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EMAGE:7683

Ppp1r3f protein phosphatase 1, regulatory (inhibitor) subunit 3F ( MGI:1859617)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7683 EMAGE:7683 EMAGE:7683 EMAGE:7683 EMAGE:7683
"Pseudo-wholemount" of euxassay_009515. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009515_01 euxassay_009515_02 euxassay_009515_03 euxassay_009515_04
EMAGE:7683 EMAGE:7683 EMAGE:7683 EMAGE:7683 EMAGE:7683
euxassay_009515_05 euxassay_009515_06 euxassay_009515_07 euxassay_009515_08 euxassay_009515_09
EMAGE:7683 EMAGE:7683 EMAGE:7683 EMAGE:7683 EMAGE:7683
euxassay_009515_10 euxassay_009515_11 euxassay_009515_12 euxassay_009515_13 euxassay_009515_14
EMAGE:7683 EMAGE:7683 EMAGE:7683 EMAGE:7683 EMAGE:7683
euxassay_009515_15 euxassay_009515_16 euxassay_009515_17 euxassay_009515_18 euxassay_009515_19
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euxassay_009515_20 euxassay_009515_21 euxassay_009515_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7683Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7683_wholemount_strong.wlz
7683_wholemount_moderate.wlz
7683_wholemount_weak.wlz
7683_wholemount_possible.wlz
7683_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7683_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
left lung
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 09 10 weak expression: see section 06 07 08
right lung
moderate moderate
regionalmoderate expression: see section 13 14 15 16 19 20 weak expression: see section 11 12 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36888
Entity Detected:Ppp1r3f, protein phosphatase 1, regulatory (inhibitor) subunit 3F ( MGI:1859617)
Sequence:sense strand is shown

>T36888
AAGACACCATTGACCAGGAGTTGGAGCAGCTCTACCTGTCTCACCTGAGCCGCTTGAGAGCTGTGGCAGC
GGCGGGTGGCGGGGAAGGCACTTCCCCCACTCATGCCCTGGGCATACTCACAGACCGTGACCTGATCTTG
AAGTGGCCTGGCCCCGAGAGGGCCCTGAACAGTGCTCTGGCCGAGGAGATCACGCTGCACTATGCGCGCC
TGGGCTGTGGCGTGGAGCTCATCAAGGACACTGAAGACCCAGATGACGAGGGGGAGGGTGAAGATGGGCT
CTCCATCACTCCTTCTAGCCCTGAAGGGGGCAGCCCTAAGGAATCGCCTCCAGAAATCCTCTCTGGAGCC
CGCTCTGTGATAGCTACTATGGGAGATGTGTGGGTTCCATGGGCAGAGCGCTCTAGCTCTAGATGTGATA
GCCCAGTGGTTCTGGGAACACAGGGTCAGTTCACTGAGAATCCAGAGAAAGGGATGGGCAAGGACACCAA
GTCTCTGCACCTGAATAGGGTAATAGTTGGGATGAGCAAGTCCCCTGGGGAGGCTGGGACGGAAAGCCAG
ATGGAGGAGCTACCTACTGAGCGGGAAAGCAGCTGGGTACCTAGTTCTGAGAAAGAGCTGCCTCTACCAG
TCCAGCAAGAGCAGAGTCCTGCCCTCCTTGGTCCCACAGGGACTGAAGTCTGTCTGTCTAGTGTAGCCAA
GCCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66785. Forward Primer - name:066785_F_cDNA_Ppp1r3f, sequence:AAGACACCATTGACCAGGAGTT; Reverse Primer - name:066785_N_SP6_cDNA_Ppp1r3f, sequence:AGGCTTGGCTACACTAGACAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7687 same embryo
 EMAGE:7684 same embryo
 EMAGE:7685 same embryo
 EMAGE:7686 same embryo
 EMAGE:7682 same embryo
 EurExpress:euxassay_009515 same experiment
 MGI:4827378 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS