EMAGE mouse gene expression spatial database

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EMAGE:7587

Zhx2 zinc fingers and homeoboxes 2 ( MGI:2683087)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004709. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004709_01	euxassay_004709_02	euxassay_004709_03	euxassay_004709_04

euxassay_004709_05	euxassay_004709_06	euxassay_004709_07	euxassay_004709_08	euxassay_004709_09

euxassay_004709_10	euxassay_004709_11	euxassay_004709_12	euxassay_004709_13	euxassay_004709_14

euxassay_004709_15	euxassay_004709_16	euxassay_004709_17	euxassay_004709_18	euxassay_004709_19

euxassay_004709_20	euxassay_004709_21	euxassay_004709_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7587_wholemount_strong.wlz

7587_wholemount_moderate.wlz

7587_wholemount_weak.wlz

7587_wholemount_possible.wlz

7587_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7587_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8656
Entity Detected:	Zhx2, zinc fingers and homeoboxes 2 ( MGI:2683087)
Sequence:	sense strand is shown >T8656 GTGGGGGGAGCCAGCAGAGTTCCATTTTGGAACGCCCGTGCCTCGTCTCCGCGTTCCCAGCCCGGGTCCC CGCGTTCGCAGCCCCGGCGCAGGTCTCCATGCACTGGCTGCTCTGGGAATCTGGCTGCAAGATCCCCAGG ATGTAAGGAACTCACAGCAGACACTGATAGGAAGCTGCCTGGTTCGCTCAGAGATTGCTACAGTTACAAT TGTCTGTGCTGAAATCCTTCTGAAACACTCACCCGGAAGACGTCAGCAACCAAGGGAAGCCAAAGGCATT TGAGAGGGAGTAGAGCCAAAAGCTGAGAATCTCACTGTCCTGCACCCACCCACCCACTCCAGAAAGAAGC TGTTGCTCAGAGGCAGACGGCATGGCAAGCAAACGGAAATCTACAACTCCCTGCATGGTTCGGACATCAC AGGTACTGGAACAAGATATGCTGGAGGAGGCAGACAGGGCCAAAGACAAAGGAGCGGGCATGCCACAGTC CGATGTGACAAAGGACAGCTGGGCAGCGGAACCTGAACATTCGTCCAAAGAAACCGAAGTGGTTGAGGTG AAATCTATGGGGGAGAACCTGTCCAAAAAACTACAGGGCGGTTATGAGTGCAAATACTGCCCTTATTCCA CGCAAAATCTGAATGAGTTCACAGAACACGTGGACATGCAACATCCTAACGTGATTCTCAACCCCCTCTA CGTATGTGCCGAATGTAACTTCACAACCAAAAAGTACGACTCCTTGTCTGACCACAACTCCAAGTTCCAT CCGGGGGAGACCAACTTCAAGCTGAAATTAATCAAGCGCAATAATCAGACGGTTCTAGAACAGTCCATCG AAGCCACCAACCACGTCGTGCCCATCACTGCCAGTGGTCCTGGAAGTAGTGATAACGACCCTGGGGTCTC GGTAGGAAAAACCCCCATGACAAAGACAGGAAAGCTGAAGGCAGATGCCAAGAAAGTGCCCAAGAAGCCT GATGAGGCTGCCCCAGAAAACCACATGGAAGGGACCGCCCGCCTGGTGACAGACACAGCTGAGATCCTCG CCAGACTTGGTAGCGTGGAGCTCCTTCAAGATTCACTGGGACATGTCATGCCTTCCGTACAGCTGCCACC AAATATCAACCTTGTCCCCAAGGTCCCCGTCCCGCTGAATACTACCAAATACAACTCTGCCCTGGACACA AATGCTACCATGATCAACTCCTTCAACAAGTTCCCTTACCCCACCCAGGCTGAGCTCTCCTGGCTGACGG CGGCCTCCAAACACCCAGAGGAGCACATCCGAATCTGGTTTGCCACCCAGCGTTTAAAGCACGGTATCAG CTGGTCCCCAGAGGAGGTGGAGGAGGCCCGGAAGAAGATGTTTAACGGCACGATTCAGTCAGTACCCCCG ACTATCACTGTGCTTCCTGCTCAGCTGACTCCCACAAAAGTGTCACAGCCCATCCTCCAGACAGCCCTAC CATGCCAGATCCTTGGCCAGCCCAGCCTGGTGCTGACTCAAGTGACAAGCGGGTCAACAACTGTCTCCTG CTCTCCCATCACACTTGCGGTGGCTGGGGTGACCAATCATGGCCAGAAGAGACCTCTGGTGACTCCTCAA GCTGCCCCTGAGCCGAAGCGTCCACACATTGCCCAGGTGCCAGAACCTCCACCCAAGGTGGCCAATACCC