EMAGE mouse gene expression spatial database

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EMAGE:7663

Tmem150c transmembrane protein 150C ( MGI:3041258)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005300. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005300_01	euxassay_005300_02	euxassay_005300_03	euxassay_005300_04

euxassay_005300_05	euxassay_005300_06	euxassay_005300_07	euxassay_005300_08	euxassay_005300_09

euxassay_005300_10	euxassay_005300_11	euxassay_005300_12	euxassay_005300_13	euxassay_005300_14

euxassay_005300_15	euxassay_005300_16	euxassay_005300_17	euxassay_005300_18	euxassay_005300_19

euxassay_005300_20	euxassay_005300_21	euxassay_005300_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7663_wholemount_strong.wlz

7663_wholemount_moderate.wlz

7663_wholemount_weak.wlz

7663_wholemount_possible.wlz

7663_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7663_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 09 10 13 14 15 16 17 18 weak expression: see section 02 03 04 05 06 19 20 21

thalamus

moderate

regional

moderate expression: see section 05

thalamus mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 11 12 13 14 15

telencephalon marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 03 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 15 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

ventral grey horn

weak

regional

weak expression: see section 07 08 09 10 11 12 13

dorsal root ganglion

weak

regional

weak expression: see section 06 07 08 09 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8942
Entity Detected:	Tmem150c, transmembrane protein 150C ( MGI:3041258)
Sequence:	sense strand is shown >T8942 GCGGCCTTGAAGTCCTCCGTCCATTATCAGGGTCAGAAGTTAGCCCTGTGTCTGCTCAGTAAGATGAAAG AAAACCACGGGAGGGCAGCGGGTGGTGGAGAACTACGCTGGGCTCGTGGGCCTGAAAGTGAATTTTAGCT GCTGTATGCAAAGTCCTTGGTGTGTGGACAGAAGGGAGCCGGTAAGAAGGTTACAACAGGTCAGGAATAA ATAACCAGCAGGCGGAGGATCCCGGATCCCGTCCTGCCTTTTGTTGGGTGAGGAGCGCCAGGCAAGCGCG TCCACCTTGGCTTAGGAAGGAGGCGATTGGACCGTCCGCCCGCAGCCGCAGCCCCGGGTGACGTTGCACA GGCATGGACGGGAAGAAATGCAGCGTGTGGATGTTTCTACCTCTCGTGTTTACTTTGTTTACTTCAGCTG GCTTGTGGATAGTGTACTTTATAGCCGTGGAAGATGACAAAATTTTGCCATTAAATTCAGCTGCAAGGAA ATCGGGTGCGAAGCATGCTCCATACATCAGTTTTGCAGGTGACGACCCTCCTGCGAGCTGTGTGTTCAGT CAAGTAATGAACATGGCCGCCTTCCTAGCCCTCGTGGTAGCTGTTCTGCGCTTCATCCAGCTAAAACCAA AGGTTTTAAACCCATGGCTGAATATCAGCGGTCTGGCGGCGCTGTGTCTGGCTTCCTTTGGAATGACCTT ACTTGGTAATTTCCAGCTCACAAACGATGAAGAAATCCACAACGTGGGGACATCCCTGACCTTCGGGTTT GGCACTCTGACCTGCTGGATCCAGGCCGCACTGACACTCAAAGTCAATATCAAGAACGAGGGGCGGAGAG