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7573_wholemount_strong.wlz
7573_wholemount_moderate.wlz
7573_wholemount_weak.wlz
7573_wholemount_possible.wlz
7573_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7573_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
testis
strong strong
regionalstrong expression: see section 04 05 moderate expression: see section 19 weak expression: see section 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7684
Entity Detected:Dppa5a, developmental pluripotency associated 5A ( MGI:101800)
Sequence:sense strand is shown

>T7684
AAGAGGTAATCCGGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCTTGGCAGGATGATGGTGACCCTCGT
GACCCGTAAAGATATCCCCCCGTGGGTGAAAGTTCCTGAAGACCTGAAAGATCCAGAAGTATTCCAGGTC
CAGTCGCTGGTGCTGAAATATCTGTTTGGCCCACAGGGATCTCGAATGTCTCACATCGAGCAGGTGAGCC
AGGCCATGTTTGAGCTGAAGAACCTGGAATCTCCCGAAGAACTTATCGAGGTCTTCATTTACGGCTCTCA
AAACAACAAGATTCGGGCTAAATGGATGCTTCAGTCCATGGCTGAGAGGTACCACCTGCGCCAGCAAAAA
GGAGTGCTGAAGCTGGAGGAATCCATGAAGACCCTGGAGCTAGGCCAGTGTATCGAGTGAAGCCAGTTTC
CAGTCCTTGTGTCTCCGACCTGGATGCAGGTTAAGCTGTGGCCAGTGTTTGGTTCTGGCGGGATTTTTAG
CTTTGTTACATCCTAGCAAGATATTCCTGGATCCCTGCTGCGCATTCTGATGTGAATCCCAAGGTTACCA
CTCTAAATAAAAAATAAAATTGAAGTGATTAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30732471 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30732471 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7574 same embryo
 EMAGE:7575 same embryo
 EMAGE:7578 same embryo
 EMAGE:7576 same embryo
 EMAGE:7577 same embryo
 EMAGE:7572 same embryo
 EurExpress:euxassay_004611 same experiment
 MGI:4824387 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS