EMAGE mouse gene expression spatial database

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EMAGE:7678

Scyl3 SCY1-like 3 (S. cerevisiae) ( MGI:1921385)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005290. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005290_01	euxassay_005290_02	euxassay_005290_03	euxassay_005290_04

euxassay_005290_05	euxassay_005290_06	euxassay_005290_07	euxassay_005290_08	euxassay_005290_09

euxassay_005290_10	euxassay_005290_11	euxassay_005290_12	euxassay_005290_13	euxassay_005290_14

euxassay_005290_15	euxassay_005290_16	euxassay_005290_17	euxassay_005290_18	euxassay_005290_19

euxassay_005290_20	euxassay_005290_21	euxassay_005290_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7678_wholemount_strong.wlz

7678_wholemount_moderate.wlz

7678_wholemount_weak.wlz

7678_wholemount_possible.wlz

7678_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7678_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8922
Entity Detected:	Scyl3, SCY1-like 3 (S. cerevisiae) ( MGI:1921385)
Sequence:	sense strand is shown >T8922 CGGAGAGCCGCCGGAGCCGGGTCCTCGGGGGTGTCTGTGGGTGGAGGAGCCGGGGTCTGAGCGTCGGAGC CAGCCGCGGGCCAAGAGGGAGCGGAGCCGCCAGCCTCCTCCCGCCGCCCGCAGAGCTGTTTGGATTTGAG GAGGAGCTGCAGAACTGCCTCACGGCAACAGGGATGGGATCAGAGAACAGCGCTTTAAAGAGCTACACAC TGAGGGAGTCTCCTTTTACCTTACCATCTGGACTTGCTGTTTATCCCGCCATATTGCAAGATGGGAAATG TGCTTCCGTGTTTGTATATAAGCGAGAAAATGAAGACAAGGTTAACAAGGCTGCCAAGCACTTAAAGACA CTCCGTCACCCTTGCTTGCTAAGATTTTTATCTTGTACTGTGGAAGCAGATGGCATTCATCTCGTCACTG AGAGAGTGCAGCCTCTGGAAGTGGCCTTGGAAACCCTGTCTCCTGCAGAAGTCTGTGCTGGAATCTATGA CATACTGCTGGCTCTTATCTTCCTTCATGACAGAGGACATTTGACACACAACAATGTCTGCCTGTCGTCT GTTTTTGTGAGTGAGGATGGGCACTGGAAGCTTGGAGGCATGGAGACTGTTTGCCAAGTTCCTCAGGCCA CGCCAGAGTTTCTCAGGAATATCCAGTCAGTAAGAGACCCAGCATCCATTCCTCCTGAGGAGATGTCTCC AGAATTTTCAGGCCTTCCCGAGTCCCACGGCCACGCCCGGGATGCCTATGCCTTTGGAGCTTTGGTGGAC AGCTTGCTCCCCATCTTCAATGAGCAGGTTTCAGCGGACGTTCTCTCCAGCTTCCTACAGATCCTGCACT CAGCTCTGCTGAACCCCATGCCCGAGTGTCGGCCAGCACTCAGCACCTTACTGTCCCATGATTTCTTCAG AAATGATTTTCTAGAAGTTGTGAACTTCTTGAAAAGCTTGACATTAAAGAGTGAAGATGAAAAGACTGAA TTTTTCAAATTTCTGCTGGACCGAGTGAGCTGCCTGTCAGAAGAATTAATAGCTTCAAGATTGGTGCCTC TTCTGCTTAACCAGCTGGTGTTTGCTGAGCCAGTAGCTGTTAAGAGCTTTCTTCCCTATCTACTTGGTCC TAAAAAAGAGAACGCACCAGGAGAAACCCCTTGCTTGCTCTCGCCAGCGCTGTTCCAGTCCCGAGTGATC CCTGTGCTTCTCCGGCTGTTCGAGGTGCACGAGGAGCACGTGCGGATGGTGTTACTGTCCCACATCGAGG CCTACGTGGAGCACTTCACTCAGGAACAGCTAAAGAAAGTCATCCTGCCACAGGTACTACTGGGCCTTCG TGATACCAGCAATTCCATTGTGGCAATTACTCTCCGTAGCCTGGCAGTGCTGGTGTCTTTACTTGGACCA GAAGTGGTTGTGGGAGGAGAACGAACCAAGATCTTTAAGCGCACCGCTCCAAGCTTCACCAAAACTAGTG ACCTTTCCCCTGAAGGTTCTCCCATGCATGTTGTCTGCAGCCAGCAGAGTCGGGTCTCAAAAGTCTTGGA GGACCCCTCGTCTAACGTATTCCCTAAATGGTTGTCTGGCAACGTGCCCAGCAGCAGCAGGAAGCGCATA CAGGAAGAATGTTACAGCTCTCTCTCACAGACAGGTGATCAGTTTTCTCACACCATTAAATTCCCTATGA ATGGACTCTCAGATGTGAAAAACACTTCAGAGGACAATGGAAGCTTCCCTGCAGGTTCTAATAAGCCCGA GGAGTGGCCTGACTGGAGCGAGCCTGAGGAGCCTGAGCAGCAGCCTGCCAGTATTCACAGATGGCCCCGA GAACCCTGTGATGTAGCAGAGTCCCAGCACACAAACCTGACCGCAGAGGAGGTGACCTGGGATGATGGTG AGGCTAGCTTTGGCACTGAAATAAACTCAACAGCTACAGCCTCTGCTCCTGTGCCTGTTACCTCAGGGGG ACAGTCAACCTCTGCAGCTTTGGTTCCTCTCACTGAAGAGTCAAAGCCTTTGCAATCCAGTCCTTCCTCA AAGACCAGTCATAGACAGCATGAGGAAGTCAAACCACCCCAAGTGTCACAAGAAAGACCCCTGAAGGCTC CGTCAGGACTTGGTTTGGGAGAAGAGTTTACCATTCAAGTAAAAAAGAAGCCAGTACAAGACCCGGAGTT GGACTGGTTTGCAGATATGATCCCAGAAATTAAGCCTTCAGGTACTTTTCTTATTCTGCCTGAACTAAGG ACAGAAGTGATGGTCCCTGACAAGGATAACGTCTCATCACTGATGCAGTTTTCCTCAAAGTTTGCTGCAA CAGAAATGACCGAGGGAGAGGCAGAAGGCTGGGAAGGAGAAGAGCTAGCCTGGGAAGATAACAACTGGTG ACGATAATGAAAAGCTTCAGGAAGAATTCTCAAAGAATTAATACCTCAAAGCAAGCTGCATGGATGCAAA ACCCCAAAGCAGCTTGTGTTCCCCACACCAGGAGCTCTTTTTTGCAGTCTGTGCCAAGTCAAGCAGAGAC CAACCTCAGGTTGCTGACTAAGTCCTGGTGGCAAAAGGCTTCGGTGCCAGAGTCCAGGAGAAAGTGCTGT GTGCACTTGACTGAGGCCAGTTTCCATACAAAGCAAACAGCTAAAGAAGTGCTTTTTTCCAGGACAATTC TGGAAGAAATAAAAAATTCAAGCTGGTAAGCTTAATTTTGTGCCATTTCTTTAAAAGAGTAAATTCTATT ATGAAATAACTTAGTGGAAAAAGAAAACAAACAAACCTAGCTGTAAATTATAACTTCAGACTGCTAAGGC TCCTTAGGCAGTTTGTCTGTTTACAGTCTGTCCCTCTCCTTCGGACCTGGGCAGTTACTGTTTCACCTGT ATTACACTGGACCAAAGCTCTCAATGAGAAATACCCACCCTCATCTCCCATCTTGTTCTTTATGTCAAAT AAAGATTATTTTCACATTTACAAAAGCTCTTTTATGTGCTATGAAAATATACAAACATAATTTACATTCA CAATTTCATTTACAAAACACCCAGGCAGGCATCTGAAGAGTCAGCCCAGTTCTGTCGATACACAGGCCAT TCATATTTTACCCTGTAAGCACACAGCGTTTTAGCTTCTCGATCCTTCCTTGCAGCGCCTCCAGTTCTGG CAGCAGCCATGCTGGAGCAAGAGAGTGTTTGAGTAAAAACTCAGTTGTCTCCAAAGCCCTTGCTGCAGCT TTAAATACTGATCTGTATTCTTCTTCAAAGGGCAACACCCGACGGGCTCTCTTTGTGTGAGGCTGGAAAA TAAGGAAATCATGAAACAAGTAATACATAATACTCATTCTAAAAATAATATAAGAGAAACTAGTCATAGG ATCACGAAGAAGGCAGCCTTCCCATTTGTATTAACAATAACCTAAATGTGCTACTACTCTCCACGTCCTA ACTTGTATGTGGCAGACAAGAAGTCGCACTGTGCAACGGTTCCCAATGATAAATGCTGTCTTGTCCAACA ACTATCCACGAGTAGTCTTTAAAAACATCATTAAGGAAATAAAACATGAATTTCCTAATAAGTTTGTAAA TGCTGAGAGCCATAATACCTTTCAACATACTTTTGGAGCCAATTAAGGAAAACTAAGGCTCCAGACATCT TGCATAGAAGGGGAAGAGGAACTGAGGTGAAACTAAAACATATCTTAACAACTAGTTTTTCAAAGGGAAG TCACAGTTTAAATTCATAGGGCTTTCGAACACAACTGACTTAAATTGAACTTTATATATATTCAGGCCTA CAGCTACATCCAAGAAAACAAGTTACTATTGCACTATAAACCTAGAACATTGTTTTCCTTAGGACCCTAT TCTATCACCTGTGTTCAAAACACAATTTTGAATGTATGTAGTCGTGTCAAATTTCTGCCAATTAATAGGT ACATGTGATATTTGAGAAAAGTGATTATTATTGATGAGATGAATTGAACAAACTGACATCTAAGTGTCAG AAAATGCATTTATGTCATCATTGGTACAATATGCAATTACCAATAATAAAGAGTATATTTGGTACAATGG TTACAATTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6417440 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6417440 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7679 same embryo
	EMAGE:7681 same embryo
	EMAGE:7680 same embryo
	EurExpress:euxassay_005290 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source