CTGGGATCCCGAGAGTCATCTTGTCAGCTGTCATTACCCTCTGCGTAGTCCTGTATTTCATCCTGATGGC CCAAGATATCCACATGTACGCAGCCAGGGTCCAGTGGGGCCTGGTCATGTGCTTCCTGGCGTACTTTGGC ACCCTGGCTGTGGAGTTCCGGCACTACCGCTATGAGATTGTGTGCTCCGAGTACCAAGAGAACTTCCTGA GCTTCTCAGAAAGCCTGTCAGAAGCTTCTGAATATCAGACGGATCAGGTGTAGCAACAGTTTGTCCTTGG GGGTGCGGTGGGTGTGGCAACAGGGGAGGGGCCAGGAGGATACACTCACGGGACTCGACACATCACCTCA CGATGCTTTTCACACACACACACACACACGGATGCACACTCACGGCCATATCTGACAGAAGAGCTTTTCA GGGCAGGTTACTTCTTTCTTGAACAAGCACAAGCCCTCATGTATGGAAATACACGCTGTCACACCGAAAA CATGCACGACAGAGCCAGAAGCTTGTGCATGACCACCTCCCCGCCCCCCACGCCCTCCCAGCAGGCCTTG CGCTTCTCATCCTTTTCCCGGTTCCAACCTGGCATCTTCCTCCTGCAAAGTAAGGCCGGCCGAATGTAAA GTGAGAGTCAAGCCAGCTCCCTAAGTCGCCTTCAGGTTGGAAGGGTTTGAGGCGAGCACGAGGGGAACTT GAGTGGAAACCTAGGAGCATCTGGCTGCATGCACCCCGCACTAAAGCTGGCCGTGGCCTGAGAGGCGTGG GCAGAGCTTTCCCATCAGTCTGACATCATCACTGTTCAGCGGTCGGCTTTGTGGATCCAGAGAGAGAAGC ATGCTACAGAGAGTGAGCGGGAAAGAAGCCGGCCACCAGCATTTGGACTTGAGTCACTCTGCCTGAGGAA GATCTCCAAACGTGAATGCTACCGGGGACAGTCAGCCGCGAATGGCACGAGGTACGGCTGAGAGGGCGTG GCTTGGTTTTTCCTTCCACAGTGCATCACCCACAGTTCCAGTTTGCATTTTCTTTTTGAAGTGCCACTCT CACGGGTCTATGTAGGCCTCGTGGGCAAAGGTGTGGACCAAAGGCAAGAACCTTGTTTTGGCTTCAAGTA TGAAAGGGGTATACGTGCAAGCTTAATTTATATCTGAAGAAATAATAAGAAAAAAAGATTCTCGAAGAAT CGCAAAAGAAATCTACATGGAGGTGAAAGGTATGAGGGCTCTGAGGAAAGACAGGAGGTTAGCCCTCTTG CTGCTAGTCCAACCCAGGGGAGATTTTAACATCCGTGTGTTGCTCAAATTTTATAGTTTGAGGACTGGGG ACTTTAAATTAAATTTGGAAGTTATAGAAGCAAATTAAAAACATCGCCCTCCCGCAAATCACATTGGCAC AAATGGAGCTGCAATACTGCTAAGCTGTTCGGAGATGAGCCCAGAGGTGCAGGAGTTCAAGGCCATCCTA GATCCCTGTGCCAACAACAGAGTGCCAAGTGCACAAGCACCCCCTCCCCCAACCAAGTGCACAAGCACCC CCTCCCCAACCAAGTCACAAGCACCCCCTCCCCAACCAAGTCACAAGCACCCCCTCCCACATTGTTTCAA AAAACTCTCCAAACTCCTAGGGGGCTCAGACAGCCCGGAATGTAATGTGGAAAACGTTGTTTTGTAAATT CACCCTGAAGTATAGCTTCCCAAGGAAGGGTTCAAATGCTAAATCTCCGCCCCCCAGCATAGCGCCGAGC ACGAGCACACAGTAAATGTCCAAATGAACAGATTTGCTGCTCGTTTCTGAGTGGAGGTGAGGAGCATCAA AGAGATTCAGAGACAAACGACAGAGTGGAAGACAGAGTGAAGAAGGAGGGGGAGGACTCATCACTACAGG GCAGGGCAGGGCAGGGAGTGAAAAGCCCCCAGTGGTGTGAGAAAGCCTCGAGTAGAGTATTCTCTTATTA AAAAAGGCGTCTTACCGTAAATACTCCAGGGGGATGTCTGTTAGTTTTGAAACTGCTATGAGGTTTCTCT TCTTTCCTCCTTTTCACTTTTCTATTTTAAAGATTTTTATCAAAGAACAAACGGTTTTATTTTACTCTCC ACACTCCAAGTAAAGAGCTTTCGGTGACACACTTCTCTGCGTTCTTAAGAGTGTGCAGCAGCAGCAGCGG CAGCAGCAGCATCCAGCCAATACTGACATTACTCACTGGGACCTGGCCACTGAATAAAGGGGTTTTATGC TAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6419443 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6419443 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7662 same embryo
	EMAGE:7664 same embryo
	EMAGE:7665 same embryo
	EurExpress:euxassay_005300 same experiment
	MGI:4828780 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source