CGCTCACCCCAGCTAGCGACCGCAAGAAGACCAAGCTACAGATAGCTCACCTCAAGGCAAGCTTCTTACA GAGCCAGTTCCCTGACGATGCTGAGGTCTACCGGCTCATTGAGGTGACAGGCCTTGCGAGGAGTGAGATC AAGAAGTGGTTCAGTGACCACCGCTACCGGTGTCAGAGGGGCATCGTCCACATCACCAGCGAATCCCTTG CCAAAGACCAGATGGCCATTACTGGCACCCGGCACGGCCGCACCTACCATGTATACCCAGACTTTGCCCC GCAGAAGTTCAAAGAGAAAAGCCAAGGACAGTTGAAAACCCTGGAAGACAGCTTTCTAAAAAGCTCCTTT CCCACCCAGGCAGAAGTGGAACGGCTGAGGGTGGAGACCAAGCTGAGCAGGAGGGAAATCGACTCCTGGT TCTCAGAGAGGCGGAAGCTTCGAGACAGCATGGAGCAGGCTGTGTTGGATTCCATGGGGTCTGGCAAAAA GGGCTCAGACGCGGTAGCCCCCAATGGTGCTCTATCCCGACTTGATCAGCTCTCTGGTGCCCAGTTAGCT GGTTCTTTGCCCAGTCCTTCATCAGCAATTGTACAAAATCAAGAACAGGTTCACCTGCTCAGGAGCACTT TTGCAAGAACCCAGTGGCCCACTCCTCAGGAGTACGATCAGTTAGCTGCCAAGACCGGCCTGGTCCGAAC TGAGATTGTGCGCTGGTTCAAGGAGAACAGATGCTTACTAAAAACTGGAACGTTGAGCTGGCTGGAGCAG TACCAGCGGCATCACATGTCAGATGACCGTGGTCGTGATGCCGTATCAAGAAAGGTGGCAAAACAAGTTG CTGAGAGCCCAAAGAATGGAAGCGAGGCGGCACATCAGTATGCCAAGGACCCCAAAGCACTCAGCGAGGA GGACTCCGAGAAGCTGGTCCCGAGGATGAAAGTGGGTGGTGATCCAACCAAGGACTGTTTGGCAGGCAAG CCCTCAGAGGCCACCTCAGACAGATCAGAGGGCAGCCGAGATGGCCAGGGCAGTGAGGAGAACGAGGAGT CAGGCATCGTGGACTTTGTGGAGGTGACAGTTGGTGAGGAGGACGCCATCTCAGAGAAGTGGGGTAGCTG GAGCCGGAGAGTAGCAGAAGGCACAGTGGAGAGGGCAGACTCCGACTCCGACAGCACCCCTGCAGAGGCC GGCCAAGCCTAGACAGGGCATCGGGTCTGAACCTCCGCACTGACGAGGGAGGTGCTCTGTCTTTGAAGAG AGAAGAAAGCCCCGCCCCAGTGGCTGTGGGCCACCTCACCAGCGACTCTGAGCAGGCGTGCTTCAGTGGT GCCTGGAGGCCACAGGAACTCCGGAGCTCTCAATGTCCCTCCCAGAGGCTGGGTGGGAGTTGTTCATAGT GCCAAAGTCCTACTACTGCGTTTTCAATGGGTCCCTGTGCGTAGCCTTTGCCTTCGCCCCAGCCCCGCCT CTTGCTATACTGGAACTGATTTATACAATGTGGGAATTTTGTTACCTTTTTAATCGAGGGCAACTTCCTT TTTCCAGCACTTCTGTGGTAAGGCGTTTGCATTTTGTTTTGTTTTGTTTTGTTTCACCCCCAGGAAGGAG GGCTAACCACATTGCTTTTCTTTTCTCTTTCTTCCTTTCACTTTAGTAACGAGGGGGAGGGAGCATTAGC ATAAGTACCACTGTATCTACTGGATTTTAGGTAGGACGGATTTCTTCTTTAAGGTAGTTTGACATGGGAG ATGCCTTGGTGGGAAGGGCTGACGTCTGGCGCAAAGTGGGATGAGCTCACAAAGAGCCTTCCCCATCAAA CCCTTCCCAGTTCCCCAGCTGCCTTTTGGGGACCCTGCCTCAGCTGGCTTCTCAACTGCCTTGGATAATT GGGGAACAGGTGGTTGCCCTCTTTACAAATCGTTTTGAAATACCAGCAGAAAGGGCTCCCACAACACTTG ATGTCACCATCACTCTTGGGAAATTAAGAGAGTGGTCTTCCCGGCCTAGGTGTAGTACCGAGGTAAACAT CCCTGCTTTTCTTTTCTCTTCTCACCACCTTTTCTTGGTGTGGCTTTTGGCAAGATTTCAGCTCAAAGCC TCACTAATGGATTGATTTGTTGTTGTTGTTGTTCCTGTGTCTGTGTGTGTGTGCGCGCGTGCGTGCGTGT GTTGGGCCAATTTTAGATACCTGAGTGCACTTTTTTTTTTTTTTTAGTTAGTCCTAGCTTTTAAAGAAAG AAAGCCAAGAGATCTAGTTGGTGTAAATGTGGAGACAGGGATTCTGTTCGCTGTCCTTGTCCCCTCCCCC ACCCCGCAGACCCCCGACCCCACTGGAGTACACTGCACAAATTAAATGCTAGGGAGTTTGAATAAAAGCA GTTTTTCTAACTTGTTGCTCATCTGTTGTAACTCAATAAAGCAAAGACTAAACGTTTTTCTAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6400319 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6400319 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7588 same embryo
	EMAGE:7586 same embryo
	EurExpress:euxassay_004709 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source